Silencing of circ_0000517 suppresses proliferation, glycolysis, and glutamine decomposition of non‐small cell lung cancer by modulating miR‐330‐5p/YY1 signal pathway
Abstract In recent years, circular RNA (circRNA) has been found to be involved in a variety of cancer processes. More and more attention has been paid to the research of circRNA in lung cancer. This study aims to investigate whether circ_0000517 affected the physiology of non‐small cell lung cancer...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-12-01
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| Series: | Kaohsiung Journal of Medical Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/kjm2.12440 |
| _version_ | 1818834244395335680 |
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| author | Zhong‐Xing Bing Jia‐Qi Zhang Gui‐Ge Wang Yan‐Qing Wang Tian‐Ge Wang Dan‐Qing Li |
| author_facet | Zhong‐Xing Bing Jia‐Qi Zhang Gui‐Ge Wang Yan‐Qing Wang Tian‐Ge Wang Dan‐Qing Li |
| author_sort | Zhong‐Xing Bing |
| collection | DOAJ |
| description | Abstract In recent years, circular RNA (circRNA) has been found to be involved in a variety of cancer processes. More and more attention has been paid to the research of circRNA in lung cancer. This study aims to investigate whether circ_0000517 affected the physiology of non‐small cell lung cancer (NSCLC) and the underlying mechanism. The results demonstrated that circ_0000517 was highly expressed in lung cancer tissues and cells, and overexpression of circ_0000517 was negatively correlated with the prognosis of NSCLC patients. Silencing of circ_0000517 significantly inhibited the proliferation, glycolysis, and glutamine decomposition of NSCLC cells in vitro and repressed the growth of xenografted tumors in vivo. Moreover, knockdown of circ_0000517 attenuated the expression of PCNA, HK2, LDHA, ASCT2, and GLS1. Further study found that circ_0000517 targeted miR‐330‐5p and miR‐330‐5p targeted YY1. In addition, miR‐330‐5p inhibitor reversed inhibition of cancer cell proliferation, glycolysis, and glutamine decomposition induced by si‐circ_0000517. In conclusion, our study revealed that silencing of circ_0000517 improved the progression of NSCLC through regulating miR‐330‐5p/YY1 axis. |
| first_indexed | 2024-12-19T02:31:44Z |
| format | Article |
| id | doaj.art-a0bc8c2ce55241e0a7679816761c679a |
| institution | Directory Open Access Journal |
| issn | 1607-551X 2410-8650 |
| language | English |
| last_indexed | 2024-12-19T02:31:44Z |
| publishDate | 2021-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-a0bc8c2ce55241e0a7679816761c679a2022-12-21T20:39:37ZengWileyKaohsiung Journal of Medical Sciences1607-551X2410-86502021-12-0137121027103710.1002/kjm2.12440Silencing of circ_0000517 suppresses proliferation, glycolysis, and glutamine decomposition of non‐small cell lung cancer by modulating miR‐330‐5p/YY1 signal pathwayZhong‐Xing Bing0Jia‐Qi Zhang1Gui‐Ge Wang2Yan‐Qing Wang3Tian‐Ge Wang4Dan‐Qing Li5Department of Thoracic Surgery Peking Union Medical College Hospital Beijing ChinaDepartment of Thoracic Surgery Peking Union Medical College Hospital Beijing ChinaDepartment of Thoracic Surgery Peking Union Medical College Hospital Beijing ChinaDepartment of Thoracic Surgery Peking Union Medical College Hospital Beijing ChinaChinese Academy of Medical Sciences Plastic Surgery Hospital Beijing ChinaDepartment of Thoracic Surgery Peking Union Medical College Hospital Beijing ChinaAbstract In recent years, circular RNA (circRNA) has been found to be involved in a variety of cancer processes. More and more attention has been paid to the research of circRNA in lung cancer. This study aims to investigate whether circ_0000517 affected the physiology of non‐small cell lung cancer (NSCLC) and the underlying mechanism. The results demonstrated that circ_0000517 was highly expressed in lung cancer tissues and cells, and overexpression of circ_0000517 was negatively correlated with the prognosis of NSCLC patients. Silencing of circ_0000517 significantly inhibited the proliferation, glycolysis, and glutamine decomposition of NSCLC cells in vitro and repressed the growth of xenografted tumors in vivo. Moreover, knockdown of circ_0000517 attenuated the expression of PCNA, HK2, LDHA, ASCT2, and GLS1. Further study found that circ_0000517 targeted miR‐330‐5p and miR‐330‐5p targeted YY1. In addition, miR‐330‐5p inhibitor reversed inhibition of cancer cell proliferation, glycolysis, and glutamine decomposition induced by si‐circ_0000517. In conclusion, our study revealed that silencing of circ_0000517 improved the progression of NSCLC through regulating miR‐330‐5p/YY1 axis.https://doi.org/10.1002/kjm2.12440circ_0000517glycolysismiR‐330‐5pNSCLCYY1 |
| spellingShingle | Zhong‐Xing Bing Jia‐Qi Zhang Gui‐Ge Wang Yan‐Qing Wang Tian‐Ge Wang Dan‐Qing Li Silencing of circ_0000517 suppresses proliferation, glycolysis, and glutamine decomposition of non‐small cell lung cancer by modulating miR‐330‐5p/YY1 signal pathway Kaohsiung Journal of Medical Sciences circ_0000517 glycolysis miR‐330‐5p NSCLC YY1 |
| title | Silencing of circ_0000517 suppresses proliferation, glycolysis, and glutamine decomposition of non‐small cell lung cancer by modulating miR‐330‐5p/YY1 signal pathway |
| title_full | Silencing of circ_0000517 suppresses proliferation, glycolysis, and glutamine decomposition of non‐small cell lung cancer by modulating miR‐330‐5p/YY1 signal pathway |
| title_fullStr | Silencing of circ_0000517 suppresses proliferation, glycolysis, and glutamine decomposition of non‐small cell lung cancer by modulating miR‐330‐5p/YY1 signal pathway |
| title_full_unstemmed | Silencing of circ_0000517 suppresses proliferation, glycolysis, and glutamine decomposition of non‐small cell lung cancer by modulating miR‐330‐5p/YY1 signal pathway |
| title_short | Silencing of circ_0000517 suppresses proliferation, glycolysis, and glutamine decomposition of non‐small cell lung cancer by modulating miR‐330‐5p/YY1 signal pathway |
| title_sort | silencing of circ 0000517 suppresses proliferation glycolysis and glutamine decomposition of non small cell lung cancer by modulating mir 330 5p yy1 signal pathway |
| topic | circ_0000517 glycolysis miR‐330‐5p NSCLC YY1 |
| url | https://doi.org/10.1002/kjm2.12440 |
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