Rapid detection of human mastadenovirus species B by recombinase polymerase amplification assay
Abstract Background As an important component of the causative agent of respiratory tract infections, enteric and eye infections, Human mastadenoviruses (HAdVs) species B spread easily in the crowd. In this study, we developed a recombinase polymerase amplification (RPA) assay for rapidly detecting...
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| Format: | Article |
| Language: | English |
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BMC
2019-01-01
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| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12866-018-1365-7 |
| _version_ | 1818875520243204096 |
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| author | Tao Wu Haizhen Wu Kangchen Zhao Chaoyou Hu Yiyue Ge Xiaojuan Zhu Xingchen Zhang Minghao Zhou Fengcai Zhu Lunbiao Cui |
| author_facet | Tao Wu Haizhen Wu Kangchen Zhao Chaoyou Hu Yiyue Ge Xiaojuan Zhu Xingchen Zhang Minghao Zhou Fengcai Zhu Lunbiao Cui |
| author_sort | Tao Wu |
| collection | DOAJ |
| description | Abstract Background As an important component of the causative agent of respiratory tract infections, enteric and eye infections, Human mastadenoviruses (HAdVs) species B spread easily in the crowd. In this study, we developed a recombinase polymerase amplification (RPA) assay for rapidly detecting HAdVs species B which was comprised of two different formats (real-time and lateral-flow device). Results This assay was confirmed to be able to detect 5 different HAdVs species B subtypes (HAdV-B3, HAdV-B7, HAdV-B11, HAdV-B14 and HAdV-B55) without cross-reactions with other subtypes and other respiratory tract pathogens. This RPA assay has not only highly sensitivity with low detection limit of 50 copies per reaction but also short reaction time (< 15 min per detection). Furthermore, the real-time RPA assay has excellent correlation with real-time PCR assay for detection of HAdVs species B presented in clinical samples. Conclusions Thus, the RPA assay developed in this study provides an effective and portable approach for the rapid detection of HAdVs species B. |
| first_indexed | 2024-12-19T13:27:48Z |
| format | Article |
| id | doaj.art-a0c82c85714246d788477696d5994e8c |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-19T13:27:48Z |
| publishDate | 2019-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-a0c82c85714246d788477696d5994e8c2022-12-21T20:19:30ZengBMCBMC Microbiology1471-21802019-01-011911710.1186/s12866-018-1365-7Rapid detection of human mastadenovirus species B by recombinase polymerase amplification assayTao Wu0Haizhen Wu1Kangchen Zhao2Chaoyou Hu3Yiyue Ge4Xiaojuan Zhu5Xingchen Zhang6Minghao Zhou7Fengcai Zhu8Lunbiao Cui9Institute of Pathogenic Microbiology, Key Laboratories of Enteric Pathogenic Microbiology (Ministry of Health), Jiangsu Provincial Center for Disease Control and PreventionKunshan Municipal Center for Disease Control and PreventionInstitute of Pathogenic Microbiology, Key Laboratories of Enteric Pathogenic Microbiology (Ministry of Health), Jiangsu Provincial Center for Disease Control and PreventionKunshan Municipal Center for Disease Control and PreventionInstitute of Pathogenic Microbiology, Key Laboratories of Enteric Pathogenic Microbiology (Ministry of Health), Jiangsu Provincial Center for Disease Control and PreventionInstitute of Pathogenic Microbiology, Key Laboratories of Enteric Pathogenic Microbiology (Ministry of Health), Jiangsu Provincial Center for Disease Control and PreventionKunshan Municipal Center for Disease Control and PreventionInstitute of Pathogenic Microbiology, Key Laboratories of Enteric Pathogenic Microbiology (Ministry of Health), Jiangsu Provincial Center for Disease Control and PreventionInstitute of Pathogenic Microbiology, Key Laboratories of Enteric Pathogenic Microbiology (Ministry of Health), Jiangsu Provincial Center for Disease Control and PreventionInstitute of Pathogenic Microbiology, Key Laboratories of Enteric Pathogenic Microbiology (Ministry of Health), Jiangsu Provincial Center for Disease Control and PreventionAbstract Background As an important component of the causative agent of respiratory tract infections, enteric and eye infections, Human mastadenoviruses (HAdVs) species B spread easily in the crowd. In this study, we developed a recombinase polymerase amplification (RPA) assay for rapidly detecting HAdVs species B which was comprised of two different formats (real-time and lateral-flow device). Results This assay was confirmed to be able to detect 5 different HAdVs species B subtypes (HAdV-B3, HAdV-B7, HAdV-B11, HAdV-B14 and HAdV-B55) without cross-reactions with other subtypes and other respiratory tract pathogens. This RPA assay has not only highly sensitivity with low detection limit of 50 copies per reaction but also short reaction time (< 15 min per detection). Furthermore, the real-time RPA assay has excellent correlation with real-time PCR assay for detection of HAdVs species B presented in clinical samples. Conclusions Thus, the RPA assay developed in this study provides an effective and portable approach for the rapid detection of HAdVs species B.http://link.springer.com/article/10.1186/s12866-018-1365-7Human mastadenovirusesRecombinase polymerase amplificationReal-timeLateral-flow device |
| spellingShingle | Tao Wu Haizhen Wu Kangchen Zhao Chaoyou Hu Yiyue Ge Xiaojuan Zhu Xingchen Zhang Minghao Zhou Fengcai Zhu Lunbiao Cui Rapid detection of human mastadenovirus species B by recombinase polymerase amplification assay BMC Microbiology Human mastadenoviruses Recombinase polymerase amplification Real-time Lateral-flow device |
| title | Rapid detection of human mastadenovirus species B by recombinase polymerase amplification assay |
| title_full | Rapid detection of human mastadenovirus species B by recombinase polymerase amplification assay |
| title_fullStr | Rapid detection of human mastadenovirus species B by recombinase polymerase amplification assay |
| title_full_unstemmed | Rapid detection of human mastadenovirus species B by recombinase polymerase amplification assay |
| title_short | Rapid detection of human mastadenovirus species B by recombinase polymerase amplification assay |
| title_sort | rapid detection of human mastadenovirus species b by recombinase polymerase amplification assay |
| topic | Human mastadenoviruses Recombinase polymerase amplification Real-time Lateral-flow device |
| url | http://link.springer.com/article/10.1186/s12866-018-1365-7 |
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