Characterization of a New and Efficient Polyvalent Phage Infecting <i>E. coli</i> O157:H7, <i>Salmonella</i> spp., and <i>Shigella sonnei</i>
Ongoing outbreaks of foodborne diseases remain a significant public health concern. Lytic phages provide promising attributes as biocontrol agents. This study characterized KFS-EC3, a polyvalent and lytic phage, which was isolated from slaughterhouse sewage and purified by cesium chloride density ce...
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| Format: | Article |
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MDPI AG
2021-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/10/2105 |
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| author | Su-Hyeon Kim Damilare Emmanuel Adeyemi Mi-Kyung Park |
| author_facet | Su-Hyeon Kim Damilare Emmanuel Adeyemi Mi-Kyung Park |
| author_sort | Su-Hyeon Kim |
| collection | DOAJ |
| description | Ongoing outbreaks of foodborne diseases remain a significant public health concern. Lytic phages provide promising attributes as biocontrol agents. This study characterized KFS-EC3, a polyvalent and lytic phage, which was isolated from slaughterhouse sewage and purified by cesium chloride density centrifugation. Host range and efficiency of plating analyses revealed that KFS-EC3 is polyvalent and can efficiently infect <i>E. coli</i> O157:H7, <i>Salmonella</i> spp., and <i>Shigella sonnei</i>. KFS-EC3 had a latent time of 20 min and burst size of ~71 phages/infected cell. KFS-EC3 was stable and infectious following storage at a pH range of 3 to 11 and a temperature range of −70 °C to 60 °C. KFS-EC3 could inhibit <i>E. coli</i> O157:H7 growth by 2 logs up to 52 h even at the lowest MOI of 0.001. Genomic analysis of KFS-EC3 revealed that it consisted of 167,440 bp and 273 ORFs identified as functional genes, without any genes associated with antibiotic resistance, virulence, allergenicity, and lysogenicity. This phage was finally classified into the <i>Tequa</i><i>trovirus</i> genus of the <i>Myoviridae</i> family. In conclusion, KFS-EC3 could simultaneously infect <i>E. coli</i> O157:H7, <i>S</i>. <i>sonnei</i>, and <i>Salmonella</i> spp. with the lowest MOI values over long periods, suggesting its suitability for simultaneous pathogen control in foods. |
| first_indexed | 2024-03-10T06:22:16Z |
| format | Article |
| id | doaj.art-a0be019098ea4677b7f0fa2831bb5997 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T06:22:16Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-a0be019098ea4677b7f0fa2831bb59972023-11-22T19:14:22ZengMDPI AGMicroorganisms2076-26072021-10-01910210510.3390/microorganisms9102105Characterization of a New and Efficient Polyvalent Phage Infecting <i>E. coli</i> O157:H7, <i>Salmonella</i> spp., and <i>Shigella sonnei</i>Su-Hyeon Kim0Damilare Emmanuel Adeyemi1Mi-Kyung Park2School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, KoreaSchool of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, KoreaSchool of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, KoreaOngoing outbreaks of foodborne diseases remain a significant public health concern. Lytic phages provide promising attributes as biocontrol agents. This study characterized KFS-EC3, a polyvalent and lytic phage, which was isolated from slaughterhouse sewage and purified by cesium chloride density centrifugation. Host range and efficiency of plating analyses revealed that KFS-EC3 is polyvalent and can efficiently infect <i>E. coli</i> O157:H7, <i>Salmonella</i> spp., and <i>Shigella sonnei</i>. KFS-EC3 had a latent time of 20 min and burst size of ~71 phages/infected cell. KFS-EC3 was stable and infectious following storage at a pH range of 3 to 11 and a temperature range of −70 °C to 60 °C. KFS-EC3 could inhibit <i>E. coli</i> O157:H7 growth by 2 logs up to 52 h even at the lowest MOI of 0.001. Genomic analysis of KFS-EC3 revealed that it consisted of 167,440 bp and 273 ORFs identified as functional genes, without any genes associated with antibiotic resistance, virulence, allergenicity, and lysogenicity. This phage was finally classified into the <i>Tequa</i><i>trovirus</i> genus of the <i>Myoviridae</i> family. In conclusion, KFS-EC3 could simultaneously infect <i>E. coli</i> O157:H7, <i>S</i>. <i>sonnei</i>, and <i>Salmonella</i> spp. with the lowest MOI values over long periods, suggesting its suitability for simultaneous pathogen control in foods.https://www.mdpi.com/2076-2607/9/10/2105polyvalent phageeffectiveness<i>Escherichia coli</i> O157:H7<i>Salmonella</i><i>Shigella sonnei</i> |
| spellingShingle | Su-Hyeon Kim Damilare Emmanuel Adeyemi Mi-Kyung Park Characterization of a New and Efficient Polyvalent Phage Infecting <i>E. coli</i> O157:H7, <i>Salmonella</i> spp., and <i>Shigella sonnei</i> Microorganisms polyvalent phage effectiveness <i>Escherichia coli</i> O157:H7 <i>Salmonella</i> <i>Shigella sonnei</i> |
| title | Characterization of a New and Efficient Polyvalent Phage Infecting <i>E. coli</i> O157:H7, <i>Salmonella</i> spp., and <i>Shigella sonnei</i> |
| title_full | Characterization of a New and Efficient Polyvalent Phage Infecting <i>E. coli</i> O157:H7, <i>Salmonella</i> spp., and <i>Shigella sonnei</i> |
| title_fullStr | Characterization of a New and Efficient Polyvalent Phage Infecting <i>E. coli</i> O157:H7, <i>Salmonella</i> spp., and <i>Shigella sonnei</i> |
| title_full_unstemmed | Characterization of a New and Efficient Polyvalent Phage Infecting <i>E. coli</i> O157:H7, <i>Salmonella</i> spp., and <i>Shigella sonnei</i> |
| title_short | Characterization of a New and Efficient Polyvalent Phage Infecting <i>E. coli</i> O157:H7, <i>Salmonella</i> spp., and <i>Shigella sonnei</i> |
| title_sort | characterization of a new and efficient polyvalent phage infecting i e coli i o157 h7 i salmonella i spp and i shigella sonnei i |
| topic | polyvalent phage effectiveness <i>Escherichia coli</i> O157:H7 <i>Salmonella</i> <i>Shigella sonnei</i> |
| url | https://www.mdpi.com/2076-2607/9/10/2105 |
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