Application of transcriptome analysis to investigate the effects of long-term low temperature stress on liver function in the tiger puffer (Takifugu rubripes)
The tiger puffer (Takifugu rubripes) is an important economic fish species in northern China. However, it is a warm-temperature species, and low winter temperatures can result in high mortality in aquaculture. Understanding the mechanisms of cold resistance in tiger puffers will thus provide critica...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2022.1069711/full |
| _version_ | 1811322743096868864 |
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| author | Zhifeng Liu Zhifeng Liu Liguang Zhu Liguang Zhu Liguang Zhu Xinan Wang Xinan Wang Shiying Liu Shiying Liu Aijun Ma Aijun Ma Haowen Chang Haowen Chang Haowen Chang Zhibin Sun Zhibin Sun Fei Xu Fei Xu Haichi Zhao Haichi Zhao Haichi Zhao |
| author_facet | Zhifeng Liu Zhifeng Liu Liguang Zhu Liguang Zhu Liguang Zhu Xinan Wang Xinan Wang Shiying Liu Shiying Liu Aijun Ma Aijun Ma Haowen Chang Haowen Chang Haowen Chang Zhibin Sun Zhibin Sun Fei Xu Fei Xu Haichi Zhao Haichi Zhao Haichi Zhao |
| author_sort | Zhifeng Liu |
| collection | DOAJ |
| description | The tiger puffer (Takifugu rubripes) is an important economic fish species in northern China. However, it is a warm-temperature species, and low winter temperatures can result in high mortality in aquaculture. Understanding the mechanisms of cold resistance in tiger puffers will thus provide critical information to help cope with winter cold. In this study, we performed transcriptome analysis of livers from puffer fish kept at different temperatures (18°C, 13°C, and 8°C) to identify the key pathways and genes involved in the response to low-temperature stress. We also detected serum levels of proteases, arginine, and proline to obtain further information on the response to cold adaption. Totals of 51, 942, and 195 differentially expressed genes were identified in the 18°C vs 13°C, 18°C vs 8°C, and 13°C vs 8°C groups, respectively. Pathway analysis showed that significantly enriched pathways were mainly related to digestion, metabolism, and environmental adaptation. Most genes in the pathways related to digestion and metabolism were down-regulated, while most genes in the pathways related to environmental adaptation were up-regulated. Serum levels of proteases were significantly lower in the low-temperature groups (13°C and 8°C) compared with the control group (18°C), while arginine and proline levels were significantly higher in the 8°C group compared with the other two groups. These results suggest that low temperature caused digestive and metabolic disorders, as well as adaptive changes to low temperature in tiger puffers. On this premise, we found that some up-regulated genes in the pancreatic secretion pathway, arginine and proline metabolism pathway, and circadian rhythm pathway played important roles in the survival, growth, and development of tiger puffers under low-temperature stress. The accumulation of arginine and proline can maintain metabolism and circulation and resist cold stress. The circadian rhythm is closely related to digestion and metabolism, which is an adaptive change and plays a positive role in the resistance to low temperature. The results of this study provide new insights and a theoretical basis for the study of cold tolerance in tiger puffers. |
| first_indexed | 2024-04-13T13:40:19Z |
| format | Article |
| id | doaj.art-a0c83a2d113a4ed6a14c21d14fe0206b |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-13T13:40:19Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-a0c83a2d113a4ed6a14c21d14fe0206b2022-12-22T02:44:39ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452022-11-01910.3389/fmars.2022.10697111069711Application of transcriptome analysis to investigate the effects of long-term low temperature stress on liver function in the tiger puffer (Takifugu rubripes)Zhifeng Liu0Zhifeng Liu1Liguang Zhu2Liguang Zhu3Liguang Zhu4Xinan Wang5Xinan Wang6Shiying Liu7Shiying Liu8Aijun Ma9Aijun Ma10Haowen Chang11Haowen Chang12Haowen Chang13Zhibin Sun14Zhibin Sun15Fei Xu16Fei Xu17Haichi Zhao18Haichi Zhao19Haichi Zhao20Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaLaboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaLaboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, ChinaCollege of Fisheries and Life Science, Dalian Ocean University, Dalian, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaLaboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaLaboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaLaboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaLaboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, ChinaCollege of Fisheries and Life Science, Shanghai Ocean University, Ministry of Education, Shanghai, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaLaboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaLaboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaLaboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao, ChinaCollege of Fisheries and Life Science, Shanghai Ocean University, Ministry of Education, Shanghai, ChinaThe tiger puffer (Takifugu rubripes) is an important economic fish species in northern China. However, it is a warm-temperature species, and low winter temperatures can result in high mortality in aquaculture. Understanding the mechanisms of cold resistance in tiger puffers will thus provide critical information to help cope with winter cold. In this study, we performed transcriptome analysis of livers from puffer fish kept at different temperatures (18°C, 13°C, and 8°C) to identify the key pathways and genes involved in the response to low-temperature stress. We also detected serum levels of proteases, arginine, and proline to obtain further information on the response to cold adaption. Totals of 51, 942, and 195 differentially expressed genes were identified in the 18°C vs 13°C, 18°C vs 8°C, and 13°C vs 8°C groups, respectively. Pathway analysis showed that significantly enriched pathways were mainly related to digestion, metabolism, and environmental adaptation. Most genes in the pathways related to digestion and metabolism were down-regulated, while most genes in the pathways related to environmental adaptation were up-regulated. Serum levels of proteases were significantly lower in the low-temperature groups (13°C and 8°C) compared with the control group (18°C), while arginine and proline levels were significantly higher in the 8°C group compared with the other two groups. These results suggest that low temperature caused digestive and metabolic disorders, as well as adaptive changes to low temperature in tiger puffers. On this premise, we found that some up-regulated genes in the pancreatic secretion pathway, arginine and proline metabolism pathway, and circadian rhythm pathway played important roles in the survival, growth, and development of tiger puffers under low-temperature stress. The accumulation of arginine and proline can maintain metabolism and circulation and resist cold stress. The circadian rhythm is closely related to digestion and metabolism, which is an adaptive change and plays a positive role in the resistance to low temperature. The results of this study provide new insights and a theoretical basis for the study of cold tolerance in tiger puffers.https://www.frontiersin.org/articles/10.3389/fmars.2022.1069711/fullTakifugu rubripeslow temperaturelivertranscriptomeqPCR |
| spellingShingle | Zhifeng Liu Zhifeng Liu Liguang Zhu Liguang Zhu Liguang Zhu Xinan Wang Xinan Wang Shiying Liu Shiying Liu Aijun Ma Aijun Ma Haowen Chang Haowen Chang Haowen Chang Zhibin Sun Zhibin Sun Fei Xu Fei Xu Haichi Zhao Haichi Zhao Haichi Zhao Application of transcriptome analysis to investigate the effects of long-term low temperature stress on liver function in the tiger puffer (Takifugu rubripes) Frontiers in Marine Science Takifugu rubripes low temperature liver transcriptome qPCR |
| title | Application of transcriptome analysis to investigate the effects of long-term low temperature stress on liver function in the tiger puffer (Takifugu rubripes) |
| title_full | Application of transcriptome analysis to investigate the effects of long-term low temperature stress on liver function in the tiger puffer (Takifugu rubripes) |
| title_fullStr | Application of transcriptome analysis to investigate the effects of long-term low temperature stress on liver function in the tiger puffer (Takifugu rubripes) |
| title_full_unstemmed | Application of transcriptome analysis to investigate the effects of long-term low temperature stress on liver function in the tiger puffer (Takifugu rubripes) |
| title_short | Application of transcriptome analysis to investigate the effects of long-term low temperature stress on liver function in the tiger puffer (Takifugu rubripes) |
| title_sort | application of transcriptome analysis to investigate the effects of long term low temperature stress on liver function in the tiger puffer takifugu rubripes |
| topic | Takifugu rubripes low temperature liver transcriptome qPCR |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2022.1069711/full |
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