Organelle Imaging with Terahertz Scattering-Type Scanning Near-Field Microscope
Organelles play core roles in living beings, especially in internal cellular actions, but the hidden information inside the cell is difficult to extract in a label-free manner. In recent years, terahertz (THz) imaging has attracted much attention because of its penetration depth in nonpolar and non-...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-09-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/17/13630 |
| _version_ | 1797582340650696704 |
|---|---|
| author | Jie Huang Jie Wang Linghui Guo Dianxing Wu Shihan Yan Tianying Chang Hongliang Cui |
| author_facet | Jie Huang Jie Wang Linghui Guo Dianxing Wu Shihan Yan Tianying Chang Hongliang Cui |
| author_sort | Jie Huang |
| collection | DOAJ |
| description | Organelles play core roles in living beings, especially in internal cellular actions, but the hidden information inside the cell is difficult to extract in a label-free manner. In recent years, terahertz (THz) imaging has attracted much attention because of its penetration depth in nonpolar and non-metallic materials and label-free, non-invasive and non-ionizing ability to obtain the interior information of bio-samples. However, the low spatial resolution of traditional far-field THz imaging systems and the weak dielectric contrast of biological samples hinder the application of this technology in the biological field. In this paper, we used an advanced THz scattering near-field imaging method for detecting chloroplasts on gold substrate with nano-flatness combined with an image processing method to remove the background noise and successfully obtained the subcellular-grade internal reticular structure from an Arabidopsis chloroplast THz image. In contrast, little inner information could be observed in the tea chloroplast in similar THz images. Further, transmission electron microscopy (TEM) and mass spectroscopy (MS) were also used to detect structural and chemical differences inside the chloroplasts of Arabidopsis and tea plants. The preliminary results suggested that the interspecific different THz information is related to the internal spatial structures of chloroplasts and metabolite differences among species. Therefore, this method could open a new way to study the structure of individual organelles. |
| first_indexed | 2024-03-10T23:20:44Z |
| format | Article |
| id | doaj.art-a0c62d5cd8a04259b8074a45c7de2af4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T23:20:44Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a0c62d5cd8a04259b8074a45c7de2af42023-11-19T08:20:04ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124171363010.3390/ijms241713630Organelle Imaging with Terahertz Scattering-Type Scanning Near-Field MicroscopeJie Huang0Jie Wang1Linghui Guo2Dianxing Wu3Shihan Yan4Tianying Chang5Hongliang Cui6State Key Laboratory of Rice Biology, Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou 310029, ChinaChongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, ChinaNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Wuhan 430070, ChinaState Key Laboratory of Rice Biology, Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou 310029, ChinaChongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, ChinaShenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, ChinaChongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, ChinaOrganelles play core roles in living beings, especially in internal cellular actions, but the hidden information inside the cell is difficult to extract in a label-free manner. In recent years, terahertz (THz) imaging has attracted much attention because of its penetration depth in nonpolar and non-metallic materials and label-free, non-invasive and non-ionizing ability to obtain the interior information of bio-samples. However, the low spatial resolution of traditional far-field THz imaging systems and the weak dielectric contrast of biological samples hinder the application of this technology in the biological field. In this paper, we used an advanced THz scattering near-field imaging method for detecting chloroplasts on gold substrate with nano-flatness combined with an image processing method to remove the background noise and successfully obtained the subcellular-grade internal reticular structure from an Arabidopsis chloroplast THz image. In contrast, little inner information could be observed in the tea chloroplast in similar THz images. Further, transmission electron microscopy (TEM) and mass spectroscopy (MS) were also used to detect structural and chemical differences inside the chloroplasts of Arabidopsis and tea plants. The preliminary results suggested that the interspecific different THz information is related to the internal spatial structures of chloroplasts and metabolite differences among species. Therefore, this method could open a new way to study the structure of individual organelles.https://www.mdpi.com/1422-0067/24/17/13630chloroplastTHz imagings-SNOM<i>Arabidopsis thaliana</i><i>Camellia sinensis</i>flavonoid |
| spellingShingle | Jie Huang Jie Wang Linghui Guo Dianxing Wu Shihan Yan Tianying Chang Hongliang Cui Organelle Imaging with Terahertz Scattering-Type Scanning Near-Field Microscope International Journal of Molecular Sciences chloroplast THz imaging s-SNOM <i>Arabidopsis thaliana</i> <i>Camellia sinensis</i> flavonoid |
| title | Organelle Imaging with Terahertz Scattering-Type Scanning Near-Field Microscope |
| title_full | Organelle Imaging with Terahertz Scattering-Type Scanning Near-Field Microscope |
| title_fullStr | Organelle Imaging with Terahertz Scattering-Type Scanning Near-Field Microscope |
| title_full_unstemmed | Organelle Imaging with Terahertz Scattering-Type Scanning Near-Field Microscope |
| title_short | Organelle Imaging with Terahertz Scattering-Type Scanning Near-Field Microscope |
| title_sort | organelle imaging with terahertz scattering type scanning near field microscope |
| topic | chloroplast THz imaging s-SNOM <i>Arabidopsis thaliana</i> <i>Camellia sinensis</i> flavonoid |
| url | https://www.mdpi.com/1422-0067/24/17/13630 |
| work_keys_str_mv | AT jiehuang organelleimagingwithterahertzscatteringtypescanningnearfieldmicroscope AT jiewang organelleimagingwithterahertzscatteringtypescanningnearfieldmicroscope AT linghuiguo organelleimagingwithterahertzscatteringtypescanningnearfieldmicroscope AT dianxingwu organelleimagingwithterahertzscatteringtypescanningnearfieldmicroscope AT shihanyan organelleimagingwithterahertzscatteringtypescanningnearfieldmicroscope AT tianyingchang organelleimagingwithterahertzscatteringtypescanningnearfieldmicroscope AT hongliangcui organelleimagingwithterahertzscatteringtypescanningnearfieldmicroscope |