Early stage evaluation of cancer stem cells using platinum nanoparticles/CD133 + enhanced nanobiocomposite
Abstract Background Cancer stem cells (CSCs) are of great diagnostic importance due to their involvement in tumorigenesis, therapeutic resistance, metastasis and relapse. Method In this work, a sensitive electrochemical cytosensor was successfully established to detect HT-29 colorectal cancer stem c...
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| Format: | Article |
| Language: | English |
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BMC
2023-05-01
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| Series: | Cancer Nanotechnology |
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| Online Access: | https://doi.org/10.1186/s12645-023-00208-4 |
| _version_ | 1797823029364916224 |
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| author | Solmaz Sadi Balal Khalilzadeh Mahdi Mahdipour Fatemeh Sokouti Nasimi Ibrahim Isildak Soodabeh Davaran Mohammad-Reza Rashidi Farhad Bani |
| author_facet | Solmaz Sadi Balal Khalilzadeh Mahdi Mahdipour Fatemeh Sokouti Nasimi Ibrahim Isildak Soodabeh Davaran Mohammad-Reza Rashidi Farhad Bani |
| author_sort | Solmaz Sadi |
| collection | DOAJ |
| description | Abstract Background Cancer stem cells (CSCs) are of great diagnostic importance due to their involvement in tumorigenesis, therapeutic resistance, metastasis and relapse. Method In this work, a sensitive electrochemical cytosensor was successfully established to detect HT-29 colorectal cancer stem cells based on a nanocomposite composed of mesoporous silica nanoparticles (MSNs) and platinum nanoparticles (PtNPs) using a simple and fast electrodeposition technique on a glassy carbon electrode (GCE). Results According to SEM images, the PtNPs nanoparticles formed on the MSNs substrate are about 100 nm. As expected, high-rate porosity, increased surface-to-volume ratio, provides appropriate local electron transfer rate and suitable platform for the efficient formation of PtNPs. These features allow direct and stable binding of biotinylated monoclonal antibody of CD133 to streptavidin (Strep) and the subsequent availability of active sites for CSCs identification. Differential pulse voltammetry (DPV) results show that close interaction of CD133 + cells with monoclonal antibodies reduces charge transfer and electrical current, as confirmed by square wave voltammogram (SWV). Based on the recorded current versus number of CSCs, we noted that our developed system can sense CSCs from 5 to 20 cells/5 μL. Conclusions As a proof of concept, the designed nanobiocomposite was able to specifically detect CD133 + cells compared to whole HT-29 cells before magnetic activated cell sorting (MACS) process. |
| first_indexed | 2024-03-13T10:17:33Z |
| format | Article |
| id | doaj.art-a0c53decfbc240a99ec4396991887cd3 |
| institution | Directory Open Access Journal |
| issn | 1868-6958 1868-6966 |
| language | English |
| last_indexed | 2024-03-13T10:17:33Z |
| publishDate | 2023-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Nanotechnology |
| spelling | doaj.art-a0c53decfbc240a99ec4396991887cd32023-05-21T11:08:45ZengBMCCancer Nanotechnology1868-69581868-69662023-05-0114111510.1186/s12645-023-00208-4Early stage evaluation of cancer stem cells using platinum nanoparticles/CD133 + enhanced nanobiocompositeSolmaz Sadi0Balal Khalilzadeh1Mahdi Mahdipour2Fatemeh Sokouti Nasimi3Ibrahim Isildak4Soodabeh Davaran5Mohammad-Reza Rashidi6Farhad Bani7Department of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical SciencesStem Cell Research Center, Tabriz University of Medical SciencesStem Cell Research Center, Tabriz University of Medical SciencesStem Cell Research Center, Tabriz University of Medical SciencesDepartment of Bioengineering, Faculty of Chemistry-Metallurgy, Yildiz Technical UniversityDepartment of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical SciencesDepartment of Medicinal Chemistry, Faculty of Pharmacy, Tabriz University of Medical SciencesDepartment of Medical Nanotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical SciencesAbstract Background Cancer stem cells (CSCs) are of great diagnostic importance due to their involvement in tumorigenesis, therapeutic resistance, metastasis and relapse. Method In this work, a sensitive electrochemical cytosensor was successfully established to detect HT-29 colorectal cancer stem cells based on a nanocomposite composed of mesoporous silica nanoparticles (MSNs) and platinum nanoparticles (PtNPs) using a simple and fast electrodeposition technique on a glassy carbon electrode (GCE). Results According to SEM images, the PtNPs nanoparticles formed on the MSNs substrate are about 100 nm. As expected, high-rate porosity, increased surface-to-volume ratio, provides appropriate local electron transfer rate and suitable platform for the efficient formation of PtNPs. These features allow direct and stable binding of biotinylated monoclonal antibody of CD133 to streptavidin (Strep) and the subsequent availability of active sites for CSCs identification. Differential pulse voltammetry (DPV) results show that close interaction of CD133 + cells with monoclonal antibodies reduces charge transfer and electrical current, as confirmed by square wave voltammogram (SWV). Based on the recorded current versus number of CSCs, we noted that our developed system can sense CSCs from 5 to 20 cells/5 μL. Conclusions As a proof of concept, the designed nanobiocomposite was able to specifically detect CD133 + cells compared to whole HT-29 cells before magnetic activated cell sorting (MACS) process.https://doi.org/10.1186/s12645-023-00208-4Cancer stem cellsCD133Platinum nanoparticlesSilica nanocompositeBiosensorColorectal cancer |
| spellingShingle | Solmaz Sadi Balal Khalilzadeh Mahdi Mahdipour Fatemeh Sokouti Nasimi Ibrahim Isildak Soodabeh Davaran Mohammad-Reza Rashidi Farhad Bani Early stage evaluation of cancer stem cells using platinum nanoparticles/CD133 + enhanced nanobiocomposite Cancer Nanotechnology Cancer stem cells CD133 Platinum nanoparticles Silica nanocomposite Biosensor Colorectal cancer |
| title | Early stage evaluation of cancer stem cells using platinum nanoparticles/CD133 + enhanced nanobiocomposite |
| title_full | Early stage evaluation of cancer stem cells using platinum nanoparticles/CD133 + enhanced nanobiocomposite |
| title_fullStr | Early stage evaluation of cancer stem cells using platinum nanoparticles/CD133 + enhanced nanobiocomposite |
| title_full_unstemmed | Early stage evaluation of cancer stem cells using platinum nanoparticles/CD133 + enhanced nanobiocomposite |
| title_short | Early stage evaluation of cancer stem cells using platinum nanoparticles/CD133 + enhanced nanobiocomposite |
| title_sort | early stage evaluation of cancer stem cells using platinum nanoparticles cd133 enhanced nanobiocomposite |
| topic | Cancer stem cells CD133 Platinum nanoparticles Silica nanocomposite Biosensor Colorectal cancer |
| url | https://doi.org/10.1186/s12645-023-00208-4 |
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