Mass Spectrometry for Analysis of Cancer Biomarker from Human Plasma
Mass spectrometry (MS) can identify new proteins and assay the amount of the biomarker proteins that need to be validated as a viable cancer candidate. Low abundant peptides from plasma glycoprotein were identified by ultra high resolution 15T MALDI-FTICR MS with stable isotope standards and capture...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
SpringerOpen
2011-12-01
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| Series: | Journal of Analytical Science and Technology |
| Subjects: | |
| Online Access: | http://www.jastmag.org/journal/view.php?Type=C&number=72 |
| _version_ | 1811201490290737152 |
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| author | Yeong Hee Ahn Kwang Hoe Kim Park Min Shin Eun Sun Ji Jong Shin Yoo |
| author_facet | Yeong Hee Ahn Kwang Hoe Kim Park Min Shin Eun Sun Ji Jong Shin Yoo |
| author_sort | Yeong Hee Ahn |
| collection | DOAJ |
| description | Mass spectrometry (MS) can identify new proteins and assay the amount of the biomarker proteins that need to be validated as a viable cancer candidate. Low abundant peptides from plasma glycoprotein were identified by ultra high resolution 15T MALDI-FTICR MS with stable isotope standards and capture by anti-peptide antibodies. For the quantitation of the low abundant biomarkers, multiple reaction monitoring (MRM) method with ESI was applied for human plasma. |
| first_indexed | 2024-04-12T02:22:31Z |
| format | Article |
| id | doaj.art-a0e9160c5a8744ba9cbcef72e9e0ffed |
| institution | Directory Open Access Journal |
| issn | 2093-3134 2093-3371 |
| language | English |
| last_indexed | 2024-04-12T02:22:31Z |
| publishDate | 2011-12-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Analytical Science and Technology |
| spelling | doaj.art-a0e9160c5a8744ba9cbcef72e9e0ffed2022-12-22T03:52:06ZengSpringerOpenJournal of Analytical Science and Technology2093-31342093-33712011-12-012Supplement AA135A139Mass Spectrometry for Analysis of Cancer Biomarker from Human PlasmaYeong Hee AhnKwang Hoe KimPark Min ShinEun Sun JiJong Shin YooMass spectrometry (MS) can identify new proteins and assay the amount of the biomarker proteins that need to be validated as a viable cancer candidate. Low abundant peptides from plasma glycoprotein were identified by ultra high resolution 15T MALDI-FTICR MS with stable isotope standards and capture by anti-peptide antibodies. For the quantitation of the low abundant biomarkers, multiple reaction monitoring (MRM) method with ESI was applied for human plasma.http://www.jastmag.org/journal/view.php?Type=C&number=72Ultra High ResolutionMass SpectrometryMALDIESIFTICRMultiple Reaction MonitoringGlycoproteinCancer BiomarkerHuman Plasma |
| spellingShingle | Yeong Hee Ahn Kwang Hoe Kim Park Min Shin Eun Sun Ji Jong Shin Yoo Mass Spectrometry for Analysis of Cancer Biomarker from Human Plasma Journal of Analytical Science and Technology Ultra High Resolution Mass Spectrometry MALDI ESI FTICR Multiple Reaction Monitoring Glycoprotein Cancer Biomarker Human Plasma |
| title | Mass Spectrometry for Analysis of Cancer Biomarker from Human Plasma |
| title_full | Mass Spectrometry for Analysis of Cancer Biomarker from Human Plasma |
| title_fullStr | Mass Spectrometry for Analysis of Cancer Biomarker from Human Plasma |
| title_full_unstemmed | Mass Spectrometry for Analysis of Cancer Biomarker from Human Plasma |
| title_short | Mass Spectrometry for Analysis of Cancer Biomarker from Human Plasma |
| title_sort | mass spectrometry for analysis of cancer biomarker from human plasma |
| topic | Ultra High Resolution Mass Spectrometry MALDI ESI FTICR Multiple Reaction Monitoring Glycoprotein Cancer Biomarker Human Plasma |
| url | http://www.jastmag.org/journal/view.php?Type=C&number=72 |
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