Molecular Characterization of Giardia intestinalis and Cryptosporidium spp. Detected in Humans in Ağrı, Türkiye
Background: We aimed to determine the prevalence of Giardia intestinalis and Cryptosporidium spp. in patients who admitted hospital with diarrhea and to gain information about the transmission of these parasites in Ağrı, Türkiye. Methods: This study included 184 patients who applied to Ağrı-Diya...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2024-02-01
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| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3993 |
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| author | Selahattin Aydemir Fethi Barlık Abdurrahman Ekici Dilara Hande Barlık Sevil Alkan Esra Gürbüz Hasan Yilmaz |
| author_facet | Selahattin Aydemir Fethi Barlık Abdurrahman Ekici Dilara Hande Barlık Sevil Alkan Esra Gürbüz Hasan Yilmaz |
| author_sort | Selahattin Aydemir |
| collection | DOAJ |
| description |
Background: We aimed to determine the prevalence of Giardia intestinalis and Cryptosporidium spp. in patients who admitted hospital with diarrhea and to gain information about the transmission of these parasites in Ağrı, Türkiye.
Methods: This study included 184 patients who applied to Ağrı-Diyadin State Hospital, Türkiye in 2022. The immunochromatographic card test was used for detection of the G. intestinalis and Cryptosporidium spp. Nested PCR-RFLP analysis of the COWP gene and sequence analysis of the gp60 gene were used to genotype and subtype Cryptosporidium spp., whereas Nested PCR and sequence analyses of β-giardin gene were used genotype G. intestinalis.
Results: Of the 184 stool specimens examined, 12 (14.29%) and 7 (3.80%) were positive for G. intestinalis and Cryptosporidium spp., respectively. The Cryptosporidium species were identified as C. parvum belonging to the IId subtype family. The G. intestinalis were identified assemblages A.
Conclusion: Assemblage A, which is associated with diarrhea, is responsible for giardiasis and C. parvum IId subtype, often found in sheep, goats and cattle, is responsible for cryptosporidiosis in Ağrı, Türkiye.
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| first_indexed | 2024-04-25T01:00:45Z |
| format | Article |
| id | doaj.art-a0d70666e0ca4791b7795e5ce85f3ca2 |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-04-25T01:00:45Z |
| publishDate | 2024-02-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-a0d70666e0ca4791b7795e5ce85f3ca22024-03-11T05:30:07ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2024-02-01191Molecular Characterization of Giardia intestinalis and Cryptosporidium spp. Detected in Humans in Ağrı, TürkiyeSelahattin Aydemir0Fethi Barlık1Abdurrahman Ekici2Dilara Hande Barlık3Sevil Alkan4Esra Gürbüz5Hasan Yilmaz6Department of Parasitology, Faculty of Medicine, Van Yüzüncü Yıl University, Van, TurkeyDepartment of Parasitology, Faculty of Medicine, Van Yüzüncü Yıl University, Van, TurkeyDepartment of Parasitology, Faculty of Medicine, Van Yüzüncü Yıl University, Van, TurkeyDepartment of Molecular Biology and Genetics, Faculty of Science, Van Yüzüncü Yıl University, Van, TurkeyDepartment of Infectious Disease and Clinical Microbiology, Faculty of Medicine, Çanakkale Onsekiz Mart Uni-versity, Çanakkale, TurkeyInfectious Diseases and Clinical Microbiology, SBU Van Training and Research Hospital, Van, TurkeyDepartment of Parasitology, Faculty of Medicine, Van Yüzüncü Yıl University, Van, Turkey Background: We aimed to determine the prevalence of Giardia intestinalis and Cryptosporidium spp. in patients who admitted hospital with diarrhea and to gain information about the transmission of these parasites in Ağrı, Türkiye. Methods: This study included 184 patients who applied to Ağrı-Diyadin State Hospital, Türkiye in 2022. The immunochromatographic card test was used for detection of the G. intestinalis and Cryptosporidium spp. Nested PCR-RFLP analysis of the COWP gene and sequence analysis of the gp60 gene were used to genotype and subtype Cryptosporidium spp., whereas Nested PCR and sequence analyses of β-giardin gene were used genotype G. intestinalis. Results: Of the 184 stool specimens examined, 12 (14.29%) and 7 (3.80%) were positive for G. intestinalis and Cryptosporidium spp., respectively. The Cryptosporidium species were identified as C. parvum belonging to the IId subtype family. The G. intestinalis were identified assemblages A. Conclusion: Assemblage A, which is associated with diarrhea, is responsible for giardiasis and C. parvum IId subtype, often found in sheep, goats and cattle, is responsible for cryptosporidiosis in Ağrı, Türkiye. https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3993Cryptosporidium spp.EpidemiologyGenotypeGiardia intestinalisSubtypesTurkey |
| spellingShingle | Selahattin Aydemir Fethi Barlık Abdurrahman Ekici Dilara Hande Barlık Sevil Alkan Esra Gürbüz Hasan Yilmaz Molecular Characterization of Giardia intestinalis and Cryptosporidium spp. Detected in Humans in Ağrı, Türkiye Iranian Journal of Parasitology Cryptosporidium spp. Epidemiology Genotype Giardia intestinalis Subtypes Turkey |
| title | Molecular Characterization of Giardia intestinalis and Cryptosporidium spp. Detected in Humans in Ağrı, Türkiye |
| title_full | Molecular Characterization of Giardia intestinalis and Cryptosporidium spp. Detected in Humans in Ağrı, Türkiye |
| title_fullStr | Molecular Characterization of Giardia intestinalis and Cryptosporidium spp. Detected in Humans in Ağrı, Türkiye |
| title_full_unstemmed | Molecular Characterization of Giardia intestinalis and Cryptosporidium spp. Detected in Humans in Ağrı, Türkiye |
| title_short | Molecular Characterization of Giardia intestinalis and Cryptosporidium spp. Detected in Humans in Ağrı, Türkiye |
| title_sort | molecular characterization of giardia intestinalis and cryptosporidium spp detected in humans in agri turkiye |
| topic | Cryptosporidium spp. Epidemiology Genotype Giardia intestinalis Subtypes Turkey |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/3993 |
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