Estimating the genetic divergence and identification of three trichinella species by isoenzyme analysis
Isoenzyme-based approach was applied to compare Trichinella spiralis, T. britovi and T. pseudospiralis species. Among 13 enzyme systems examined, esterase (EST), malic enzyme (ME) and phosphoglucomutase (PGM) have been found as fully diagnostic, with no common allele in species studied. Adenosine de...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
2001-06-01
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| Series: | Parasite |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1051/parasite/200108s2030 |
| Summary: | Isoenzyme-based approach was applied to compare Trichinella spiralis, T. britovi and T. pseudospiralis species. Among 13 enzyme systems examined, esterase (EST), malic enzyme (ME) and phosphoglucomutase (PGM) have been found as fully diagnostic, with no common allele in species studied. Adenosine deaminase (ADA), adenylate kinase (AK), hexokinase (HK), peptidase leucyl-alanine (PEP-C) and fructose-bis-phosphatase (FBP) have been capable of distinguishing the two species from resulting profiles. In addition, ADA, AK and PGM displayed the enzyme expression in the lowest amounts of muscle larvae in systems tested ( 100 larvae/100 μl of extracts). Based on allozyme data, T. pseudospiralis has been found as the most distinct species within the group of taxa. Only a subtle genetic variability was recorded for T. pseudospiralisin which solely phosphoglucomutase exhibited variant patterns. In addition to the study of reference isolates, T. spiralis from lowland fox in Eastern Slovakia has been evidenced by use of genetic markers. This finding has proved that T. britovi is not the exclusive species parasitizing in the sylvatic ecosystem of the Slovak region. |
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| ISSN: | 1252-607X 1776-1042 |