Genotoxic and Cytotoxic Effects on the Immune Cells of the Freshwater Bivalve Dreissena polymorpha Exposed to the Environmental Neurotoxin BMAA
The environmental neurotoxin β-N-Methylamino-l-alanine (BMAA) has been pointed out to be involved in human neurodegenerative diseases. This molecule is known to be bioaccumulated by bivalves. However, little data about its toxic effects on freshwater mussels is available, particularly on the hemolym...
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MDPI AG
2018-03-01
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| Serie: | Toxins |
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| Accesso online: | http://www.mdpi.com/2072-6651/10/3/106 |
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| author | Alexandra Lepoutre Nadia Milliote Marc Bonnard Mélissa Palos-Ladeiro Damien Rioult Isabelle Bonnard Fanny Bastien Elisabeth Faassen Alain Geffard Emilie Lance |
| author_facet | Alexandra Lepoutre Nadia Milliote Marc Bonnard Mélissa Palos-Ladeiro Damien Rioult Isabelle Bonnard Fanny Bastien Elisabeth Faassen Alain Geffard Emilie Lance |
| author_sort | Alexandra Lepoutre |
| collection | DOAJ |
| description | The environmental neurotoxin β-N-Methylamino-l-alanine (BMAA) has been pointed out to be involved in human neurodegenerative diseases. This molecule is known to be bioaccumulated by bivalves. However, little data about its toxic effects on freshwater mussels is available, particularly on the hemolymphatic compartment and its hemocyte cells involved in various physiological processes such as immune defenses, digestion and excretion, tissue repair, and shell production. Here we exposed Dreissena polymorpha to dissolved BMAA, at the environmental concentration of 7.5 µg of /mussel/3 days, during 21 days followed by 14 days of depuration in clear water, with the objective of assessing the BMAA presence in the hemolymphatic compartment, as well as the impact of the hemocyte cells in terms of potential cytotoxicity, immunotoxicity, and genotoxiciy. Data showed that hemocytes were in contact with BMAA. The presence of BMAA in hemolymph did not induce significant effect on hemocytes phagocytosis activity. However, significant DNA damage on hemocytes occurred during the first week (days 3 and 8) of BMAA exposure, followed by an increase of hemocyte mortality after 2 weeks of exposure. Those effects might be an indirect consequence of the BMAA-induced oxidative stress in cells. However, DNA strand breaks and mortality did not persist during the entire exposure, despite the BMAA persistence in the hemolymph, suggesting potential induction of some DNA-repair mechanisms. |
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| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-04-14T06:24:30Z |
| publishDate | 2018-03-01 |
| publisher | MDPI AG |
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| series | Toxins |
| spelling | doaj.art-a0eafc6540d647d8b2e847f79d2d14a12022-12-22T02:07:53ZengMDPI AGToxins2072-66512018-03-0110310610.3390/toxins10030106toxins10030106Genotoxic and Cytotoxic Effects on the Immune Cells of the Freshwater Bivalve Dreissena polymorpha Exposed to the Environmental Neurotoxin BMAAAlexandra Lepoutre0Nadia Milliote1Marc Bonnard2Mélissa Palos-Ladeiro3Damien Rioult4Isabelle Bonnard5Fanny Bastien6Elisabeth Faassen7Alain Geffard8Emilie Lance9Université de Reims Champagne-Ardenne UMR-I 02 INERIS-URCA-ULH SEBIO Unité Stress Environnementaux et, BIOsurveillance des milieux aquatiques UFR Sciences Exactes et Naturelles, Campus du Moulin de la Housse, BP 1039 51687 Reims CEDEX, FranceUniversité de Reims Champagne-Ardenne UMR-I 02 INERIS-URCA-ULH SEBIO Unité Stress Environnementaux et, BIOsurveillance des milieux aquatiques UFR Sciences Exactes et Naturelles, Campus du Moulin de la Housse, BP 1039 51687 Reims CEDEX, FranceUniversité de Reims Champagne-Ardenne UMR-I 02 INERIS-URCA-ULH SEBIO Unité Stress Environnementaux et, BIOsurveillance des milieux aquatiques UFR Sciences Exactes et Naturelles, Campus du Moulin de la Housse, BP 1039 51687 Reims CEDEX, FranceUniversité de Reims Champagne-Ardenne UMR-I 02 INERIS-URCA-ULH SEBIO Unité Stress Environnementaux et, BIOsurveillance des milieux aquatiques UFR Sciences Exactes et Naturelles, Campus du Moulin de la Housse, BP 1039 51687 Reims CEDEX, FranceUniversité de Reims Champagne-Ardenne UMR-I 02 INERIS-URCA-ULH SEBIO Unité Stress Environnementaux et, BIOsurveillance des milieux aquatiques UFR Sciences Exactes et Naturelles, Campus du Moulin de la Housse, BP 1039 51687 Reims CEDEX, FranceUniversité de Reims Champagne-Ardenne UMR-I 02 INERIS-URCA-ULH SEBIO Unité Stress Environnementaux et, BIOsurveillance des milieux aquatiques UFR Sciences Exactes et Naturelles, Campus du Moulin de la Housse, BP 1039 51687 Reims CEDEX, FranceUniversité de Reims Champagne-Ardenne UMR-I 02 INERIS-URCA-ULH SEBIO Unité Stress Environnementaux et, BIOsurveillance des milieux aquatiques UFR Sciences Exactes et Naturelles, Campus du Moulin de la Housse, BP 1039 51687 Reims CEDEX, FranceRIKILT, Wageningen Research, Akkermaalsbos 2, 6708 WB Wageningen, The NetherlandsUniversité de Reims Champagne-Ardenne UMR-I 02 INERIS-URCA-ULH SEBIO Unité Stress Environnementaux et, BIOsurveillance des milieux aquatiques UFR Sciences Exactes et Naturelles, Campus du Moulin de la Housse, BP 1039 51687 Reims CEDEX, FranceUniversité de Reims Champagne-Ardenne UMR-I 02 INERIS-URCA-ULH SEBIO Unité Stress Environnementaux et, BIOsurveillance des milieux aquatiques UFR Sciences Exactes et Naturelles, Campus du Moulin de la Housse, BP 1039 51687 Reims CEDEX, FranceThe environmental neurotoxin β-N-Methylamino-l-alanine (BMAA) has been pointed out to be involved in human neurodegenerative diseases. This molecule is known to be bioaccumulated by bivalves. However, little data about its toxic effects on freshwater mussels is available, particularly on the hemolymphatic compartment and its hemocyte cells involved in various physiological processes such as immune defenses, digestion and excretion, tissue repair, and shell production. Here we exposed Dreissena polymorpha to dissolved BMAA, at the environmental concentration of 7.5 µg of /mussel/3 days, during 21 days followed by 14 days of depuration in clear water, with the objective of assessing the BMAA presence in the hemolymphatic compartment, as well as the impact of the hemocyte cells in terms of potential cytotoxicity, immunotoxicity, and genotoxiciy. Data showed that hemocytes were in contact with BMAA. The presence of BMAA in hemolymph did not induce significant effect on hemocytes phagocytosis activity. However, significant DNA damage on hemocytes occurred during the first week (days 3 and 8) of BMAA exposure, followed by an increase of hemocyte mortality after 2 weeks of exposure. Those effects might be an indirect consequence of the BMAA-induced oxidative stress in cells. However, DNA strand breaks and mortality did not persist during the entire exposure, despite the BMAA persistence in the hemolymph, suggesting potential induction of some DNA-repair mechanisms.http://www.mdpi.com/2072-6651/10/3/106BMAAhemocytesgenotoxicitycytotoxicityimmunotoxicityfreshwater bivalves |
| spellingShingle | Alexandra Lepoutre Nadia Milliote Marc Bonnard Mélissa Palos-Ladeiro Damien Rioult Isabelle Bonnard Fanny Bastien Elisabeth Faassen Alain Geffard Emilie Lance Genotoxic and Cytotoxic Effects on the Immune Cells of the Freshwater Bivalve Dreissena polymorpha Exposed to the Environmental Neurotoxin BMAA Toxins BMAA hemocytes genotoxicity cytotoxicity immunotoxicity freshwater bivalves |
| title | Genotoxic and Cytotoxic Effects on the Immune Cells of the Freshwater Bivalve Dreissena polymorpha Exposed to the Environmental Neurotoxin BMAA |
| title_full | Genotoxic and Cytotoxic Effects on the Immune Cells of the Freshwater Bivalve Dreissena polymorpha Exposed to the Environmental Neurotoxin BMAA |
| title_fullStr | Genotoxic and Cytotoxic Effects on the Immune Cells of the Freshwater Bivalve Dreissena polymorpha Exposed to the Environmental Neurotoxin BMAA |
| title_full_unstemmed | Genotoxic and Cytotoxic Effects on the Immune Cells of the Freshwater Bivalve Dreissena polymorpha Exposed to the Environmental Neurotoxin BMAA |
| title_short | Genotoxic and Cytotoxic Effects on the Immune Cells of the Freshwater Bivalve Dreissena polymorpha Exposed to the Environmental Neurotoxin BMAA |
| title_sort | genotoxic and cytotoxic effects on the immune cells of the freshwater bivalve dreissena polymorpha exposed to the environmental neurotoxin bmaa |
| topic | BMAA hemocytes genotoxicity cytotoxicity immunotoxicity freshwater bivalves |
| url | http://www.mdpi.com/2072-6651/10/3/106 |
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