Genomic analysis revealed a novel genotype of methicillin-susceptible Staphylococcus aureus isolated from a fatal sepsis case in dengue patient
Abstract Staphylococcus aureus (S. aureus) is an opportunistic pathogen capable of causing serious health implications in susceptible individuals once it invades the host’s protective barriers. Methicillin-susceptible S. aureus (MSSA) often receives lesser attention although it has been frequently a...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-03-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-83661-8 |
| _version_ | 1818735296077889536 |
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| author | Soo Tein Ngoi Wen Kiong Niek Yee Wan Lee Sazaly AbuBakar Cindy Shuan Ju Teh |
| author_facet | Soo Tein Ngoi Wen Kiong Niek Yee Wan Lee Sazaly AbuBakar Cindy Shuan Ju Teh |
| author_sort | Soo Tein Ngoi |
| collection | DOAJ |
| description | Abstract Staphylococcus aureus (S. aureus) is an opportunistic pathogen capable of causing serious health implications in susceptible individuals once it invades the host’s protective barriers. Methicillin-susceptible S. aureus (MSSA) often receives lesser attention although it has been frequently associated with serious infections in human. We aim to investigate the genomic features of a highly virulent yet pan susceptible MSSA strain (coded as HS-MSSA) which caused concurrent bacteraemia in a dengue patient, ultimately resulted in sepsis death of the patient. Whole genome sequence analysis was performed. The draft genome of HS-MSSA is approximately 2.78 Mb (GC content = 32.7%) comprising of 2637 predicted coding sequences. In silico genotyping of the HS-MSSA strain revealed a novel combined genotype (t091/ST2990). The HS-MSSA carries a SaPIn1-like pathogenicity island that harbours the staphylococcal enterotoxin and enterotoxin-like genes (sec3 and selL). The strain-specific β-lactamase (blaZ)-bearing plasmid region was identified in HS-MSSA. Core genome phylogeny showed that the HS-MSSA strain shared a common ancestry with the European MRSA clone. We report herein the genomic features of an MSSA lineage with novel genotype previously not reported elsewhere. |
| first_indexed | 2024-12-18T00:19:00Z |
| format | Article |
| id | doaj.art-a0e44da886434c0592a09bea689ce53f |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T00:19:00Z |
| publishDate | 2021-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a0e44da886434c0592a09bea689ce53f2022-12-21T21:27:24ZengNature PortfolioScientific Reports2045-23222021-03-0111111210.1038/s41598-021-83661-8Genomic analysis revealed a novel genotype of methicillin-susceptible Staphylococcus aureus isolated from a fatal sepsis case in dengue patientSoo Tein Ngoi0Wen Kiong Niek1Yee Wan Lee2Sazaly AbuBakar3Cindy Shuan Ju Teh4Department of Medical Microbiology, Faculty of Medicine, University of MalayaDepartment of Medical Microbiology, Faculty of Medicine, University of MalayaDepartment of Medicine, Faculty of Medicine, University of MalayaDepartment of Medical Microbiology, Faculty of Medicine, University of MalayaDepartment of Medical Microbiology, Faculty of Medicine, University of MalayaAbstract Staphylococcus aureus (S. aureus) is an opportunistic pathogen capable of causing serious health implications in susceptible individuals once it invades the host’s protective barriers. Methicillin-susceptible S. aureus (MSSA) often receives lesser attention although it has been frequently associated with serious infections in human. We aim to investigate the genomic features of a highly virulent yet pan susceptible MSSA strain (coded as HS-MSSA) which caused concurrent bacteraemia in a dengue patient, ultimately resulted in sepsis death of the patient. Whole genome sequence analysis was performed. The draft genome of HS-MSSA is approximately 2.78 Mb (GC content = 32.7%) comprising of 2637 predicted coding sequences. In silico genotyping of the HS-MSSA strain revealed a novel combined genotype (t091/ST2990). The HS-MSSA carries a SaPIn1-like pathogenicity island that harbours the staphylococcal enterotoxin and enterotoxin-like genes (sec3 and selL). The strain-specific β-lactamase (blaZ)-bearing plasmid region was identified in HS-MSSA. Core genome phylogeny showed that the HS-MSSA strain shared a common ancestry with the European MRSA clone. We report herein the genomic features of an MSSA lineage with novel genotype previously not reported elsewhere.https://doi.org/10.1038/s41598-021-83661-8 |
| spellingShingle | Soo Tein Ngoi Wen Kiong Niek Yee Wan Lee Sazaly AbuBakar Cindy Shuan Ju Teh Genomic analysis revealed a novel genotype of methicillin-susceptible Staphylococcus aureus isolated from a fatal sepsis case in dengue patient Scientific Reports |
| title | Genomic analysis revealed a novel genotype of methicillin-susceptible Staphylococcus aureus isolated from a fatal sepsis case in dengue patient |
| title_full | Genomic analysis revealed a novel genotype of methicillin-susceptible Staphylococcus aureus isolated from a fatal sepsis case in dengue patient |
| title_fullStr | Genomic analysis revealed a novel genotype of methicillin-susceptible Staphylococcus aureus isolated from a fatal sepsis case in dengue patient |
| title_full_unstemmed | Genomic analysis revealed a novel genotype of methicillin-susceptible Staphylococcus aureus isolated from a fatal sepsis case in dengue patient |
| title_short | Genomic analysis revealed a novel genotype of methicillin-susceptible Staphylococcus aureus isolated from a fatal sepsis case in dengue patient |
| title_sort | genomic analysis revealed a novel genotype of methicillin susceptible staphylococcus aureus isolated from a fatal sepsis case in dengue patient |
| url | https://doi.org/10.1038/s41598-021-83661-8 |
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