Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice
In this study, the polysaccharides extracted from Dendrobium officinale leaf (DOLP) was used in immune deficiency mice to evaluate the bioactivity. Thymus and spleen indices were calculated while the alleviation of the colon and liver histopathological progression was evaluated by H&E staining....
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157522000335 |
| _version_ | 1811311423703220224 |
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| author | Hualing Xie Jingyu Fang Mohamed A. Farag Zhenhao Li Peilong Sun Ping Shao |
| author_facet | Hualing Xie Jingyu Fang Mohamed A. Farag Zhenhao Li Peilong Sun Ping Shao |
| author_sort | Hualing Xie |
| collection | DOAJ |
| description | In this study, the polysaccharides extracted from Dendrobium officinale leaf (DOLP) was used in immune deficiency mice to evaluate the bioactivity. Thymus and spleen indices were calculated while the alleviation of the colon and liver histopathological progression was evaluated by H&E staining. The data indicated that DOLP improved immunity status by restoring the gut barrier and atrophy of immune organs. Cytokines levels as marker of inflammation were determined using ELISA in serum and colon. Which proved that DOLP inhibited the expression of pro-inflammatory cytokines (TNF-α, TGF- β1, IL-6, IL-1β) and promoted the expression of anti-inflammatory cytokines (IL-10). Short chain fatty acids (SCFAs) levels and microbial composition in feces were determined using GS and high-throughput sequencing. DOLP improved gut microbiota by increasing the relative abundance of total bacteria and probiotics such as Bacteroides, Lactobacillus and Lachnospiraceae. Therefore, DOLP has potential effect for the treatment of chronic immune diseases. |
| first_indexed | 2024-04-13T10:17:39Z |
| format | Article |
| id | doaj.art-a0e1e00830d4436c934060c231e6780a |
| institution | Directory Open Access Journal |
| issn | 2590-1575 |
| language | English |
| last_indexed | 2024-04-13T10:17:39Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj.art-a0e1e00830d4436c934060c231e6780a2022-12-22T02:50:39ZengElsevierFood Chemistry: X2590-15752022-03-0113100235Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated miceHualing Xie0Jingyu Fang1Mohamed A. Farag2Zhenhao Li3Peilong Sun4Ping Shao5College of Food Science and Technology, Zhejiang University of Technology, Zhejiang, Hangzhou 310014, PR ChinaCollege of Food Science and Technology, Zhejiang University of Technology, Zhejiang, Hangzhou 310014, PR ChinaPharmacognosy Department, College of Pharmacy, Cairo University, Kasr El Aini St. P.B, Cairo, Egypt; Department of Chemistry, School of Science & Engineering, The American University in Cairo, New Cairo 11835, EgyptZhejiang ShouXianGu Botanical Drug Institute Co. Ltd, Zhejiang, Hangzhou 321200, ChinaCollege of Food Science and Technology, Zhejiang University of Technology, Zhejiang, Hangzhou 310014, PR China; Corresponding authors.College of Food Science and Technology, Zhejiang University of Technology, Zhejiang, Hangzhou 310014, PR China; Corresponding authors.In this study, the polysaccharides extracted from Dendrobium officinale leaf (DOLP) was used in immune deficiency mice to evaluate the bioactivity. Thymus and spleen indices were calculated while the alleviation of the colon and liver histopathological progression was evaluated by H&E staining. The data indicated that DOLP improved immunity status by restoring the gut barrier and atrophy of immune organs. Cytokines levels as marker of inflammation were determined using ELISA in serum and colon. Which proved that DOLP inhibited the expression of pro-inflammatory cytokines (TNF-α, TGF- β1, IL-6, IL-1β) and promoted the expression of anti-inflammatory cytokines (IL-10). Short chain fatty acids (SCFAs) levels and microbial composition in feces were determined using GS and high-throughput sequencing. DOLP improved gut microbiota by increasing the relative abundance of total bacteria and probiotics such as Bacteroides, Lactobacillus and Lachnospiraceae. Therefore, DOLP has potential effect for the treatment of chronic immune diseases.http://www.sciencedirect.com/science/article/pii/S2590157522000335CyclophosphamideDendrobium officinalePolysaccharide fermentationImmune systemGut microbiotaLiver protection |
| spellingShingle | Hualing Xie Jingyu Fang Mohamed A. Farag Zhenhao Li Peilong Sun Ping Shao Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice Food Chemistry: X Cyclophosphamide Dendrobium officinale Polysaccharide fermentation Immune system Gut microbiota Liver protection |
| title | Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice |
| title_full | Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice |
| title_fullStr | Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice |
| title_full_unstemmed | Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice |
| title_short | Dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice |
| title_sort | dendrobium officinale leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide treated mice |
| topic | Cyclophosphamide Dendrobium officinale Polysaccharide fermentation Immune system Gut microbiota Liver protection |
| url | http://www.sciencedirect.com/science/article/pii/S2590157522000335 |
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