High-throughput bactericidal assays for monoclonal antibody screening against antimicrobial resistant Neisseria gonorrhoeae
Neisseria gonorrhoeae (gonococcus) is an obligate human pathogen and the etiological agent of the sexually transmitted disease gonorrhea. The rapid rise in gonococcal resistance to all currently available antimicrobials has become a significant public health burden and the need to develop novel ther...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1243427/full |
| _version_ | 1797741915147337728 |
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| author | Samuele Stazzoni Marco Troisi Valentina Abbiento Valentina Abbiento Claudia Sala Emanuele Andreano Rino Rappuoli Rino Rappuoli |
| author_facet | Samuele Stazzoni Marco Troisi Valentina Abbiento Valentina Abbiento Claudia Sala Emanuele Andreano Rino Rappuoli Rino Rappuoli |
| author_sort | Samuele Stazzoni |
| collection | DOAJ |
| description | Neisseria gonorrhoeae (gonococcus) is an obligate human pathogen and the etiological agent of the sexually transmitted disease gonorrhea. The rapid rise in gonococcal resistance to all currently available antimicrobials has become a significant public health burden and the need to develop novel therapeutic and prophylactic tools is now a global priority. While high-throughput screening methods allowed rapid discovery of extremely potent monoclonal antibodies (mAbs) against viral pathogens, the field of bacteriology suffers from the lack of assays that allow efficient screening of large panels of samples. To address this point, we developed luminescence-based (L-ABA) and resazurin-based (R-ABA) antibody bactericidal assays that measure N. gonorrhoeae metabolic activity as a proxy of bacterial viability. Both L-ABA and R-ABA are applicable on the large scale for the rapid identification of bactericidal antibodies and were validated by conventional methods. Implementation of these approaches will be instrumental to the development of new medications and vaccines against N. gonorrhoeae and other bacterial pathogens to support the fight against antimicrobial resistance. |
| first_indexed | 2024-03-12T14:34:27Z |
| format | Article |
| id | doaj.art-a0c974db7e94420bb66e994d5f7db92d |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T14:34:27Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-a0c974db7e94420bb66e994d5f7db92d2023-08-17T12:13:36ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-08-011410.3389/fmicb.2023.12434271243427High-throughput bactericidal assays for monoclonal antibody screening against antimicrobial resistant Neisseria gonorrhoeaeSamuele Stazzoni0Marco Troisi1Valentina Abbiento2Valentina Abbiento3Claudia Sala4Emanuele Andreano5Rino Rappuoli6Rino Rappuoli7Monoclonal Antibody Discovery Laboratory, Fondazione Toscana Life Sciences, Siena, ItalyMonoclonal Antibody Discovery Laboratory, Fondazione Toscana Life Sciences, Siena, ItalyMonoclonal Antibody Discovery Laboratory, Fondazione Toscana Life Sciences, Siena, ItalyDepartment of Biotechnology, Chemistry and Pharmacy, University of Siena, Siena, ItalyMonoclonal Antibody Discovery Laboratory, Fondazione Toscana Life Sciences, Siena, ItalyMonoclonal Antibody Discovery Laboratory, Fondazione Toscana Life Sciences, Siena, ItalyDepartment of Biotechnology, Chemistry and Pharmacy, University of Siena, Siena, ItalyFondazione Biotecnopolo di Siena, Siena, ItalyNeisseria gonorrhoeae (gonococcus) is an obligate human pathogen and the etiological agent of the sexually transmitted disease gonorrhea. The rapid rise in gonococcal resistance to all currently available antimicrobials has become a significant public health burden and the need to develop novel therapeutic and prophylactic tools is now a global priority. While high-throughput screening methods allowed rapid discovery of extremely potent monoclonal antibodies (mAbs) against viral pathogens, the field of bacteriology suffers from the lack of assays that allow efficient screening of large panels of samples. To address this point, we developed luminescence-based (L-ABA) and resazurin-based (R-ABA) antibody bactericidal assays that measure N. gonorrhoeae metabolic activity as a proxy of bacterial viability. Both L-ABA and R-ABA are applicable on the large scale for the rapid identification of bactericidal antibodies and were validated by conventional methods. Implementation of these approaches will be instrumental to the development of new medications and vaccines against N. gonorrhoeae and other bacterial pathogens to support the fight against antimicrobial resistance.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1243427/fullmonoclonal antibodiesNeisseria gonorrhoeaebacteriaantimicrobial resistancetherapeuticshigh-throughput screening |
| spellingShingle | Samuele Stazzoni Marco Troisi Valentina Abbiento Valentina Abbiento Claudia Sala Emanuele Andreano Rino Rappuoli Rino Rappuoli High-throughput bactericidal assays for monoclonal antibody screening against antimicrobial resistant Neisseria gonorrhoeae Frontiers in Microbiology monoclonal antibodies Neisseria gonorrhoeae bacteria antimicrobial resistance therapeutics high-throughput screening |
| title | High-throughput bactericidal assays for monoclonal antibody screening against antimicrobial resistant Neisseria gonorrhoeae |
| title_full | High-throughput bactericidal assays for monoclonal antibody screening against antimicrobial resistant Neisseria gonorrhoeae |
| title_fullStr | High-throughput bactericidal assays for monoclonal antibody screening against antimicrobial resistant Neisseria gonorrhoeae |
| title_full_unstemmed | High-throughput bactericidal assays for monoclonal antibody screening against antimicrobial resistant Neisseria gonorrhoeae |
| title_short | High-throughput bactericidal assays for monoclonal antibody screening against antimicrobial resistant Neisseria gonorrhoeae |
| title_sort | high throughput bactericidal assays for monoclonal antibody screening against antimicrobial resistant neisseria gonorrhoeae |
| topic | monoclonal antibodies Neisseria gonorrhoeae bacteria antimicrobial resistance therapeutics high-throughput screening |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1243427/full |
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