Genome-Wide Investigation of Genes Regulated by ERα in Breast Cancer Cells
Estrogen receptor alpha (ERα), which has been detected in over 70% of breast cancer cases, is a driving factor for breast cancer growth. For investigating the underlying genes and networks regulated by ERα in breast cancer, RNA-seq was performed between ERα transgenic M...
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| Format: | Article |
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MDPI AG
2018-10-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/23/10/2543 |
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| author | Shuning Wang Xiaoju Li Wangqian Zhang Yuan Gao Kuo Zhang Qiang Hao Weina Li Zhaowei Wang Meng Li Wei Zhang Yingqi Zhang Cun Zhang |
| author_facet | Shuning Wang Xiaoju Li Wangqian Zhang Yuan Gao Kuo Zhang Qiang Hao Weina Li Zhaowei Wang Meng Li Wei Zhang Yingqi Zhang Cun Zhang |
| author_sort | Shuning Wang |
| collection | DOAJ |
| description | Estrogen receptor alpha (ERα), which has been detected in over 70% of breast cancer cases, is a driving factor for breast cancer growth. For investigating the underlying genes and networks regulated by ERα in breast cancer, RNA-seq was performed between ERα transgenic MDA-MB-231 cells and wild type MDA-MB-231 cells. A total of 267 differentially expressed genes (DEGs) were identified. Then bioinformatics analyses were performed to illustrate the mechanism of ERα. Besides, by comparison of RNA-seq data obtained from MDA-MB-231 cells and microarray dataset obtained from estrogen (E2) stimulated MCF-7 cells, an overlap of 126 DEGs was screened. The expression level of ERα was negatively associated with metastasis and EMT in breast cancer. We further verified that ERα might inhibit metastasis by regulating of VCL and TNFRSF12A, and suppress EMT by the regulating of JUNB and ID3. And the relationship between ERα and these genes were validated by RT-PCR and correlation analysis based on TCGA database. By PPI network analysis, we identified TOP5 hub genes, FOS, SP1, CDKN1A, CALCR and JUNB, which were involved in cell proliferation and invasion. Taken together, the whole-genome insights carried in this work can help fully understanding biological roles of ERα in breast cancer. |
| first_indexed | 2024-12-10T21:01:56Z |
| format | Article |
| id | doaj.art-a0b9662cbce248f68211a2b9af53900f |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T21:01:56Z |
| publishDate | 2018-10-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a0b9662cbce248f68211a2b9af53900f2022-12-22T01:33:47ZengMDPI AGMolecules1420-30492018-10-012310254310.3390/molecules23102543molecules23102543Genome-Wide Investigation of Genes Regulated by ERα in Breast Cancer CellsShuning Wang0Xiaoju Li1Wangqian Zhang2Yuan Gao3Kuo Zhang4Qiang Hao5Weina Li6Zhaowei Wang7Meng Li8Wei Zhang9Yingqi Zhang10Cun Zhang11State Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, The Fourth Military Medical University, Xi’an 710032, ChinaState Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, The Fourth Military Medical University, Xi’an 710032, ChinaState Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, The Fourth Military Medical University, Xi’an 710032, ChinaState Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, The Fourth Military Medical University, Xi’an 710032, ChinaState Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, The Fourth Military Medical University, Xi’an 710032, ChinaState Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, The Fourth Military Medical University, Xi’an 710032, ChinaState Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, The Fourth Military Medical University, Xi’an 710032, ChinaState Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, The Fourth Military Medical University, Xi’an 710032, ChinaState Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, The Fourth Military Medical University, Xi’an 710032, ChinaState Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, The Fourth Military Medical University, Xi’an 710032, ChinaState Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, The Fourth Military Medical University, Xi’an 710032, ChinaState Key Laboratory of Cancer Biology, Biotechnology Center, School of Pharmacy, The Fourth Military Medical University, Xi’an 710032, ChinaEstrogen receptor alpha (ERα), which has been detected in over 70% of breast cancer cases, is a driving factor for breast cancer growth. For investigating the underlying genes and networks regulated by ERα in breast cancer, RNA-seq was performed between ERα transgenic MDA-MB-231 cells and wild type MDA-MB-231 cells. A total of 267 differentially expressed genes (DEGs) were identified. Then bioinformatics analyses were performed to illustrate the mechanism of ERα. Besides, by comparison of RNA-seq data obtained from MDA-MB-231 cells and microarray dataset obtained from estrogen (E2) stimulated MCF-7 cells, an overlap of 126 DEGs was screened. The expression level of ERα was negatively associated with metastasis and EMT in breast cancer. We further verified that ERα might inhibit metastasis by regulating of VCL and TNFRSF12A, and suppress EMT by the regulating of JUNB and ID3. And the relationship between ERα and these genes were validated by RT-PCR and correlation analysis based on TCGA database. By PPI network analysis, we identified TOP5 hub genes, FOS, SP1, CDKN1A, CALCR and JUNB, which were involved in cell proliferation and invasion. Taken together, the whole-genome insights carried in this work can help fully understanding biological roles of ERα in breast cancer.http://www.mdpi.com/1420-3049/23/10/2543genesestrogen receptor alpha (ERα)breast cancerRNA-seqdifferentially expressed genes (DEGs)PPI networks |
| spellingShingle | Shuning Wang Xiaoju Li Wangqian Zhang Yuan Gao Kuo Zhang Qiang Hao Weina Li Zhaowei Wang Meng Li Wei Zhang Yingqi Zhang Cun Zhang Genome-Wide Investigation of Genes Regulated by ERα in Breast Cancer Cells Molecules genes estrogen receptor alpha (ERα) breast cancer RNA-seq differentially expressed genes (DEGs) PPI networks |
| title | Genome-Wide Investigation of Genes Regulated by ERα in Breast Cancer Cells |
| title_full | Genome-Wide Investigation of Genes Regulated by ERα in Breast Cancer Cells |
| title_fullStr | Genome-Wide Investigation of Genes Regulated by ERα in Breast Cancer Cells |
| title_full_unstemmed | Genome-Wide Investigation of Genes Regulated by ERα in Breast Cancer Cells |
| title_short | Genome-Wide Investigation of Genes Regulated by ERα in Breast Cancer Cells |
| title_sort | genome wide investigation of genes regulated by erα in breast cancer cells |
| topic | genes estrogen receptor alpha (ERα) breast cancer RNA-seq differentially expressed genes (DEGs) PPI networks |
| url | http://www.mdpi.com/1420-3049/23/10/2543 |
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