Serological Diagnosis of Acute Scrub Typhus in Southern India: Evaluation of InBios Scrub Typhus Detect IgM Rapid Test and Comparison with other Serological Tests
Introduction: Scrub Typhus (ST) is being reported from different parts of India in the recent past. However, the diagnosis and confirmation of ST cases require specific serological and molecular diagnostic tests. Both rapid and conventional ELISA tests need to be properly evaluated. Aim: Evalua...
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| Format: | Article |
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JCDR Research and Publications Private Limited
2016-11-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/8861/24051_CE(Ra1)_F(Sh)_PF1(PI_VJ)_PFA(AK)_PF2(PAG).pdf |
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| author | Velmurugan Anitharaj Selvaraj Stephen Jothimani Pradeep Sungman Park Seung-Han Kim Young Jin Kim Eun-Ye Kim Yoon-Won Kim |
| author_facet | Velmurugan Anitharaj Selvaraj Stephen Jothimani Pradeep Sungman Park Seung-Han Kim Young Jin Kim Eun-Ye Kim Yoon-Won Kim |
| author_sort | Velmurugan Anitharaj |
| collection | DOAJ |
| description | Introduction: Scrub Typhus (ST) is being reported from different
parts of India in the recent past. However, the diagnosis and
confirmation of ST cases require specific serological and
molecular diagnostic tests. Both rapid and conventional ELISA
tests need to be properly evaluated.
Aim: Evaluation of a new ST IgM Immunochromatography
(ICT) test kit (InBios Scrub Typhus Detect IgM Rapid Test) and
compare it with another rapid kit, conventional ELISA kit and
Weil-Felix (WF) test.
Materials and Methods: This prospective study was carried
out in Mahatma Gandhi Medical College and Research Institute,
Puducherry, during November 2015 to June 2016. Clinically
suspected 220 ST patients were examined by a new kit, InBios
Scrub Typhus Detect IgM Rapid Test, taking the conventional
InBios Scrub Typhus Detect IgM ELISA as reference. Additional
comparison was made with ImmuneMed Scrub Typhus Rapid,
and WF test (single OXK titers ≥1:320). Statistical analysis was
performed (Chi-square, Spearman’s correlation and Kappa)
using IBM SPSS Statistics 17 for Windows (SPSS Inc; Chicago,
USA).
Results: Percentage Sensitivity, Specificity, Positive Predictive
and Negative Predictive Values for InBios, ImmuneMed and WF
were 99.25, 93.02, 95.68, 98.77; 94.87, 94.19, 96.21, 92.05 and
50.38, 95.51, 94.29, 56.67 respectively. A total of 134 patients
were positive in reference standard InBios IgM ELISA.
Conclusion: This new rapid ST IgM kit validated for the first
time in India, showed good sensitivity and specificity. As a
Point-of-Care (PoC) test, the kit would be helpful in both urban
and remote rural parts of India. |
| first_indexed | 2024-12-14T11:40:44Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-14T11:40:44Z |
| publishDate | 2016-11-01 |
| publisher | JCDR Research and Publications Private Limited |
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| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-a0b979b2aa2a48129cda19ed37978c2f2022-12-21T23:02:50ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2016-11-011011DC07DC1010.7860/JCDR/2016/24051.8861Serological Diagnosis of Acute Scrub Typhus in Southern India: Evaluation of InBios Scrub Typhus Detect IgM Rapid Test and Comparison with other Serological TestsVelmurugan Anitharaj0Selvaraj Stephen1Jothimani Pradeep2Sungman Park3Seung-Han Kim4Young Jin Kim5Eun-Ye Kim6Yoon-Won Kim7Ph.D. Scholar, Department of Microbiology, Mahatma Gandhi Medical College and Research Institute, Puducherry, India.Professor, Department of Microbiology, Mahatma Gandhi Medical College and Research Institute, Puducherry, India.Ph.D. Scholar, Department of Microbiology, Mahatma Gandhi Medical College and Research Institute, Puducherry, India.Scientist, ImmuneMed Inc., Chuncheon, Gangwon Do, South Korea.Scientist, ImmuneMed Inc., Chuncheon, Gangwon Do, South Korea.Scientist, ImmuneMed Inc., Chuncheon, Gangwon Do, South Korea.Scientist, ImmuneMed Inc., Chuncheon, Gangwon Do, South Korea.Teaching Faculty, Department of Microbiology, Hallym University College of Medicine, Chuncheon, Gangwon do, South Korea.Introduction: Scrub Typhus (ST) is being reported from different parts of India in the recent past. However, the diagnosis and confirmation of ST cases require specific serological and molecular diagnostic tests. Both rapid and conventional ELISA tests need to be properly evaluated. Aim: Evaluation of a new ST IgM Immunochromatography (ICT) test kit (InBios Scrub Typhus Detect IgM Rapid Test) and compare it with another rapid kit, conventional ELISA kit and Weil-Felix (WF) test. Materials and Methods: This prospective study was carried out in Mahatma Gandhi Medical College and Research Institute, Puducherry, during November 2015 to June 2016. Clinically suspected 220 ST patients were examined by a new kit, InBios Scrub Typhus Detect IgM Rapid Test, taking the conventional InBios Scrub Typhus Detect IgM ELISA as reference. Additional comparison was made with ImmuneMed Scrub Typhus Rapid, and WF test (single OXK titers ≥1:320). Statistical analysis was performed (Chi-square, Spearman’s correlation and Kappa) using IBM SPSS Statistics 17 for Windows (SPSS Inc; Chicago, USA). Results: Percentage Sensitivity, Specificity, Positive Predictive and Negative Predictive Values for InBios, ImmuneMed and WF were 99.25, 93.02, 95.68, 98.77; 94.87, 94.19, 96.21, 92.05 and 50.38, 95.51, 94.29, 56.67 respectively. A total of 134 patients were positive in reference standard InBios IgM ELISA. Conclusion: This new rapid ST IgM kit validated for the first time in India, showed good sensitivity and specificity. As a Point-of-Care (PoC) test, the kit would be helpful in both urban and remote rural parts of India.https://jcdr.net/articles/PDF/8861/24051_CE(Ra1)_F(Sh)_PF1(PI_VJ)_PFA(AK)_PF2(PAG).pdfenzyme-linked immunosorbent assayimmunochromatographyorientia tsutsugamushi |
| spellingShingle | Velmurugan Anitharaj Selvaraj Stephen Jothimani Pradeep Sungman Park Seung-Han Kim Young Jin Kim Eun-Ye Kim Yoon-Won Kim Serological Diagnosis of Acute Scrub Typhus in Southern India: Evaluation of InBios Scrub Typhus Detect IgM Rapid Test and Comparison with other Serological Tests Journal of Clinical and Diagnostic Research enzyme-linked immunosorbent assay immunochromatography orientia tsutsugamushi |
| title | Serological Diagnosis of Acute Scrub Typhus in Southern India: Evaluation of InBios Scrub Typhus Detect IgM Rapid Test and Comparison with other Serological Tests |
| title_full | Serological Diagnosis of Acute Scrub Typhus in Southern India: Evaluation of InBios Scrub Typhus Detect IgM Rapid Test and Comparison with other Serological Tests |
| title_fullStr | Serological Diagnosis of Acute Scrub Typhus in Southern India: Evaluation of InBios Scrub Typhus Detect IgM Rapid Test and Comparison with other Serological Tests |
| title_full_unstemmed | Serological Diagnosis of Acute Scrub Typhus in Southern India: Evaluation of InBios Scrub Typhus Detect IgM Rapid Test and Comparison with other Serological Tests |
| title_short | Serological Diagnosis of Acute Scrub Typhus in Southern India: Evaluation of InBios Scrub Typhus Detect IgM Rapid Test and Comparison with other Serological Tests |
| title_sort | serological diagnosis of acute scrub typhus in southern india evaluation of inbios scrub typhus detect igm rapid test and comparison with other serological tests |
| topic | enzyme-linked immunosorbent assay immunochromatography orientia tsutsugamushi |
| url | https://jcdr.net/articles/PDF/8861/24051_CE(Ra1)_F(Sh)_PF1(PI_VJ)_PFA(AK)_PF2(PAG).pdf |
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