Identification of heterosomes in spermatoza of rams with 54,XX/54,XY chimerism
The aim of the study was to identify heterosomes in the semen of three Romanov rams - carriers of leukocyte chimerism (FISH technique) and to determine the proportions between spermatozoa with X and Y chromosomes. The choice of bovine probes for hybridization with ram heterosomes was dictated by gen...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2008-05-01
|
| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-200805-0004_identification-of-heterosomes-in-spermatoza-of-rams-with-54-xx-54-xy-chimerism.php |
| _version_ | 1797897678494892032 |
|---|---|
| author | A. Kozubska-Sobocinska B. Rejduch |
| author_facet | A. Kozubska-Sobocinska B. Rejduch |
| author_sort | A. Kozubska-Sobocinska |
| collection | DOAJ |
| description | The aim of the study was to identify heterosomes in the semen of three Romanov rams - carriers of leukocyte chimerism (FISH technique) and to determine the proportions between spermatozoa with X and Y chromosomes. The choice of bovine probes for hybridization with ram heterosomes was dictated by genetic conservatism of bovine and ovine heterosomes. The ratio between spermatozoa with a yellow fluorescent signal containing the X chromosome in the haploid set and spermatozoa with a red-purple signal indicating the presence of the Y chromosome, taking into account spermatozoa with no signal, was 52%:43%:5% in ram No. PL100006077676; 47%:44%:9% in ram No. PL100006078031; and 48%:46%:6% in ram No. PL100006078895. The results obtained lead us to conclude that the 54,XX/54,XY chimerism has no effect on sex ratio in offspring. |
| first_indexed | 2024-04-10T08:02:07Z |
| format | Article |
| id | doaj.art-a0dfa609ef29467da89c57dd738ac443 |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T08:02:07Z |
| publishDate | 2008-05-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-a0dfa609ef29467da89c57dd738ac4432023-02-23T03:49:34ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922008-05-0153525025410.17221/1855-VETMEDvet-200805-0004Identification of heterosomes in spermatoza of rams with 54,XX/54,XY chimerismA. Kozubska-Sobocinska0B. Rejduch1National Research Institute of Animal Production, Balice n. Krakow, PolandNational Research Institute of Animal Production, Balice n. Krakow, PolandThe aim of the study was to identify heterosomes in the semen of three Romanov rams - carriers of leukocyte chimerism (FISH technique) and to determine the proportions between spermatozoa with X and Y chromosomes. The choice of bovine probes for hybridization with ram heterosomes was dictated by genetic conservatism of bovine and ovine heterosomes. The ratio between spermatozoa with a yellow fluorescent signal containing the X chromosome in the haploid set and spermatozoa with a red-purple signal indicating the presence of the Y chromosome, taking into account spermatozoa with no signal, was 52%:43%:5% in ram No. PL100006077676; 47%:44%:9% in ram No. PL100006078031; and 48%:46%:6% in ram No. PL100006078895. The results obtained lead us to conclude that the 54,XX/54,XY chimerism has no effect on sex ratio in offspring.https://vetmed.agriculturejournals.cz/artkey/vet-200805-0004_identification-of-heterosomes-in-spermatoza-of-rams-with-54-xx-54-xy-chimerism.phpsheepleukocyte chimerismfishspermatozoaheterosomes |
| spellingShingle | A. Kozubska-Sobocinska B. Rejduch Identification of heterosomes in spermatoza of rams with 54,XX/54,XY chimerism Veterinární Medicína sheep leukocyte chimerism fish spermatozoa heterosomes |
| title | Identification of heterosomes in spermatoza of rams with 54,XX/54,XY chimerism |
| title_full | Identification of heterosomes in spermatoza of rams with 54,XX/54,XY chimerism |
| title_fullStr | Identification of heterosomes in spermatoza of rams with 54,XX/54,XY chimerism |
| title_full_unstemmed | Identification of heterosomes in spermatoza of rams with 54,XX/54,XY chimerism |
| title_short | Identification of heterosomes in spermatoza of rams with 54,XX/54,XY chimerism |
| title_sort | identification of heterosomes in spermatoza of rams with 54 xx 54 xy chimerism |
| topic | sheep leukocyte chimerism fish spermatozoa heterosomes |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-200805-0004_identification-of-heterosomes-in-spermatoza-of-rams-with-54-xx-54-xy-chimerism.php |
| work_keys_str_mv | AT akozubskasobocinska identificationofheterosomesinspermatozaoframswith54xx54xychimerism AT brejduch identificationofheterosomesinspermatozaoframswith54xx54xychimerism |