Freeze-fracture replica immunolabelling reveals urothelial plaques in cultured urothelial cells.
The primary function of the urothelium is to provide the tightest and most impermeable barrier in the body, i.e. the blood-urine barrier. Urothelial plaques are formed and inserted into the apical plasma membrane during advanced stages of urothelial cell differentiation. Currently, it is supposed th...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3387185?pdf=render |
| _version_ | 1818036755945750528 |
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| author | Mateja Erdani Kreft Horst Robenek |
| author_facet | Mateja Erdani Kreft Horst Robenek |
| author_sort | Mateja Erdani Kreft |
| collection | DOAJ |
| description | The primary function of the urothelium is to provide the tightest and most impermeable barrier in the body, i.e. the blood-urine barrier. Urothelial plaques are formed and inserted into the apical plasma membrane during advanced stages of urothelial cell differentiation. Currently, it is supposed that differentiation with the final formation of urothelial plaques is hindered in cultured urothelial cells. With the aid of the high-resolution imaging technique of freeze-fracture replica immunolabelling, we here provide evidence that urothelial cells in vitro form uroplakin-positive urothelial plaques, localized in fusiform-shaped vesicles and apical plasma membranes. With the establishment of such an in vitro model of urothelial cells with fully developed urothelial plaques and functional properties equivalent to normal bladder urothelium, new perspectives have emerged which challenge prevailing concepts of apical plasma membrane biogenesis and blood-urine barrier development. This may hopefully provide a timely impulse for many ongoing studies and open up new questions for future research. |
| first_indexed | 2024-12-10T07:16:00Z |
| format | Article |
| id | doaj.art-a0e22d9c10ec4cd28fc68b2951e1e927 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T07:16:00Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a0e22d9c10ec4cd28fc68b2951e1e9272022-12-22T01:57:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0176e3850910.1371/journal.pone.0038509Freeze-fracture replica immunolabelling reveals urothelial plaques in cultured urothelial cells.Mateja Erdani KreftHorst RobenekThe primary function of the urothelium is to provide the tightest and most impermeable barrier in the body, i.e. the blood-urine barrier. Urothelial plaques are formed and inserted into the apical plasma membrane during advanced stages of urothelial cell differentiation. Currently, it is supposed that differentiation with the final formation of urothelial plaques is hindered in cultured urothelial cells. With the aid of the high-resolution imaging technique of freeze-fracture replica immunolabelling, we here provide evidence that urothelial cells in vitro form uroplakin-positive urothelial plaques, localized in fusiform-shaped vesicles and apical plasma membranes. With the establishment of such an in vitro model of urothelial cells with fully developed urothelial plaques and functional properties equivalent to normal bladder urothelium, new perspectives have emerged which challenge prevailing concepts of apical plasma membrane biogenesis and blood-urine barrier development. This may hopefully provide a timely impulse for many ongoing studies and open up new questions for future research.http://europepmc.org/articles/PMC3387185?pdf=render |
| spellingShingle | Mateja Erdani Kreft Horst Robenek Freeze-fracture replica immunolabelling reveals urothelial plaques in cultured urothelial cells. PLoS ONE |
| title | Freeze-fracture replica immunolabelling reveals urothelial plaques in cultured urothelial cells. |
| title_full | Freeze-fracture replica immunolabelling reveals urothelial plaques in cultured urothelial cells. |
| title_fullStr | Freeze-fracture replica immunolabelling reveals urothelial plaques in cultured urothelial cells. |
| title_full_unstemmed | Freeze-fracture replica immunolabelling reveals urothelial plaques in cultured urothelial cells. |
| title_short | Freeze-fracture replica immunolabelling reveals urothelial plaques in cultured urothelial cells. |
| title_sort | freeze fracture replica immunolabelling reveals urothelial plaques in cultured urothelial cells |
| url | http://europepmc.org/articles/PMC3387185?pdf=render |
| work_keys_str_mv | AT matejaerdanikreft freezefracturereplicaimmunolabellingrevealsurothelialplaquesinculturedurothelialcells AT horstrobenek freezefracturereplicaimmunolabellingrevealsurothelialplaquesinculturedurothelialcells |