Short-Term Alternate Feeding between Terrestrially Sourced Oil- and Fish Oil-Based Diets Modulates the Intestinal Microecology of Juvenile Turbot
A nine-week feeding trial was conducted to investigate changes in the intestinal microbiota of turbot in response to alternate feeding between terrestrially sourced oil (TSO)- and fish oil (FO)-based diets. The following three feeding strategies were designed: (1) continuous feeding with the FO-base...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
|
| Series: | Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-7737/12/5/650 |
| _version_ | 1797601045046624256 |
|---|---|
| author | Xiuhua Ma Yaoyao Kong Houguo Xu Qingzhu Bi Mengqing Liang Kangsen Mai Yanjiao Zhang |
| author_facet | Xiuhua Ma Yaoyao Kong Houguo Xu Qingzhu Bi Mengqing Liang Kangsen Mai Yanjiao Zhang |
| author_sort | Xiuhua Ma |
| collection | DOAJ |
| description | A nine-week feeding trial was conducted to investigate changes in the intestinal microbiota of turbot in response to alternate feeding between terrestrially sourced oil (TSO)- and fish oil (FO)-based diets. The following three feeding strategies were designed: (1) continuous feeding with the FO-based diet (FO group); (2) weekly alternate feeding between soybean oil (SO)- and FO-based diets (SO/FO group); and (3) weekly alternate feeding between beef tallow (BT)- and FO-based diets (BT/FO group). An intestinal bacterial community analysis showed that alternate feeding reshaped the intestinal microbial composition. Higher species richness and diversity of the intestinal microbiota were observed in the alternate-feeding groups. A PCoA analysis showed that the samples clustered separately according to the feeding strategy, and among the three groups, the SO/FO group clustered relatively closer to the BT/FO group. The alternate feeding significantly decreased the abundance of <i>Mycoplasma</i> and selectively enriched specific microorganisms, including short-chain fatty acid (SCFA)-producing bacteria, digestive bacteria (<i>Corynebacterium</i> and <i>Sphingomonas</i>), and several potential pathogens (<i>Desulfovibrio</i> and <i>Mycobacterium</i>). Alternate feeding may maintain the intestinal microbiota balance by improving the connectivity of the ecological network and increasing the competitive interactions within the ecological network. The alternate feeding significantly upregulated the KEGG pathways of fatty acid and lipid metabolism, glycan biosynthesis, and amino acid metabolism in the intestinal microbiota. Meanwhile, the upregulation of the KEGG pathway of lipopolysaccharide biosynthesis indicates a potential risk for intestinal health. In conclusion, short-term alternate feeding between dietary lipid sources reshapes the intestinal microecology of the juvenile turbot, possibly resulting in both positive and negative effects. |
| first_indexed | 2024-03-11T03:55:55Z |
| format | Article |
| id | doaj.art-a0e0050bc54a42908d7f9bf5235e62f6 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-11T03:55:55Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-a0e0050bc54a42908d7f9bf5235e62f62023-11-18T00:32:27ZengMDPI AGBiology2079-77372023-04-0112565010.3390/biology12050650Short-Term Alternate Feeding between Terrestrially Sourced Oil- and Fish Oil-Based Diets Modulates the Intestinal Microecology of Juvenile TurbotXiuhua Ma0Yaoyao Kong1Houguo Xu2Qingzhu Bi3Mengqing Liang4Kangsen Mai5Yanjiao Zhang6Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs) & Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao 266003, ChinaKey Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs) & Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao 266003, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaYellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, ChinaKey Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs) & Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao 266003, ChinaKey Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture and Rural Affairs) & Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao 266003, ChinaA nine-week feeding trial was conducted to investigate changes in the intestinal microbiota of turbot in response to alternate feeding between terrestrially sourced oil (TSO)- and fish oil (FO)-based diets. The following three feeding strategies were designed: (1) continuous feeding with the FO-based diet (FO group); (2) weekly alternate feeding between soybean oil (SO)- and FO-based diets (SO/FO group); and (3) weekly alternate feeding between beef tallow (BT)- and FO-based diets (BT/FO group). An intestinal bacterial community analysis showed that alternate feeding reshaped the intestinal microbial composition. Higher species richness and diversity of the intestinal microbiota were observed in the alternate-feeding groups. A PCoA analysis showed that the samples clustered separately according to the feeding strategy, and among the three groups, the SO/FO group clustered relatively closer to the BT/FO group. The alternate feeding significantly decreased the abundance of <i>Mycoplasma</i> and selectively enriched specific microorganisms, including short-chain fatty acid (SCFA)-producing bacteria, digestive bacteria (<i>Corynebacterium</i> and <i>Sphingomonas</i>), and several potential pathogens (<i>Desulfovibrio</i> and <i>Mycobacterium</i>). Alternate feeding may maintain the intestinal microbiota balance by improving the connectivity of the ecological network and increasing the competitive interactions within the ecological network. The alternate feeding significantly upregulated the KEGG pathways of fatty acid and lipid metabolism, glycan biosynthesis, and amino acid metabolism in the intestinal microbiota. Meanwhile, the upregulation of the KEGG pathway of lipopolysaccharide biosynthesis indicates a potential risk for intestinal health. In conclusion, short-term alternate feeding between dietary lipid sources reshapes the intestinal microecology of the juvenile turbot, possibly resulting in both positive and negative effects.https://www.mdpi.com/2079-7737/12/5/650dietary lipid sourcesoybean oilbeef tallowgastro-intestinal tractintestinal health<i>Scophthalmus maximus</i> |
| spellingShingle | Xiuhua Ma Yaoyao Kong Houguo Xu Qingzhu Bi Mengqing Liang Kangsen Mai Yanjiao Zhang Short-Term Alternate Feeding between Terrestrially Sourced Oil- and Fish Oil-Based Diets Modulates the Intestinal Microecology of Juvenile Turbot Biology dietary lipid source soybean oil beef tallow gastro-intestinal tract intestinal health <i>Scophthalmus maximus</i> |
| title | Short-Term Alternate Feeding between Terrestrially Sourced Oil- and Fish Oil-Based Diets Modulates the Intestinal Microecology of Juvenile Turbot |
| title_full | Short-Term Alternate Feeding between Terrestrially Sourced Oil- and Fish Oil-Based Diets Modulates the Intestinal Microecology of Juvenile Turbot |
| title_fullStr | Short-Term Alternate Feeding between Terrestrially Sourced Oil- and Fish Oil-Based Diets Modulates the Intestinal Microecology of Juvenile Turbot |
| title_full_unstemmed | Short-Term Alternate Feeding between Terrestrially Sourced Oil- and Fish Oil-Based Diets Modulates the Intestinal Microecology of Juvenile Turbot |
| title_short | Short-Term Alternate Feeding between Terrestrially Sourced Oil- and Fish Oil-Based Diets Modulates the Intestinal Microecology of Juvenile Turbot |
| title_sort | short term alternate feeding between terrestrially sourced oil and fish oil based diets modulates the intestinal microecology of juvenile turbot |
| topic | dietary lipid source soybean oil beef tallow gastro-intestinal tract intestinal health <i>Scophthalmus maximus</i> |
| url | https://www.mdpi.com/2079-7737/12/5/650 |
| work_keys_str_mv | AT xiuhuama shorttermalternatefeedingbetweenterrestriallysourcedoilandfishoilbaseddietsmodulatestheintestinalmicroecologyofjuvenileturbot AT yaoyaokong shorttermalternatefeedingbetweenterrestriallysourcedoilandfishoilbaseddietsmodulatestheintestinalmicroecologyofjuvenileturbot AT houguoxu shorttermalternatefeedingbetweenterrestriallysourcedoilandfishoilbaseddietsmodulatestheintestinalmicroecologyofjuvenileturbot AT qingzhubi shorttermalternatefeedingbetweenterrestriallysourcedoilandfishoilbaseddietsmodulatestheintestinalmicroecologyofjuvenileturbot AT mengqingliang shorttermalternatefeedingbetweenterrestriallysourcedoilandfishoilbaseddietsmodulatestheintestinalmicroecologyofjuvenileturbot AT kangsenmai shorttermalternatefeedingbetweenterrestriallysourcedoilandfishoilbaseddietsmodulatestheintestinalmicroecologyofjuvenileturbot AT yanjiaozhang shorttermalternatefeedingbetweenterrestriallysourcedoilandfishoilbaseddietsmodulatestheintestinalmicroecologyofjuvenileturbot |