Genomic-Wide Identification and Characterization of the Uridine Diphosphate Glycosyltransferase Family in <i>Eucommia ulmoides</i> Oliver
<i>Eucommia ulmoides</i> Oliver is a woody plant with great economic and medicinal value. Its dried bark has a long history of use as a traditional medicinal material in East Asia, which led to many glycosides, such as aucubin, geniposide, hyperoside, astragalin, and pinoresinol diglucos...
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MDPI AG
2021-09-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/9/1934 |
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| author | Dan Ouyang Lan-Chun Wang Ting Tang Hong Feng |
| author_facet | Dan Ouyang Lan-Chun Wang Ting Tang Hong Feng |
| author_sort | Dan Ouyang |
| collection | DOAJ |
| description | <i>Eucommia ulmoides</i> Oliver is a woody plant with great economic and medicinal value. Its dried bark has a long history of use as a traditional medicinal material in East Asia, which led to many glycosides, such as aucubin, geniposide, hyperoside, astragalin, and pinoresinol diglucoside, being recognized as pharmacologically active ingredients. Uridine diphosphate glycosyltransferases (UGTs) catalyze a glycosyl-transferring reaction from the donor molecule uridine-5′-diphosphate-glucose (UDPG) to the substrate, which plays an important role in many biological processes, such as plant growth and development, secondary metabolism, and environmental adaptation. In order to explore the biosynthetic pathways of glycosides in <i>E. ulmoides</i>, 91 putative <i>Eu</i>UGT genes were identified throughout the complete genome of <i>E. ulmoides</i> through function annotation and an UDPGT domain search. Phylogenetic analysis categorized them into 14 groups. We also performed GO annotations on all the <i>Eu</i>UGTs to gain insights into their functions in <i>E. ulmoides</i>. In addition, transcriptomic analysis indicated that most <i>Eu</i>UGTs showed different expression patterns across diverse organs and various growing seasons. By protein–protein interaction predication, a biosynthetic routine of flavonoids and their glycosides was also proposed. Undoubtedly, these results will help in future research into the biosynthetic pathways of glycoside compounds in <i>E. ulmoides</i>. |
| first_indexed | 2024-03-10T07:17:33Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T07:17:33Z |
| publishDate | 2021-09-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-a0de187bf1aa4cf988438a35d1df9d7f2023-11-22T14:53:59ZengMDPI AGPlants2223-77472021-09-01109193410.3390/plants10091934Genomic-Wide Identification and Characterization of the Uridine Diphosphate Glycosyltransferase Family in <i>Eucommia ulmoides</i> OliverDan Ouyang0Lan-Chun Wang1Ting Tang2Hong Feng3Key Laboratory of Molecular Biology and Biotechnology of Sichuan Province, Department of Biotechnology, College of Life Sciences, Sichuan University, Chengdu 610064, ChinaKey Laboratory of Molecular Biology and Biotechnology of Sichuan Province, Department of Biotechnology, College of Life Sciences, Sichuan University, Chengdu 610064, ChinaKey Laboratory of Molecular Biology and Biotechnology of Sichuan Province, Department of Biotechnology, College of Life Sciences, Sichuan University, Chengdu 610064, ChinaKey Laboratory of Molecular Biology and Biotechnology of Sichuan Province, Department of Biotechnology, College of Life Sciences, Sichuan University, Chengdu 610064, China<i>Eucommia ulmoides</i> Oliver is a woody plant with great economic and medicinal value. Its dried bark has a long history of use as a traditional medicinal material in East Asia, which led to many glycosides, such as aucubin, geniposide, hyperoside, astragalin, and pinoresinol diglucoside, being recognized as pharmacologically active ingredients. Uridine diphosphate glycosyltransferases (UGTs) catalyze a glycosyl-transferring reaction from the donor molecule uridine-5′-diphosphate-glucose (UDPG) to the substrate, which plays an important role in many biological processes, such as plant growth and development, secondary metabolism, and environmental adaptation. In order to explore the biosynthetic pathways of glycosides in <i>E. ulmoides</i>, 91 putative <i>Eu</i>UGT genes were identified throughout the complete genome of <i>E. ulmoides</i> through function annotation and an UDPGT domain search. Phylogenetic analysis categorized them into 14 groups. We also performed GO annotations on all the <i>Eu</i>UGTs to gain insights into their functions in <i>E. ulmoides</i>. In addition, transcriptomic analysis indicated that most <i>Eu</i>UGTs showed different expression patterns across diverse organs and various growing seasons. By protein–protein interaction predication, a biosynthetic routine of flavonoids and their glycosides was also proposed. Undoubtedly, these results will help in future research into the biosynthetic pathways of glycoside compounds in <i>E. ulmoides</i>.https://www.mdpi.com/2223-7747/10/9/1934<i>Eucommia</i> <i>ulmoides</i>UDP-glycosyltransferasetranscriptomeexpressionlignanflavonoid |
| spellingShingle | Dan Ouyang Lan-Chun Wang Ting Tang Hong Feng Genomic-Wide Identification and Characterization of the Uridine Diphosphate Glycosyltransferase Family in <i>Eucommia ulmoides</i> Oliver Plants <i>Eucommia</i> <i>ulmoides</i> UDP-glycosyltransferase transcriptome expression lignan flavonoid |
| title | Genomic-Wide Identification and Characterization of the Uridine Diphosphate Glycosyltransferase Family in <i>Eucommia ulmoides</i> Oliver |
| title_full | Genomic-Wide Identification and Characterization of the Uridine Diphosphate Glycosyltransferase Family in <i>Eucommia ulmoides</i> Oliver |
| title_fullStr | Genomic-Wide Identification and Characterization of the Uridine Diphosphate Glycosyltransferase Family in <i>Eucommia ulmoides</i> Oliver |
| title_full_unstemmed | Genomic-Wide Identification and Characterization of the Uridine Diphosphate Glycosyltransferase Family in <i>Eucommia ulmoides</i> Oliver |
| title_short | Genomic-Wide Identification and Characterization of the Uridine Diphosphate Glycosyltransferase Family in <i>Eucommia ulmoides</i> Oliver |
| title_sort | genomic wide identification and characterization of the uridine diphosphate glycosyltransferase family in i eucommia ulmoides i oliver |
| topic | <i>Eucommia</i> <i>ulmoides</i> UDP-glycosyltransferase transcriptome expression lignan flavonoid |
| url | https://www.mdpi.com/2223-7747/10/9/1934 |
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