Platelet-activating factor acetylhydrolase and transacetylase activities in human aorta and mammary artery*
Platelet-activating factor (PAF), the potent phospholipid mediator of inflammation, is involved in atherosclerosis. Platelet-activating factor-acetylhydrolase (PAF-AH), the enzyme that inactivates PAF bioactivity, possesses both acetylhydrolase and transacetylase activities. In the present study, we...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2008-10-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520346472 |
| _version_ | 1831569420690915328 |
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| author | Demokritos C. Tsoukatos Isabelle Brochériou Vassilios Moussis Christina P. Panopoulou Elena D. Christofidou Stamatis Koussissis Socratis Sismanidis Ewa Ninio Stavros Siminelakis |
| author_facet | Demokritos C. Tsoukatos Isabelle Brochériou Vassilios Moussis Christina P. Panopoulou Elena D. Christofidou Stamatis Koussissis Socratis Sismanidis Ewa Ninio Stavros Siminelakis |
| author_sort | Demokritos C. Tsoukatos |
| collection | DOAJ |
| description | Platelet-activating factor (PAF), the potent phospholipid mediator of inflammation, is involved in atherosclerosis. Platelet-activating factor-acetylhydrolase (PAF-AH), the enzyme that inactivates PAF bioactivity, possesses both acetylhydrolase and transacetylase activities. In the present study, we measured acetylhydrolase and transacetylase activities in human atherogenic aorta and nonatherogenic mammary arteries. Immunohistochemistry analysis showed PAF-AH expression in the intima and the media of the aorta and in the media of mammary arteries. Acetylhydrolase and transacetylase activities were (mean ± SE, n = 38): acetylhydrolase of aorta, 2.8 ± 0.5 pmol/min/mg of tissue; transacetylase of aorta, 3.3 ± 0.7 pmol/min/mg of tissue; acetylhydrolase of mammary artery, 1.4 ± 0.3 pmol/min/mg of tissue (P < 0.004 as compared with acetylhydrolase of aorta); transacetylase of mammary artery, 0.8 ± 0.2 pmol/min/mg of tissue (P < 0.03 as compared with acetylhydrolase of mammary artery). Lyso-PAF accumulation and an increase in PAF bioactivity were observed in the aorta of some patients. Reverse-phase HPLC and electrospray ionization mass spectrometry analysis revealed that 1-O-hexadecyl-2 acetyl-sn glycero-3-phosphocholine accounted for 60% of the PAF bioactivity and 1-O-hexadecyl-2-butanoyl-sn-glycerol-3-phosphocholine for 40% of the PAF bioactivity. The nonatherogenic properties of mammary arteries may in part be due to low PAF formation regulated by PAF-AH activity. In atherogenic aortas, an imbalance between PAF-AH and transacetylase activity, as well as lyso-PAF accumulation, may lead to unregulated PAF formation and to progression of atherosclerosis. |
| first_indexed | 2024-12-17T12:33:18Z |
| format | Article |
| id | doaj.art-a0ddd16f82ee41389b8a6b58748a5971 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T12:33:18Z |
| publishDate | 2008-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-a0ddd16f82ee41389b8a6b58748a59712022-12-21T21:48:27ZengElsevierJournal of Lipid Research0022-22752008-10-01491022402249Platelet-activating factor acetylhydrolase and transacetylase activities in human aorta and mammary artery*Demokritos C. Tsoukatos0Isabelle Brochériou1Vassilios Moussis2Christina P. Panopoulou3Elena D. Christofidou4Stamatis Koussissis5Socratis Sismanidis6Ewa Ninio7Stavros Siminelakis8Department of Chemistry, University of Ioannina, Ioannina, GreeceService d'Anatomie-pathologie, Groupe Hospitalier Pitié-Salpêtrière, Paris, France; Institut national de la santé et de la recherche médicale, UMRS525, Faculté de Médecine, Université Pierre et Marie Curie, Paris, FranceDepartment of Chemistry, University of Ioannina, Ioannina, GreeceDepartment of Chemistry, University of Ioannina, Ioannina, GreeceDepartment of Chemistry, University of Ioannina, Ioannina, GreeceTechnological Educational Institution of Athens, Athens, GreeceClinic of Cardiac Surgery, University Hospital of Ioannina, Ioannina, GreeceInstitut national de la santé et de la recherche médicale, UMRS525, Faculté de Médecine, Université Pierre et Marie Curie, Paris, FranceClinic of Cardiac Surgery, University Hospital of Ioannina, Ioannina, GreecePlatelet-activating factor (PAF), the potent phospholipid mediator of inflammation, is involved in atherosclerosis. Platelet-activating factor-acetylhydrolase (PAF-AH), the enzyme that inactivates PAF bioactivity, possesses both acetylhydrolase and transacetylase activities. In the present study, we measured acetylhydrolase and transacetylase activities in human atherogenic aorta and nonatherogenic mammary arteries. Immunohistochemistry analysis showed PAF-AH expression in the intima and the media of the aorta and in the media of mammary arteries. Acetylhydrolase and transacetylase activities were (mean ± SE, n = 38): acetylhydrolase of aorta, 2.8 ± 0.5 pmol/min/mg of tissue; transacetylase of aorta, 3.3 ± 0.7 pmol/min/mg of tissue; acetylhydrolase of mammary artery, 1.4 ± 0.3 pmol/min/mg of tissue (P < 0.004 as compared with acetylhydrolase of aorta); transacetylase of mammary artery, 0.8 ± 0.2 pmol/min/mg of tissue (P < 0.03 as compared with acetylhydrolase of mammary artery). Lyso-PAF accumulation and an increase in PAF bioactivity were observed in the aorta of some patients. Reverse-phase HPLC and electrospray ionization mass spectrometry analysis revealed that 1-O-hexadecyl-2 acetyl-sn glycero-3-phosphocholine accounted for 60% of the PAF bioactivity and 1-O-hexadecyl-2-butanoyl-sn-glycerol-3-phosphocholine for 40% of the PAF bioactivity. The nonatherogenic properties of mammary arteries may in part be due to low PAF formation regulated by PAF-AH activity. In atherogenic aortas, an imbalance between PAF-AH and transacetylase activity, as well as lyso-PAF accumulation, may lead to unregulated PAF formation and to progression of atherosclerosis.http://www.sciencedirect.com/science/article/pii/S0022227520346472atherosclerosismacrophagessmooth muscle cellslipoproteinsatherogenesisoxidized LDL |
| spellingShingle | Demokritos C. Tsoukatos Isabelle Brochériou Vassilios Moussis Christina P. Panopoulou Elena D. Christofidou Stamatis Koussissis Socratis Sismanidis Ewa Ninio Stavros Siminelakis Platelet-activating factor acetylhydrolase and transacetylase activities in human aorta and mammary artery* Journal of Lipid Research atherosclerosis macrophages smooth muscle cells lipoproteins atherogenesis oxidized LDL |
| title | Platelet-activating factor acetylhydrolase and transacetylase activities in human aorta and mammary artery* |
| title_full | Platelet-activating factor acetylhydrolase and transacetylase activities in human aorta and mammary artery* |
| title_fullStr | Platelet-activating factor acetylhydrolase and transacetylase activities in human aorta and mammary artery* |
| title_full_unstemmed | Platelet-activating factor acetylhydrolase and transacetylase activities in human aorta and mammary artery* |
| title_short | Platelet-activating factor acetylhydrolase and transacetylase activities in human aorta and mammary artery* |
| title_sort | platelet activating factor acetylhydrolase and transacetylase activities in human aorta and mammary artery |
| topic | atherosclerosis macrophages smooth muscle cells lipoproteins atherogenesis oxidized LDL |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520346472 |
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