Immunotargeting of collagenase on thrombus
Yi-ling Lu, Ben-xia Yang, Zong-ning YinKey Laboratory of Drug Targeting and Delivery System, West China School of Pharmacy, Sichuan University, Chengdu, Sichuan, People’s Republic of ChinaAbstract: In this study, we aimed to develop a thrombus-targeting delivery system of collagenase b...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2010-11-01
|
| Series: | International Journal of Nanomedicine |
| Online Access: | http://www.dovepress.com/immunotargeting-of-collagenase-on-thrombus-a5654 |
| _version_ | 1818405767793868800 |
|---|---|
| author | Yi-ling Lu Ben-xia Yang Zong-ning Yin |
| author_facet | Yi-ling Lu Ben-xia Yang Zong-ning Yin |
| author_sort | Yi-ling Lu |
| collection | DOAJ |
| description | Yi-ling Lu, Ben-xia Yang, Zong-ning YinKey Laboratory of Drug Targeting and Delivery System, West China School of Pharmacy, Sichuan University, Chengdu, Sichuan, People’s Republic of ChinaAbstract: In this study, we aimed to develop a thrombus-targeting delivery system of collagenase bound to a monoclonal antibody, and to investigate the thrombolysis of an immune-conjugate in vitro and in vivo as well as the targeting effect. We prepared the immunizing conjugation of collagenase by the 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDCI) method. In order to conjugate collagenase and a monoclonal antibody, bovine serum albumin was used as a linker, increasing the number of collagenase molecules carried and keeping collagenase and the monoclonal antibody active. In vitro thrombolysis experiments showed that collagenase had a strong dissolving effect on collagen-embolus within 24 hours. We established a rabbit pulmonary embolism model to investigate the thrombolysis effect of collagenase and collagenase immunizing conjugation in vivo. Our results revealed a significant difference between collagenase and collagenase immunizing conjugation (P < 0.05). We also established a rabbit ear edge vein model to investigate the active target of collagenase immunizing conjugation. We found that collagenase immunizing conjugation had active targets, and had a strong ability to dissolve organized thrombi. In conclusion, the thrombus-targeting delivery system of collagenase we developed has active targeting effects on thrombi.Keywords: thrombus-targeting, collagenase, monoclonal antibody |
| first_indexed | 2024-12-14T09:01:17Z |
| format | Article |
| id | doaj.art-a0c1887061be4037ad73766f0232544d |
| institution | Directory Open Access Journal |
| issn | 1176-9114 1178-2013 |
| language | English |
| last_indexed | 2024-12-14T09:01:17Z |
| publishDate | 2010-11-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-a0c1887061be4037ad73766f0232544d2022-12-21T23:08:48ZengDove Medical PressInternational Journal of Nanomedicine1176-91141178-20132010-11-012010default973982Immunotargeting of collagenase on thrombusYi-ling LuBen-xia YangZong-ning YinYi-ling Lu, Ben-xia Yang, Zong-ning YinKey Laboratory of Drug Targeting and Delivery System, West China School of Pharmacy, Sichuan University, Chengdu, Sichuan, People’s Republic of ChinaAbstract: In this study, we aimed to develop a thrombus-targeting delivery system of collagenase bound to a monoclonal antibody, and to investigate the thrombolysis of an immune-conjugate in vitro and in vivo as well as the targeting effect. We prepared the immunizing conjugation of collagenase by the 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDCI) method. In order to conjugate collagenase and a monoclonal antibody, bovine serum albumin was used as a linker, increasing the number of collagenase molecules carried and keeping collagenase and the monoclonal antibody active. In vitro thrombolysis experiments showed that collagenase had a strong dissolving effect on collagen-embolus within 24 hours. We established a rabbit pulmonary embolism model to investigate the thrombolysis effect of collagenase and collagenase immunizing conjugation in vivo. Our results revealed a significant difference between collagenase and collagenase immunizing conjugation (P < 0.05). We also established a rabbit ear edge vein model to investigate the active target of collagenase immunizing conjugation. We found that collagenase immunizing conjugation had active targets, and had a strong ability to dissolve organized thrombi. In conclusion, the thrombus-targeting delivery system of collagenase we developed has active targeting effects on thrombi.Keywords: thrombus-targeting, collagenase, monoclonal antibodyhttp://www.dovepress.com/immunotargeting-of-collagenase-on-thrombus-a5654 |
| spellingShingle | Yi-ling Lu Ben-xia Yang Zong-ning Yin Immunotargeting of collagenase on thrombus International Journal of Nanomedicine |
| title | Immunotargeting of collagenase on thrombus |
| title_full | Immunotargeting of collagenase on thrombus |
| title_fullStr | Immunotargeting of collagenase on thrombus |
| title_full_unstemmed | Immunotargeting of collagenase on thrombus |
| title_short | Immunotargeting of collagenase on thrombus |
| title_sort | immunotargeting of collagenase on thrombus |
| url | http://www.dovepress.com/immunotargeting-of-collagenase-on-thrombus-a5654 |
| work_keys_str_mv | AT yilinglu immunotargetingofcollagenaseonthrombus AT benxiayang immunotargetingofcollagenaseonthrombus AT zongningyin immunotargetingofcollagenaseonthrombus |