Protocol to quantify and characterize contact between T cells and antigen-presenting cells in the antigen-draining lymph nodes of mice using flow cytometry

Protocol to quantify and characterize contact between T cells and antigen-presenting cells in the antigen-draining lymph nodes of mice using flow cytometry

Summary: Physical contact between T cells and antigen-presenting cells (APCs) is essential for priming antigen-specific T cells, but quantitating the antigen-dependent T cell-APC contact can be laborious. Here, we present a simple flow-cytometry-based protocol for quantitating T cell-APC contacts in...

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Bibliographic Details
Main Authors: Naoya Tatsumi, Alejandro Davila-Pagan, Yosuke Kumamoto
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Flow Cytometry/Mass Cytometry
Immunology
Microbiology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722007250

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