Baicalin alleviates lipopolysaccharide-induced neuroglial activation and inflammatory factors activation in hippocampus of adult mice
Abstract Baicalin is a natural flavonoid that exerts a variety of pharmaceutical effects such as anti-inflammatory and antioxidant. Lipopolysaccharide (LPS) is an endotoxin that releases inflammatory cytokines and induces inflammatory response. This study was investigated the anti-inflammatory mecha...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-09-01
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| Series: | Laboratory Animal Research |
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| Online Access: | http://link.springer.com/article/10.1186/s42826-020-00058-w |
| _version_ | 1818350743493541888 |
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| author | Murad-Ali Shah Dong-Ju Park Ju-Bin Kang Myeong-Ok Kim Phil-Ok Koh |
| author_facet | Murad-Ali Shah Dong-Ju Park Ju-Bin Kang Myeong-Ok Kim Phil-Ok Koh |
| author_sort | Murad-Ali Shah |
| collection | DOAJ |
| description | Abstract Baicalin is a natural flavonoid that exerts a variety of pharmaceutical effects such as anti-inflammatory and antioxidant. Lipopolysaccharide (LPS) is an endotoxin that releases inflammatory cytokines and induces inflammatory response. This study was investigated the anti-inflammatory mechanism of baicalin against LPS-induced inflammatory response in the hippocampus. Adult mice were randomly grouped into control, LPS-treated, and LPS and baicalin co-treated animals. LPS (250 μg/kg/day) and baicalin (10 mg/kg/day) were administered intraperitoneally for 7 consecutive days. We measured neuroglia cells activation and inflammatory factors activation using Western blot analysis and immunofluorescence staining techniques. Ionized calcium binding adaptor molecule-1 (Iba-1) and glial fibrillary acidic protein (GFAP) are widely used as microglia and astrocyte markers, respectively. LPS treatment increased Iba-1 and GFAP expression, while baicalin co-treatment attenuated this overexpression. Nuclear factor-kappa B (NF-κB) is a key mediator of inflammation. Baicalin co-treatment alleviated LPS-induced increase of NF-κB in the hippocampus. In addition, LPS treatment upregulated pro-inflammatory cytokines including interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). However, baicalin co-treatment prevented LPS-induced increases of IL-1β and TNF-α in the hippocampus. Results from the present study showed that baicalin suppresses LPS-induced neuroinflammation by regulating microglia and astrocyte activation and modulating inflammatory factors in the hippocampus. Thus, these results demonstrate that baicalin has neuroprotective effect by alleviates microglia and astrocyte activation and modulates inflammatory response by suppressing NF-κB expression in hippocampus with neuroinflammation caused by LPS. |
| first_indexed | 2024-12-13T18:26:42Z |
| format | Article |
| id | doaj.art-a0c3ae1396894898b451da1796859e20 |
| institution | Directory Open Access Journal |
| issn | 2233-7660 |
| language | English |
| last_indexed | 2024-12-13T18:26:42Z |
| publishDate | 2020-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Laboratory Animal Research |
| spelling | doaj.art-a0c3ae1396894898b451da1796859e202022-12-21T23:35:36ZengBMCLaboratory Animal Research2233-76602020-09-0136111110.1186/s42826-020-00058-wBaicalin alleviates lipopolysaccharide-induced neuroglial activation and inflammatory factors activation in hippocampus of adult miceMurad-Ali Shah0Dong-Ju Park1Ju-Bin Kang2Myeong-Ok Kim3Phil-Ok Koh4Department of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National UniversityDepartment of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National UniversityDepartment of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National UniversityDivision of Life Science and Applied Life Science, College of Natural Sciences, Gyeongsang National UniversityDepartment of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National UniversityAbstract Baicalin is a natural flavonoid that exerts a variety of pharmaceutical effects such as anti-inflammatory and antioxidant. Lipopolysaccharide (LPS) is an endotoxin that releases inflammatory cytokines and induces inflammatory response. This study was investigated the anti-inflammatory mechanism of baicalin against LPS-induced inflammatory response in the hippocampus. Adult mice were randomly grouped into control, LPS-treated, and LPS and baicalin co-treated animals. LPS (250 μg/kg/day) and baicalin (10 mg/kg/day) were administered intraperitoneally for 7 consecutive days. We measured neuroglia cells activation and inflammatory factors activation using Western blot analysis and immunofluorescence staining techniques. Ionized calcium binding adaptor molecule-1 (Iba-1) and glial fibrillary acidic protein (GFAP) are widely used as microglia and astrocyte markers, respectively. LPS treatment increased Iba-1 and GFAP expression, while baicalin co-treatment attenuated this overexpression. Nuclear factor-kappa B (NF-κB) is a key mediator of inflammation. Baicalin co-treatment alleviated LPS-induced increase of NF-κB in the hippocampus. In addition, LPS treatment upregulated pro-inflammatory cytokines including interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). However, baicalin co-treatment prevented LPS-induced increases of IL-1β and TNF-α in the hippocampus. Results from the present study showed that baicalin suppresses LPS-induced neuroinflammation by regulating microglia and astrocyte activation and modulating inflammatory factors in the hippocampus. Thus, these results demonstrate that baicalin has neuroprotective effect by alleviates microglia and astrocyte activation and modulates inflammatory response by suppressing NF-κB expression in hippocampus with neuroinflammation caused by LPS.http://link.springer.com/article/10.1186/s42826-020-00058-wBaicalinHippocampusLipopolysaccharideNeuroinflammation |
| spellingShingle | Murad-Ali Shah Dong-Ju Park Ju-Bin Kang Myeong-Ok Kim Phil-Ok Koh Baicalin alleviates lipopolysaccharide-induced neuroglial activation and inflammatory factors activation in hippocampus of adult mice Laboratory Animal Research Baicalin Hippocampus Lipopolysaccharide Neuroinflammation |
| title | Baicalin alleviates lipopolysaccharide-induced neuroglial activation and inflammatory factors activation in hippocampus of adult mice |
| title_full | Baicalin alleviates lipopolysaccharide-induced neuroglial activation and inflammatory factors activation in hippocampus of adult mice |
| title_fullStr | Baicalin alleviates lipopolysaccharide-induced neuroglial activation and inflammatory factors activation in hippocampus of adult mice |
| title_full_unstemmed | Baicalin alleviates lipopolysaccharide-induced neuroglial activation and inflammatory factors activation in hippocampus of adult mice |
| title_short | Baicalin alleviates lipopolysaccharide-induced neuroglial activation and inflammatory factors activation in hippocampus of adult mice |
| title_sort | baicalin alleviates lipopolysaccharide induced neuroglial activation and inflammatory factors activation in hippocampus of adult mice |
| topic | Baicalin Hippocampus Lipopolysaccharide Neuroinflammation |
| url | http://link.springer.com/article/10.1186/s42826-020-00058-w |
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