Overexpression of lncRNA‐NEF regulates the miR‐155/PTEN axis to inhibit adipogenesis and promote osteogenesis
Abstract Osteoporosis is characterized by osteopenia and bone tissue microstructure degradation. Adipose‐derived stem cells (ADSCs) are multipotent adult stem cells that have the ability to yield mesenchymal stem cells and have the potential to undergo osteogenesis and bone regeneration. Therefore,...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-11-01
|
| Series: | Kaohsiung Journal of Medical Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/kjm2.12423 |
| _version_ | 1818928539451260928 |
|---|---|
| author | Ying Ming Zheng‐Peng Liu |
| author_facet | Ying Ming Zheng‐Peng Liu |
| author_sort | Ying Ming |
| collection | DOAJ |
| description | Abstract Osteoporosis is characterized by osteopenia and bone tissue microstructure degradation. Adipose‐derived stem cells (ADSCs) are multipotent adult stem cells that have the ability to yield mesenchymal stem cells and have the potential to undergo osteogenesis and bone regeneration. Therefore, ADSCs have the potential to treat osteoporosis, but the molecular mechanism of these cells in the process of osteogenesis and osteoclasts is still not clear. In the present study, we collected serum samples from 10 clinical osteoporosis patients to detect long noncoding RNA‐neighboring enhancer of FOXA2 (lncRNA‐NEF) and miR‐155 expression levels. Half of these patients were senile and half were postmenopausal women, and nine of them have used steroids for a long time, in which ADSCs were cultured and induced to adipogenic and osteogenic differentiations. Quantitative real‐time polymerase chain reaction was used to detect the expression of genes in ADSCs. Overexpression of lncRNA‐NEF in ADSCs were undertaken to verify its regulatory function on cell osteogenic and adipogenic differentiations. A luciferase activity experiment was performed to determine the relationship between miR‐155 and phosphatase and tensin homologue deleted on chromosome 10 (PTEN). The level of lncRNA‐NEF was downregulated, and miR‐155 was upregulated, in serum samples from patients with clinical osteoporosis. LncRNA‐NEF showed different expression levels in the induction of osteogenic or adipogenic differentiation, which increased during osteogenic induction and decreased during adipogenic induction. Overexpression of lncRNA‐NEF or downregulation of miR‐155 in ADSCs promoted osteogenic differentiation and inhibited adipogenesis progression. PTEN was the direct target of miR‐155 and was involved in the regulation of osteogenic differentiation. Overexpression of lncRNA‐NEF regulated the miR‐155/PTEN axis to inhibit adipogenesis and promote osteogenesis in ADSCs. |
| first_indexed | 2024-12-20T03:30:31Z |
| format | Article |
| id | doaj.art-a0cb710da7ea43f0928c6e7ad3a26c82 |
| institution | Directory Open Access Journal |
| issn | 1607-551X 2410-8650 |
| language | English |
| last_indexed | 2024-12-20T03:30:31Z |
| publishDate | 2021-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Kaohsiung Journal of Medical Sciences |
| spelling | doaj.art-a0cb710da7ea43f0928c6e7ad3a26c822022-12-21T19:54:59ZengWileyKaohsiung Journal of Medical Sciences1607-551X2410-86502021-11-01371193093910.1002/kjm2.12423Overexpression of lncRNA‐NEF regulates the miR‐155/PTEN axis to inhibit adipogenesis and promote osteogenesisYing Ming0Zheng‐Peng Liu1Department of Clinical Laboratory Affiliated Hospital of Chengde Medical University Chengde Hebei Province ChinaDepartment of Spinal Surgery Ward Affiliated Hospital of Chengde Medical University Chengde Hebei Province ChinaAbstract Osteoporosis is characterized by osteopenia and bone tissue microstructure degradation. Adipose‐derived stem cells (ADSCs) are multipotent adult stem cells that have the ability to yield mesenchymal stem cells and have the potential to undergo osteogenesis and bone regeneration. Therefore, ADSCs have the potential to treat osteoporosis, but the molecular mechanism of these cells in the process of osteogenesis and osteoclasts is still not clear. In the present study, we collected serum samples from 10 clinical osteoporosis patients to detect long noncoding RNA‐neighboring enhancer of FOXA2 (lncRNA‐NEF) and miR‐155 expression levels. Half of these patients were senile and half were postmenopausal women, and nine of them have used steroids for a long time, in which ADSCs were cultured and induced to adipogenic and osteogenic differentiations. Quantitative real‐time polymerase chain reaction was used to detect the expression of genes in ADSCs. Overexpression of lncRNA‐NEF in ADSCs were undertaken to verify its regulatory function on cell osteogenic and adipogenic differentiations. A luciferase activity experiment was performed to determine the relationship between miR‐155 and phosphatase and tensin homologue deleted on chromosome 10 (PTEN). The level of lncRNA‐NEF was downregulated, and miR‐155 was upregulated, in serum samples from patients with clinical osteoporosis. LncRNA‐NEF showed different expression levels in the induction of osteogenic or adipogenic differentiation, which increased during osteogenic induction and decreased during adipogenic induction. Overexpression of lncRNA‐NEF or downregulation of miR‐155 in ADSCs promoted osteogenic differentiation and inhibited adipogenesis progression. PTEN was the direct target of miR‐155 and was involved in the regulation of osteogenic differentiation. Overexpression of lncRNA‐NEF regulated the miR‐155/PTEN axis to inhibit adipogenesis and promote osteogenesis in ADSCs.https://doi.org/10.1002/kjm2.12423adipogenesislncRNA‐NEFmiR‐155osteogenesisPTEN |
| spellingShingle | Ying Ming Zheng‐Peng Liu Overexpression of lncRNA‐NEF regulates the miR‐155/PTEN axis to inhibit adipogenesis and promote osteogenesis Kaohsiung Journal of Medical Sciences adipogenesis lncRNA‐NEF miR‐155 osteogenesis PTEN |
| title | Overexpression of lncRNA‐NEF regulates the miR‐155/PTEN axis to inhibit adipogenesis and promote osteogenesis |
| title_full | Overexpression of lncRNA‐NEF regulates the miR‐155/PTEN axis to inhibit adipogenesis and promote osteogenesis |
| title_fullStr | Overexpression of lncRNA‐NEF regulates the miR‐155/PTEN axis to inhibit adipogenesis and promote osteogenesis |
| title_full_unstemmed | Overexpression of lncRNA‐NEF regulates the miR‐155/PTEN axis to inhibit adipogenesis and promote osteogenesis |
| title_short | Overexpression of lncRNA‐NEF regulates the miR‐155/PTEN axis to inhibit adipogenesis and promote osteogenesis |
| title_sort | overexpression of lncrna nef regulates the mir 155 pten axis to inhibit adipogenesis and promote osteogenesis |
| topic | adipogenesis lncRNA‐NEF miR‐155 osteogenesis PTEN |
| url | https://doi.org/10.1002/kjm2.12423 |
| work_keys_str_mv | AT yingming overexpressionoflncrnanefregulatesthemir155ptenaxistoinhibitadipogenesisandpromoteosteogenesis AT zhengpengliu overexpressionoflncrnanefregulatesthemir155ptenaxistoinhibitadipogenesisandpromoteosteogenesis |