Co-staining human PBMCs with fluorescent antibodies and antibody-oligonucleotide conjugates for cell sorting prior to single-cell CITE-Seq

Co-staining human PBMCs with fluorescent antibodies and antibody-oligonucleotide conjugates for cell sorting prior to single-cell CITE-Seq

Summary: The dual interrogation of the transcriptome and proteome with single-cell resolution provides exquisite insights into immune mechanisms in health and disease. Here, we describe a cutting-edge protocol wherein we combine Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-Se...

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Bibliographic Details
Main Authors: Xiaoshan Shi, Gisele V. Baracho, Woodrow E. Lomas, III, Stephanie J. Widmann, Aaron J. Tyznik
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Cell isolation
Single Cell
Flow Cytometry/Mass Cytometry
Immunology
Molecular Biology
Antibody
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721005992

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