Seminal Plasma Lipidomics Profiling to Identify Signatures of Kallmann Syndrome
BackgroundKallmann syndrome (KS) is a rare developmental disorder. Our previous metabolomics work showed substantial changes in linoleic acid and glycerophospholipid metabolism in KS. Here, we performed targeted lipidomics to further identify the differential lipid species in KS.MethodsTwenty-one pa...
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| Format: | Article |
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Frontiers Media S.A.
2021-07-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2021.692690/full |
| _version_ | 1818652214773678080 |
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| author | Xiaogang Li Xiaogang Li Xi Wang Haolong Li Yongzhe Li Ye Guo |
| author_facet | Xiaogang Li Xiaogang Li Xi Wang Haolong Li Yongzhe Li Ye Guo |
| author_sort | Xiaogang Li |
| collection | DOAJ |
| description | BackgroundKallmann syndrome (KS) is a rare developmental disorder. Our previous metabolomics work showed substantial changes in linoleic acid and glycerophospholipid metabolism in KS. Here, we performed targeted lipidomics to further identify the differential lipid species in KS.MethodsTwenty-one patients with KS (treatment group) and twenty-two age-matched healthy controls (HC, control group) were enrolled. Seminal plasma samples and medical records were collected. Targeted lipidomics analysis of these samples was performed using ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS).ResultsLipidomics profiling of patients with KS and the HCs showed clear separation in the orthogonal projections to latent structures-discriminant analysis (OPLS-DA). There were many differential lipids identified, with the main differential lipid species being triacylglycerols (TAGs), phosphatidylcholines (PCs) and phosphatidylethanolamine (PE).ConclusionsThe lipidomics profile of patients with KS changed. It was also determined that TAGs, PCs and PE are promising biomarkers for KS diagnosis. To our knowledge, this is the first report to analyze lipidomics in men with Kallmann syndrome. |
| first_indexed | 2024-12-17T02:18:27Z |
| format | Article |
| id | doaj.art-a0c0c63bd068455dad2f31b65836b66a |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-12-17T02:18:27Z |
| publishDate | 2021-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-a0c0c63bd068455dad2f31b65836b66a2022-12-21T22:07:19ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922021-07-011210.3389/fendo.2021.692690692690Seminal Plasma Lipidomics Profiling to Identify Signatures of Kallmann SyndromeXiaogang Li0Xiaogang Li1Xi Wang2Haolong Li3Yongzhe Li4Ye Guo5Department of Clinical Laboratory, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, ChinaMedical Science Research Center, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, ChinaNational Health Commission (NHC), Key Laboratory of Endocrinology (Peking Union Medical College Hospital), Department of Endocrinology, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, ChinaDepartment of Clinical Laboratory, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, ChinaDepartment of Clinical Laboratory, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, ChinaDepartment of Clinical Laboratory, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, ChinaBackgroundKallmann syndrome (KS) is a rare developmental disorder. Our previous metabolomics work showed substantial changes in linoleic acid and glycerophospholipid metabolism in KS. Here, we performed targeted lipidomics to further identify the differential lipid species in KS.MethodsTwenty-one patients with KS (treatment group) and twenty-two age-matched healthy controls (HC, control group) were enrolled. Seminal plasma samples and medical records were collected. Targeted lipidomics analysis of these samples was performed using ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS).ResultsLipidomics profiling of patients with KS and the HCs showed clear separation in the orthogonal projections to latent structures-discriminant analysis (OPLS-DA). There were many differential lipids identified, with the main differential lipid species being triacylglycerols (TAGs), phosphatidylcholines (PCs) and phosphatidylethanolamine (PE).ConclusionsThe lipidomics profile of patients with KS changed. It was also determined that TAGs, PCs and PE are promising biomarkers for KS diagnosis. To our knowledge, this is the first report to analyze lipidomics in men with Kallmann syndrome.https://www.frontiersin.org/articles/10.3389/fendo.2021.692690/fulltargeted lipidomicsKallmann syndromebiomarkerrare diseaseUPLC-MS/MS |
| spellingShingle | Xiaogang Li Xiaogang Li Xi Wang Haolong Li Yongzhe Li Ye Guo Seminal Plasma Lipidomics Profiling to Identify Signatures of Kallmann Syndrome Frontiers in Endocrinology targeted lipidomics Kallmann syndrome biomarker rare disease UPLC-MS/MS |
| title | Seminal Plasma Lipidomics Profiling to Identify Signatures of Kallmann Syndrome |
| title_full | Seminal Plasma Lipidomics Profiling to Identify Signatures of Kallmann Syndrome |
| title_fullStr | Seminal Plasma Lipidomics Profiling to Identify Signatures of Kallmann Syndrome |
| title_full_unstemmed | Seminal Plasma Lipidomics Profiling to Identify Signatures of Kallmann Syndrome |
| title_short | Seminal Plasma Lipidomics Profiling to Identify Signatures of Kallmann Syndrome |
| title_sort | seminal plasma lipidomics profiling to identify signatures of kallmann syndrome |
| topic | targeted lipidomics Kallmann syndrome biomarker rare disease UPLC-MS/MS |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2021.692690/full |
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