<i>Veronica persica</i> Ethanol Extract Ameliorates Dinitrochlorobenzene-Induced Atopic Dermatitis-like Skin Inflammation in Mice, Likely by Inducing Nrf2/HO-1 Signaling
Atopic dermatitis (AD) is chronic allergic contact dermatitis with immune dysregulation. <i>Veronica persica</i> has pharmacological activity that prevents asthmatic inflammation by ameliorating inflammatory cell activation. However, the potential effects of the ethanol extract of <i&...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/6/1267 |
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| author | Ki-Shuk Shim Musun Park Won-Kyung Yang Hanbyeol Lee Seung-Hyung Kim Byung-Kil Choo Sungwook Chae Ho-Kyoung Kim Taesoo Kim Ki-Mo Kim |
| author_facet | Ki-Shuk Shim Musun Park Won-Kyung Yang Hanbyeol Lee Seung-Hyung Kim Byung-Kil Choo Sungwook Chae Ho-Kyoung Kim Taesoo Kim Ki-Mo Kim |
| author_sort | Ki-Shuk Shim |
| collection | DOAJ |
| description | Atopic dermatitis (AD) is chronic allergic contact dermatitis with immune dysregulation. <i>Veronica persica</i> has pharmacological activity that prevents asthmatic inflammation by ameliorating inflammatory cell activation. However, the potential effects of the ethanol extract of <i>V. persica</i> (EEVP) on AD remain elusive. This study evaluated the activity and underlying molecular pathway of EEVP in two AD models: dinitrochlorobenzene (DNCB)-induced mice and interferon (IFN)-γ/tumor necrosis factor (TNF)-α-stimulated human HaCaT keratinocytes. EEVP attenuated the DNCB-induced increase in serum immunoglobulin E and histamine levels, mast cell counts in toluidine-blue-stained dorsal skin, inflammatory cytokine (IFN-γ, interleukin [IL]-4, IL-5, and IL-13) levels in cultured splenocytes, and the mRNA expression of <i>IL6</i>, <i>IL13</i>, <i>IL31 receptor</i>, <i>CCR-3</i>, and <i>TNFα</i> in dorsal tissue. Additionally, EEVP inhibited the IFN-γ/TNF-α-induced mRNA expression of <i>IL6</i>, <i>IL13</i>, and <i>CXCL10</i> in HaCaT cells. Furthermore, EEVP restored the IFN-γ/TNF-α-induced downregulation of heme oxygenase (HO)-1 in HaCaT cells by inducing nuclear factor erythroid 2-related factor 2 (Nrf2) expression. A molecular docking analysis demonstrated that EEVP components have a strong affinity to the Kelch-like ECH-associated protein 1 Kelch domain. In summary, EEVP inhibits inflammatory AD by attenuating immune cell activation and inducing the Nrf2/HO-1 signaling pathway in skin keratinocytes. |
| first_indexed | 2024-03-11T02:49:56Z |
| format | Article |
| id | doaj.art-a0c0a4ea89be49a7879107c467b1fb3e |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-11T02:49:56Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-a0c0a4ea89be49a7879107c467b1fb3e2023-11-18T09:03:48ZengMDPI AGAntioxidants2076-39212023-06-01126126710.3390/antiox12061267<i>Veronica persica</i> Ethanol Extract Ameliorates Dinitrochlorobenzene-Induced Atopic Dermatitis-like Skin Inflammation in Mice, Likely by Inducing Nrf2/HO-1 SignalingKi-Shuk Shim0Musun Park1Won-Kyung Yang2Hanbyeol Lee3Seung-Hyung Kim4Byung-Kil Choo5Sungwook Chae6Ho-Kyoung Kim7Taesoo Kim8Ki-Mo Kim9KM Convergence Research Division, Korea Institute of Oriental Medicine, Yuseong-daero 1672, Yuseong-gu, Daejeon 34054, Republic of KoreaKM Data Division, Korea Institute of Oriental Medicine, Yuseong-daero 1672, Yuseong-gu, Daejeon 34054, Republic of KoreaInstitute of Traditional Medicine and Bioscience, Daejeon University, Daejeon 34520, Republic of KoreaInstitute of Traditional Medicine and Bioscience, Daejeon University, Daejeon 34520, Republic of KoreaInstitute of Traditional Medicine and Bioscience, Daejeon University, Daejeon 34520, Republic of KoreaDepartment of Crop Science & Biotechnology, Jeonbuk National University, Jeonju 54896, Republic of KoreaKM Convergence Research Division, Korea Institute of Oriental Medicine, Yuseong-daero 1672, Yuseong-gu, Daejeon 34054, Republic of KoreaKM Convergence Research Division, Korea Institute of Oriental Medicine, Yuseong-daero 1672, Yuseong-gu, Daejeon 34054, Republic of KoreaKM Convergence Research Division, Korea Institute of Oriental Medicine, Yuseong-daero 1672, Yuseong-gu, Daejeon 34054, Republic of KoreaKM Convergence Research Division, Korea Institute of Oriental Medicine, Yuseong-daero 1672, Yuseong-gu, Daejeon 34054, Republic of KoreaAtopic dermatitis (AD) is chronic allergic contact dermatitis with immune dysregulation. <i>Veronica persica</i> has pharmacological activity that prevents asthmatic inflammation by ameliorating inflammatory cell activation. However, the potential effects of the ethanol extract of <i>V. persica</i> (EEVP) on AD remain elusive. This study evaluated the activity and underlying molecular pathway of EEVP in two AD models: dinitrochlorobenzene (DNCB)-induced mice and interferon (IFN)-γ/tumor necrosis factor (TNF)-α-stimulated human HaCaT keratinocytes. EEVP attenuated the DNCB-induced increase in serum immunoglobulin E and histamine levels, mast cell counts in toluidine-blue-stained dorsal skin, inflammatory cytokine (IFN-γ, interleukin [IL]-4, IL-5, and IL-13) levels in cultured splenocytes, and the mRNA expression of <i>IL6</i>, <i>IL13</i>, <i>IL31 receptor</i>, <i>CCR-3</i>, and <i>TNFα</i> in dorsal tissue. Additionally, EEVP inhibited the IFN-γ/TNF-α-induced mRNA expression of <i>IL6</i>, <i>IL13</i>, and <i>CXCL10</i> in HaCaT cells. Furthermore, EEVP restored the IFN-γ/TNF-α-induced downregulation of heme oxygenase (HO)-1 in HaCaT cells by inducing nuclear factor erythroid 2-related factor 2 (Nrf2) expression. A molecular docking analysis demonstrated that EEVP components have a strong affinity to the Kelch-like ECH-associated protein 1 Kelch domain. In summary, EEVP inhibits inflammatory AD by attenuating immune cell activation and inducing the Nrf2/HO-1 signaling pathway in skin keratinocytes.https://www.mdpi.com/2076-3921/12/6/1267atopic dermatitis<i>Veronica persica</i>nuclear factor erythroid 2-related factor 2heme oxygenase-1 |
| spellingShingle | Ki-Shuk Shim Musun Park Won-Kyung Yang Hanbyeol Lee Seung-Hyung Kim Byung-Kil Choo Sungwook Chae Ho-Kyoung Kim Taesoo Kim Ki-Mo Kim <i>Veronica persica</i> Ethanol Extract Ameliorates Dinitrochlorobenzene-Induced Atopic Dermatitis-like Skin Inflammation in Mice, Likely by Inducing Nrf2/HO-1 Signaling Antioxidants atopic dermatitis <i>Veronica persica</i> nuclear factor erythroid 2-related factor 2 heme oxygenase-1 |
| title | <i>Veronica persica</i> Ethanol Extract Ameliorates Dinitrochlorobenzene-Induced Atopic Dermatitis-like Skin Inflammation in Mice, Likely by Inducing Nrf2/HO-1 Signaling |
| title_full | <i>Veronica persica</i> Ethanol Extract Ameliorates Dinitrochlorobenzene-Induced Atopic Dermatitis-like Skin Inflammation in Mice, Likely by Inducing Nrf2/HO-1 Signaling |
| title_fullStr | <i>Veronica persica</i> Ethanol Extract Ameliorates Dinitrochlorobenzene-Induced Atopic Dermatitis-like Skin Inflammation in Mice, Likely by Inducing Nrf2/HO-1 Signaling |
| title_full_unstemmed | <i>Veronica persica</i> Ethanol Extract Ameliorates Dinitrochlorobenzene-Induced Atopic Dermatitis-like Skin Inflammation in Mice, Likely by Inducing Nrf2/HO-1 Signaling |
| title_short | <i>Veronica persica</i> Ethanol Extract Ameliorates Dinitrochlorobenzene-Induced Atopic Dermatitis-like Skin Inflammation in Mice, Likely by Inducing Nrf2/HO-1 Signaling |
| title_sort | i veronica persica i ethanol extract ameliorates dinitrochlorobenzene induced atopic dermatitis like skin inflammation in mice likely by inducing nrf2 ho 1 signaling |
| topic | atopic dermatitis <i>Veronica persica</i> nuclear factor erythroid 2-related factor 2 heme oxygenase-1 |
| url | https://www.mdpi.com/2076-3921/12/6/1267 |
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