Independent S-locus mutations caused self-fertility in Arabidopsis thaliana.
A common yet poorly understood evolutionary transition among flowering plants is a switch from outbreeding to an inbreeding mode of mating. The model plant Arabidopsis thaliana evolved to an inbreeding state through the loss of self-incompatibility, a pollen-rejection system in which pollen recognit...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2009-03-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC2650789?pdf=render |
| _version_ | 1811325486644592640 |
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| author | Nathan A Boggs June B Nasrallah Mikhail E Nasrallah |
| author_facet | Nathan A Boggs June B Nasrallah Mikhail E Nasrallah |
| author_sort | Nathan A Boggs |
| collection | DOAJ |
| description | A common yet poorly understood evolutionary transition among flowering plants is a switch from outbreeding to an inbreeding mode of mating. The model plant Arabidopsis thaliana evolved to an inbreeding state through the loss of self-incompatibility, a pollen-rejection system in which pollen recognition by the stigma is determined by tightly linked and co-evolving alleles of the S-locus receptor kinase (SRK) and its S-locus cysteine-rich ligand (SCR). Transformation of A. thaliana, with a functional AlSRKb-SCRb gene pair from its outcrossing relative A. lyrata, demonstrated that A. thaliana accessions harbor different sets of cryptic self-fertility-promoting mutations, not only in S-locus genes, but also in other loci required for self-incompatibility. However, it is still not known how many times and in what manner the switch to self-fertility occurred in the A. thaliana lineage. Here, we report on our identification of four accessions that are reverted to full self-incompatibility by transformation with AlSRKb-SCRb, bringing to five the number of accessions in which self-fertility is due to, and was likely caused by, S-locus inactivation. Analysis of S-haplotype organization reveals that inter-haplotypic recombination events, rearrangements, and deletions have restructured the S locus and its genes in these accessions. We also perform a Quantitative Trait Loci (QTL) analysis to identify modifier loci associated with self-fertility in the Col-0 reference accession, which cannot be reverted to full self-incompatibility. Our results indicate that the transition to inbreeding occurred by at least two, and possibly more, independent S-locus mutations, and identify a novel unstable modifier locus that contributes to self-fertility in Col-0. |
| first_indexed | 2024-04-13T14:34:01Z |
| format | Article |
| id | doaj.art-a0c5da06b9674b0b840d3b494248aa0d |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-04-13T14:34:01Z |
| publishDate | 2009-03-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-a0c5da06b9674b0b840d3b494248aa0d2022-12-22T02:43:06ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042009-03-0153e100042610.1371/journal.pgen.1000426Independent S-locus mutations caused self-fertility in Arabidopsis thaliana.Nathan A BoggsJune B NasrallahMikhail E NasrallahA common yet poorly understood evolutionary transition among flowering plants is a switch from outbreeding to an inbreeding mode of mating. The model plant Arabidopsis thaliana evolved to an inbreeding state through the loss of self-incompatibility, a pollen-rejection system in which pollen recognition by the stigma is determined by tightly linked and co-evolving alleles of the S-locus receptor kinase (SRK) and its S-locus cysteine-rich ligand (SCR). Transformation of A. thaliana, with a functional AlSRKb-SCRb gene pair from its outcrossing relative A. lyrata, demonstrated that A. thaliana accessions harbor different sets of cryptic self-fertility-promoting mutations, not only in S-locus genes, but also in other loci required for self-incompatibility. However, it is still not known how many times and in what manner the switch to self-fertility occurred in the A. thaliana lineage. Here, we report on our identification of four accessions that are reverted to full self-incompatibility by transformation with AlSRKb-SCRb, bringing to five the number of accessions in which self-fertility is due to, and was likely caused by, S-locus inactivation. Analysis of S-haplotype organization reveals that inter-haplotypic recombination events, rearrangements, and deletions have restructured the S locus and its genes in these accessions. We also perform a Quantitative Trait Loci (QTL) analysis to identify modifier loci associated with self-fertility in the Col-0 reference accession, which cannot be reverted to full self-incompatibility. Our results indicate that the transition to inbreeding occurred by at least two, and possibly more, independent S-locus mutations, and identify a novel unstable modifier locus that contributes to self-fertility in Col-0.http://europepmc.org/articles/PMC2650789?pdf=render |
| spellingShingle | Nathan A Boggs June B Nasrallah Mikhail E Nasrallah Independent S-locus mutations caused self-fertility in Arabidopsis thaliana. PLoS Genetics |
| title | Independent S-locus mutations caused self-fertility in Arabidopsis thaliana. |
| title_full | Independent S-locus mutations caused self-fertility in Arabidopsis thaliana. |
| title_fullStr | Independent S-locus mutations caused self-fertility in Arabidopsis thaliana. |
| title_full_unstemmed | Independent S-locus mutations caused self-fertility in Arabidopsis thaliana. |
| title_short | Independent S-locus mutations caused self-fertility in Arabidopsis thaliana. |
| title_sort | independent s locus mutations caused self fertility in arabidopsis thaliana |
| url | http://europepmc.org/articles/PMC2650789?pdf=render |
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