Electroless Palladium-Coated Polymer Scaffolds for Electrical Stimulation of Osteoblast-Like Saos-2 Cells
Three-dimensional porous scaffolds offer some advantages over conventional treatments for bone tissue engineering. Amongst all non-bioresorbable scaffolds, biocompatible metallic scaffolds are preferred over ceramic and polymeric scaffolds, as they can be used as electrodes with different electric f...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/2/528 |
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| author | Oriol Careta Asier Salicio-Paz Eva Pellicer Elena Ibáñez Jordina Fornell Eva García-Lecina Jordi Sort Carme Nogués |
| author_facet | Oriol Careta Asier Salicio-Paz Eva Pellicer Elena Ibáñez Jordina Fornell Eva García-Lecina Jordi Sort Carme Nogués |
| author_sort | Oriol Careta |
| collection | DOAJ |
| description | Three-dimensional porous scaffolds offer some advantages over conventional treatments for bone tissue engineering. Amongst all non-bioresorbable scaffolds, biocompatible metallic scaffolds are preferred over ceramic and polymeric scaffolds, as they can be used as electrodes with different electric field intensities (or voltages) for electric stimulation (ES). In the present work we have used a palladium-coated polymeric scaffold, generated by electroless deposition, as a bipolar electrode to electrically stimulate human osteoblast-like Saos-2 cells. Cells grown on palladium-coated polyurethane foams under ES presented higher proliferation than cells grown on foams without ES for up to 14 days. In addition, cells grown in both conditions were well adhered, with a flat appearance and a typical actin cytoskeleton distribution. However, after 28 days in culture, cells without ES were filling the entire structure, while cells under ES appeared rounded and not well adhered, a sign of cell death onset. Regarding osteoblast differentiation, ES seems to enhance the expression of early expressed genes. The results suggest that palladium-coated polyurethane foams may be good candidates for osteoblast scaffolds and demonstrate that ES enhances osteoblast proliferation up to 14 days and upregulate expression genes related to extracellular matrix formation. |
| first_indexed | 2024-03-09T05:49:19Z |
| format | Article |
| id | doaj.art-a0eab9d783084a9e93475f1ed50fb77b |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T05:49:19Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a0eab9d783084a9e93475f1ed50fb77b2023-12-03T12:18:26ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-01-0122252810.3390/ijms22020528Electroless Palladium-Coated Polymer Scaffolds for Electrical Stimulation of Osteoblast-Like Saos-2 CellsOriol Careta0Asier Salicio-Paz1Eva Pellicer2Elena Ibáñez3Jordina Fornell4Eva García-Lecina5Jordi Sort6Carme Nogués7Departament de Biologia Cel·lular, Fisiologia i Immunologia, Universitat Autònoma de Barcelona, E-08193 Bellaterra (Cerdanyola del Vallès), SpainCIDETEC, Basque Research and Technology Alliance (BRTA), Paseo Miramón 196, E-20014 Donostia-San Sebastián, SpainDepartament de Física, Universitat Autònoma de Barcelona, E-08193 Bellaterra (Cerdanyola del Vallès), SpainDepartament de Biologia Cel·lular, Fisiologia i Immunologia, Universitat Autònoma de Barcelona, E-08193 Bellaterra (Cerdanyola del Vallès), SpainDepartament de Física, Universitat Autònoma de Barcelona, E-08193 Bellaterra (Cerdanyola del Vallès), SpainCIDETEC, Basque Research and Technology Alliance (BRTA), Paseo Miramón 196, E-20014 Donostia-San Sebastián, SpainDepartament de Física, Universitat Autònoma de Barcelona, E-08193 Bellaterra (Cerdanyola del Vallès), SpainDepartament de Biologia Cel·lular, Fisiologia i Immunologia, Universitat Autònoma de Barcelona, E-08193 Bellaterra (Cerdanyola del Vallès), SpainThree-dimensional porous scaffolds offer some advantages over conventional treatments for bone tissue engineering. Amongst all non-bioresorbable scaffolds, biocompatible metallic scaffolds are preferred over ceramic and polymeric scaffolds, as they can be used as electrodes with different electric field intensities (or voltages) for electric stimulation (ES). In the present work we have used a palladium-coated polymeric scaffold, generated by electroless deposition, as a bipolar electrode to electrically stimulate human osteoblast-like Saos-2 cells. Cells grown on palladium-coated polyurethane foams under ES presented higher proliferation than cells grown on foams without ES for up to 14 days. In addition, cells grown in both conditions were well adhered, with a flat appearance and a typical actin cytoskeleton distribution. However, after 28 days in culture, cells without ES were filling the entire structure, while cells under ES appeared rounded and not well adhered, a sign of cell death onset. Regarding osteoblast differentiation, ES seems to enhance the expression of early expressed genes. The results suggest that palladium-coated polyurethane foams may be good candidates for osteoblast scaffolds and demonstrate that ES enhances osteoblast proliferation up to 14 days and upregulate expression genes related to extracellular matrix formation.https://www.mdpi.com/1422-0067/22/2/528electrical stimulationosteoblastPd-coated polyurethane scaffoldgene expressiondifferentiation |
| spellingShingle | Oriol Careta Asier Salicio-Paz Eva Pellicer Elena Ibáñez Jordina Fornell Eva García-Lecina Jordi Sort Carme Nogués Electroless Palladium-Coated Polymer Scaffolds for Electrical Stimulation of Osteoblast-Like Saos-2 Cells International Journal of Molecular Sciences electrical stimulation osteoblast Pd-coated polyurethane scaffold gene expression differentiation |
| title | Electroless Palladium-Coated Polymer Scaffolds for Electrical Stimulation of Osteoblast-Like Saos-2 Cells |
| title_full | Electroless Palladium-Coated Polymer Scaffolds for Electrical Stimulation of Osteoblast-Like Saos-2 Cells |
| title_fullStr | Electroless Palladium-Coated Polymer Scaffolds for Electrical Stimulation of Osteoblast-Like Saos-2 Cells |
| title_full_unstemmed | Electroless Palladium-Coated Polymer Scaffolds for Electrical Stimulation of Osteoblast-Like Saos-2 Cells |
| title_short | Electroless Palladium-Coated Polymer Scaffolds for Electrical Stimulation of Osteoblast-Like Saos-2 Cells |
| title_sort | electroless palladium coated polymer scaffolds for electrical stimulation of osteoblast like saos 2 cells |
| topic | electrical stimulation osteoblast Pd-coated polyurethane scaffold gene expression differentiation |
| url | https://www.mdpi.com/1422-0067/22/2/528 |
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