PRAME Immunocytochemistry for the Diagnosis of Melanoma Metastases in Cytological Samples
(1) Background: Fine-needle aspiration cytology is often used for the pre-operative diagnosis of melanoma metastases. The diagnosis may not be confidently established based on morphology alone, and immunocytochemistry is mandatory. The choice of the most advantageous immunocytochemical antibodies is...
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MDPI AG
2022-03-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/12/3/646 |
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| author | Andrea Ronchi Federica Zito Marino Elvira Moscarella Gabriella Brancaccio Giuseppe Argenziano Teresa Troiani Stefania Napolitano Renato Franco Immacolata Cozzolino |
| author_facet | Andrea Ronchi Federica Zito Marino Elvira Moscarella Gabriella Brancaccio Giuseppe Argenziano Teresa Troiani Stefania Napolitano Renato Franco Immacolata Cozzolino |
| author_sort | Andrea Ronchi |
| collection | DOAJ |
| description | (1) Background: Fine-needle aspiration cytology is often used for the pre-operative diagnosis of melanoma metastases. The diagnosis may not be confidently established based on morphology alone, and immunocytochemistry is mandatory. The choice of the most advantageous immunocytochemical antibodies is critical, as the sample may be scant, and the presence of pigmented histiocytes may be confounding. However, the diagnostic performance of melanocytic markers in this setting is poorly investigated. Moreover, PRAME (preferentially expressed antigen in melanoma) recently emerged as a novel marker for the diagnosis of melanoma. The current work aimed to evaluate the sensitivity and specificity of PRAME for the diagnosis of melanoma metastases in cytological samples, compared to other melanocytic markers. (2) Methods: PRAME, S100, Melan-A, HMB45 and SOX10 were tested on cell block sections of 48 cases of melanoma metastases diagnosed from cytological samples, and 20 cases of reactive lymphadenopathy. (3) Results: S100 and SOX10 showed the highest sensitivity (100%), while the sensitivity of PRAME was 85.4%. PRAME, Melan-A, SOX10 and HMB45 showed a specificity of 100%, while the specificity of S100 was lower (85%), as it marked some histiocytes. (4) Conclusion: PRAME immunocytochemistry is highly specific for the diagnosis of melanoma metastasis from a cytological sample, but is less sensitive compared with other melanocytic markers. |
| first_indexed | 2024-03-09T13:46:22Z |
| format | Article |
| id | doaj.art-a0bc5b020fdb4847babac0dd6b2433ce |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-09T13:46:22Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-a0bc5b020fdb4847babac0dd6b2433ce2023-11-30T20:59:00ZengMDPI AGDiagnostics2075-44182022-03-0112364610.3390/diagnostics12030646PRAME Immunocytochemistry for the Diagnosis of Melanoma Metastases in Cytological SamplesAndrea Ronchi0Federica Zito Marino1Elvira Moscarella2Gabriella Brancaccio3Giuseppe Argenziano4Teresa Troiani5Stefania Napolitano6Renato Franco7Immacolata Cozzolino8Pathology Unit, Department of Mental and Physical Health and Preventive Medicine, Università degli Studi della Campania “Luigi Vanvitelli”, Via Luciano Armanni 5, 80100 Naples, ItalyPathology Unit, Department of Mental and Physical Health and Preventive Medicine, Università degli Studi della Campania “Luigi Vanvitelli”, Via Luciano Armanni 5, 80100 Naples, ItalyDermatology Unit, Department of Mental and Physical Health and Preventive Medicine, Università degli Studi della Campania “Luigi Vanvitelli”, Via Luciano Armanni 5, 80100 Naples, ItalyDermatology Unit, Department of Mental and Physical Health and Preventive Medicine, Università degli Studi della Campania “Luigi Vanvitelli”, Via Luciano Armanni 5, 80100 Naples, ItalyDermatology Unit, Department of Mental and Physical Health and Preventive Medicine, Università degli Studi della Campania “Luigi Vanvitelli”, Via Luciano Armanni 5, 80100 Naples, ItalyOncology Unit, Department of Precision Medicine, Università degli Studi della Campania “Luigi Vanvitelli”, Via Luciano Armanni 5, 80100 Naples, ItalyOncology Unit, Department of Precision Medicine, Università degli Studi della Campania “Luigi Vanvitelli”, Via Luciano Armanni 5, 80100 Naples, ItalyPathology Unit, Department of Mental and Physical Health and Preventive Medicine, Università degli Studi della Campania “Luigi Vanvitelli”, Via Luciano Armanni 5, 80100 Naples, ItalyPathology Unit, Department of Mental and Physical Health and Preventive Medicine, Università degli Studi della Campania “Luigi Vanvitelli”, Via Luciano Armanni 5, 80100 Naples, Italy(1) Background: Fine-needle aspiration cytology is often used for the pre-operative diagnosis of melanoma metastases. The diagnosis may not be confidently established based on morphology alone, and immunocytochemistry is mandatory. The choice of the most advantageous immunocytochemical antibodies is critical, as the sample may be scant, and the presence of pigmented histiocytes may be confounding. However, the diagnostic performance of melanocytic markers in this setting is poorly investigated. Moreover, PRAME (preferentially expressed antigen in melanoma) recently emerged as a novel marker for the diagnosis of melanoma. The current work aimed to evaluate the sensitivity and specificity of PRAME for the diagnosis of melanoma metastases in cytological samples, compared to other melanocytic markers. (2) Methods: PRAME, S100, Melan-A, HMB45 and SOX10 were tested on cell block sections of 48 cases of melanoma metastases diagnosed from cytological samples, and 20 cases of reactive lymphadenopathy. (3) Results: S100 and SOX10 showed the highest sensitivity (100%), while the sensitivity of PRAME was 85.4%. PRAME, Melan-A, SOX10 and HMB45 showed a specificity of 100%, while the specificity of S100 was lower (85%), as it marked some histiocytes. (4) Conclusion: PRAME immunocytochemistry is highly specific for the diagnosis of melanoma metastasis from a cytological sample, but is less sensitive compared with other melanocytic markers.https://www.mdpi.com/2075-4418/12/3/646melanoma metastasisimmunocytochemistrySOX10S100Melan-AHMB45 |
| spellingShingle | Andrea Ronchi Federica Zito Marino Elvira Moscarella Gabriella Brancaccio Giuseppe Argenziano Teresa Troiani Stefania Napolitano Renato Franco Immacolata Cozzolino PRAME Immunocytochemistry for the Diagnosis of Melanoma Metastases in Cytological Samples Diagnostics melanoma metastasis immunocytochemistry SOX10 S100 Melan-A HMB45 |
| title | PRAME Immunocytochemistry for the Diagnosis of Melanoma Metastases in Cytological Samples |
| title_full | PRAME Immunocytochemistry for the Diagnosis of Melanoma Metastases in Cytological Samples |
| title_fullStr | PRAME Immunocytochemistry for the Diagnosis of Melanoma Metastases in Cytological Samples |
| title_full_unstemmed | PRAME Immunocytochemistry for the Diagnosis of Melanoma Metastases in Cytological Samples |
| title_short | PRAME Immunocytochemistry for the Diagnosis of Melanoma Metastases in Cytological Samples |
| title_sort | prame immunocytochemistry for the diagnosis of melanoma metastases in cytological samples |
| topic | melanoma metastasis immunocytochemistry SOX10 S100 Melan-A HMB45 |
| url | https://www.mdpi.com/2075-4418/12/3/646 |
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