Selection of Specific Aptamer against Rivaroxaban and Utilization for Label-Free Electrochemical Aptasensing Using Gold Nanoparticles: First Announcement and Application for Clinical Sample Analysis
For the first time, a novel aptamer was designed and utilized for the selective detection of rivaroxaban (RIV) using the integration of bioinformatics with biosensing technology. The selected aptamer with the sequence 5′-TAG GGA AGA GAA GGA CAT ATG ATG ACT CAC AAC TGG ACG AAC GTA CTT ATC CCC CCC AAT...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/10/773 |
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| author | Rokhsareh Ebrahimi Abolfazl Barzegari Reza Teimuri-Mofrad Houman Kholafazad Kordasht Mohammad Hasanzadeh Maryam Khoubnasabjafari Vahid Jouyban-Gharamaleki Abbas Afrasiabi Rad Nasrin Shadjou Mohammad-Reza Rashidi Mohammad Reza Afshar Mogaddam Abolghasem Jouyban |
| author_facet | Rokhsareh Ebrahimi Abolfazl Barzegari Reza Teimuri-Mofrad Houman Kholafazad Kordasht Mohammad Hasanzadeh Maryam Khoubnasabjafari Vahid Jouyban-Gharamaleki Abbas Afrasiabi Rad Nasrin Shadjou Mohammad-Reza Rashidi Mohammad Reza Afshar Mogaddam Abolghasem Jouyban |
| author_sort | Rokhsareh Ebrahimi |
| collection | DOAJ |
| description | For the first time, a novel aptamer was designed and utilized for the selective detection of rivaroxaban (RIV) using the integration of bioinformatics with biosensing technology. The selected aptamer with the sequence 5′-TAG GGA AGA GAA GGA CAT ATG ATG ACT CAC AAC TGG ACG AAC GTA CTT ATC CCC CCC AAT CAC TAG TGA ATT-3′ displayed a high binding affinity to RIV and had an efficient ability to discriminate RIV from similar molecular structures. A novel label-free electrochemical aptasensor was designed and fabricated through the conjugation of a thiolated aptamer with Au nanoparticles (Au-NPs). Then, the aptasensor was successfully applied for the quantitative determination of RIV in human plasma and exhaled breath condensate (EBC) samples with limits of detection (LODs) of 14.08 and 6.03 nM, respectively. These valuable results provide ample evidence of the green electrogeneration of AuNPs on the surface of electrodes and their interaction with loaded aptamers (based on Au-S binding) towards the sensitive and selective monitoring of RIV in human plasma and EBC samples. This bio-assay is an alternative approach for the clinical analysis of RIV and has improved specificity and affinity. As far as we know, this is the first time that an electrochemical aptasensor has been verified for the recognition of RIV and that allows for the easy, fast, and precise screening of RIV in biological samples. |
| first_indexed | 2024-03-09T20:36:11Z |
| format | Article |
| id | doaj.art-a0cf212101f24217a9b5c61fe977e3a4 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T20:36:11Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-a0cf212101f24217a9b5c61fe977e3a42023-11-23T23:10:25ZengMDPI AGBiosensors2079-63742022-09-01121077310.3390/bios12100773Selection of Specific Aptamer against Rivaroxaban and Utilization for Label-Free Electrochemical Aptasensing Using Gold Nanoparticles: First Announcement and Application for Clinical Sample AnalysisRokhsareh Ebrahimi0Abolfazl Barzegari1Reza Teimuri-Mofrad2Houman Kholafazad Kordasht3Mohammad Hasanzadeh4Maryam Khoubnasabjafari5Vahid Jouyban-Gharamaleki6Abbas Afrasiabi Rad7Nasrin Shadjou8Mohammad-Reza Rashidi9Mohammad Reza Afshar Mogaddam10Abolghasem Jouyban11Student Research Committee, Tabriz University of Medical Sciences, Tabriz 5165665811, IranResearch Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz 5165665811, IranDepartment of Organic and Biochemistry, Faculty of Chemistry, University of Tabriz, Tabriz 5166616471, IranBiotechnology Research Center, Tabriz University of Medical Sciences, Tabriz 5165665811, IranPharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz 5165665811, IranTuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz 5165665811, IranKimia Idea Pardaz Azarbayjan (KIPA) Science Based Company, Tabriz University of Medical Sciences, Tabriz 5165665811, IranCardiovascular Research Center, Tabriz University of Medical Sciences, Tabriz 5165665811, IranDepartment of Nanochemistry, Nanotechnology Research Center, Urmia University, Urmia 5756151818, IranMedicinal Chemistry Department, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz 5165665811, IranFood and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz 5165665811, IranPharmaceutical Analysis Research Center, Tabriz University of Medical Sciences, Tabriz 5165665811, IranFor the first time, a novel aptamer was designed and utilized for the selective detection of rivaroxaban (RIV) using the integration of bioinformatics with biosensing technology. The selected aptamer with the sequence 5′-TAG GGA AGA GAA GGA CAT ATG ATG ACT CAC AAC TGG ACG AAC GTA CTT ATC CCC CCC AAT CAC TAG TGA ATT-3′ displayed a high binding affinity to RIV and had an efficient ability to discriminate RIV from similar molecular structures. A novel label-free electrochemical aptasensor was designed and fabricated through the conjugation of a thiolated aptamer with Au nanoparticles (Au-NPs). Then, the aptasensor was successfully applied for the quantitative determination of RIV in human plasma and exhaled breath condensate (EBC) samples with limits of detection (LODs) of 14.08 and 6.03 nM, respectively. These valuable results provide ample evidence of the green electrogeneration of AuNPs on the surface of electrodes and their interaction with loaded aptamers (based on Au-S binding) towards the sensitive and selective monitoring of RIV in human plasma and EBC samples. This bio-assay is an alternative approach for the clinical analysis of RIV and has improved specificity and affinity. As far as we know, this is the first time that an electrochemical aptasensor has been verified for the recognition of RIV and that allows for the easy, fast, and precise screening of RIV in biological samples.https://www.mdpi.com/2079-6374/12/10/773rivaroxabanaptameraptasensorbioanalysis of drugsbiotechnologyadvanced nanomaterial |
| spellingShingle | Rokhsareh Ebrahimi Abolfazl Barzegari Reza Teimuri-Mofrad Houman Kholafazad Kordasht Mohammad Hasanzadeh Maryam Khoubnasabjafari Vahid Jouyban-Gharamaleki Abbas Afrasiabi Rad Nasrin Shadjou Mohammad-Reza Rashidi Mohammad Reza Afshar Mogaddam Abolghasem Jouyban Selection of Specific Aptamer against Rivaroxaban and Utilization for Label-Free Electrochemical Aptasensing Using Gold Nanoparticles: First Announcement and Application for Clinical Sample Analysis Biosensors rivaroxaban aptamer aptasensor bioanalysis of drugs biotechnology advanced nanomaterial |
| title | Selection of Specific Aptamer against Rivaroxaban and Utilization for Label-Free Electrochemical Aptasensing Using Gold Nanoparticles: First Announcement and Application for Clinical Sample Analysis |
| title_full | Selection of Specific Aptamer against Rivaroxaban and Utilization for Label-Free Electrochemical Aptasensing Using Gold Nanoparticles: First Announcement and Application for Clinical Sample Analysis |
| title_fullStr | Selection of Specific Aptamer against Rivaroxaban and Utilization for Label-Free Electrochemical Aptasensing Using Gold Nanoparticles: First Announcement and Application for Clinical Sample Analysis |
| title_full_unstemmed | Selection of Specific Aptamer against Rivaroxaban and Utilization for Label-Free Electrochemical Aptasensing Using Gold Nanoparticles: First Announcement and Application for Clinical Sample Analysis |
| title_short | Selection of Specific Aptamer against Rivaroxaban and Utilization for Label-Free Electrochemical Aptasensing Using Gold Nanoparticles: First Announcement and Application for Clinical Sample Analysis |
| title_sort | selection of specific aptamer against rivaroxaban and utilization for label free electrochemical aptasensing using gold nanoparticles first announcement and application for clinical sample analysis |
| topic | rivaroxaban aptamer aptasensor bioanalysis of drugs biotechnology advanced nanomaterial |
| url | https://www.mdpi.com/2079-6374/12/10/773 |
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