Phytochemicals of Periploca aphylla Dcne. ameliorated streptozotocin-induced diabetes in rat
Abstract Background Periploca aphylla is used by local population and indigenous medicine practitioners as stomachic, tonic, antitumor, antiulcer, and for treatment of inflammatory disorders. The aim of this study was to evaluate antidiabetic effect of the extract of P. aphylla and to investigate an...
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| Format: | Article |
| Language: | English |
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Komiyama Printing Co. Ltd
2021-03-01
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| Series: | Environmental Health and Preventive Medicine |
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| Online Access: | https://doi.org/10.1186/s12199-021-00962-0 |
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| author | Umbreen Rashid Muhammad Rashid Khan |
| author_facet | Umbreen Rashid Muhammad Rashid Khan |
| author_sort | Umbreen Rashid |
| collection | DOAJ |
| description | Abstract Background Periploca aphylla is used by local population and indigenous medicine practitioners as stomachic, tonic, antitumor, antiulcer, and for treatment of inflammatory disorders. The aim of this study was to evaluate antidiabetic effect of the extract of P. aphylla and to investigate antioxidant and hypolipidemic activity in streptozotocin (STZ)-induced diabetic rats. Methods The present research was conducted to evaluate the antihyperglycemic potential of methanol extract of P. aphylla (PAM) and subfractions n-hexane (PAH), chloroform (PAC), ethyl acetate (PAE), n-butanol (PAB), and aqueous (PAA) in glucose-overloaded hyperglycemic Sprague-Dawley rats. Based on the efficacy, PAB (200 mg/kg and 400 mg/kg) was tested for its antidiabetic activity in STZ-induced diabetic rats. Diabetes was induced via intraperitoneal injection of STZ (55 mg/kg) in rat. Blood glucose values were taken weekly. HPLC-DAD analysis of PAB was carried out for the presence of various polyphenols. Results HPLC-DAD analysis of PAB recorded the presence of rutin, catechin, caffeic acid, and myricetin. Oral administration of PAB at doses of 200 and 400 mg/kg for 21 days significantly restored (P < 0.01) body weight (%) and relative liver and relative kidney weight of diabetic rats. Diabetic control rats showed significant elevation (P < 0.01) of AST, ALT, ALP, LDH, total cholesterol, triglycerides, LDL, creatinine, total bilirubin, and BUN while reduced (P < 0.01) level of glucose, total protein, albumin, insulin, and HDL in serum. Count of blood cells and hematological parameters were altered in diabetic rats. Further, glutathione peroxidase, catalase, superoxide dismutase, glutathione reductase, and total soluble protein concentration decreased while concentration of thiobarbituric acid reactive substances and percent DNA damages increased (P < 0.01) in liver and renal tissues of diabetic rats. Histopathological damage scores increased in liver and kidney tissues of diabetic rats. Intake of PAB (400 mg/kg) resulted in significant improvement (P < 0.01) of above parameters, and results were comparable to that of standard drug glibenclamide. Conclusion The result suggests the antihyperglycemic, antioxidant, and anti-inflammatory activities of PAB treatment in STZ-compelled diabetic rat. PAB might be used as new therapeutic agent in diabetic patients to manage diabetes and decrease the complications. |
| first_indexed | 2024-12-10T21:09:13Z |
| format | Article |
| id | doaj.art-a0eccfb9ef8a40028299374965055374 |
| institution | Directory Open Access Journal |
| issn | 1342-078X 1347-4715 |
| language | English |
| last_indexed | 2024-12-10T21:09:13Z |
| publishDate | 2021-03-01 |
| publisher | Komiyama Printing Co. Ltd |
| record_format | Article |
| series | Environmental Health and Preventive Medicine |
| spelling | doaj.art-a0eccfb9ef8a400282993749650553742022-12-22T01:33:32ZengKomiyama Printing Co. LtdEnvironmental Health and Preventive Medicine1342-078X1347-47152021-03-0126111410.1186/s12199-021-00962-0Phytochemicals of Periploca aphylla Dcne. ameliorated streptozotocin-induced diabetes in ratUmbreen Rashid0Muhammad Rashid Khan1Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam UniversityDepartment of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam UniversityAbstract Background Periploca aphylla is used by local population and indigenous medicine practitioners as stomachic, tonic, antitumor, antiulcer, and for treatment of inflammatory disorders. The aim of this study was to evaluate antidiabetic effect of the extract of P. aphylla and to investigate antioxidant and hypolipidemic activity in streptozotocin (STZ)-induced diabetic rats. Methods The present research was conducted to evaluate the antihyperglycemic potential of methanol extract of P. aphylla (PAM) and subfractions n-hexane (PAH), chloroform (PAC), ethyl acetate (PAE), n-butanol (PAB), and aqueous (PAA) in glucose-overloaded hyperglycemic Sprague-Dawley rats. Based on the efficacy, PAB (200 mg/kg and 400 mg/kg) was tested for its antidiabetic activity in STZ-induced diabetic rats. Diabetes was induced via intraperitoneal injection of STZ (55 mg/kg) in rat. Blood glucose values were taken weekly. HPLC-DAD analysis of PAB was carried out for the presence of various polyphenols. Results HPLC-DAD analysis of PAB recorded the presence of rutin, catechin, caffeic acid, and myricetin. Oral administration of PAB at doses of 200 and 400 mg/kg for 21 days significantly restored (P < 0.01) body weight (%) and relative liver and relative kidney weight of diabetic rats. Diabetic control rats showed significant elevation (P < 0.01) of AST, ALT, ALP, LDH, total cholesterol, triglycerides, LDL, creatinine, total bilirubin, and BUN while reduced (P < 0.01) level of glucose, total protein, albumin, insulin, and HDL in serum. Count of blood cells and hematological parameters were altered in diabetic rats. Further, glutathione peroxidase, catalase, superoxide dismutase, glutathione reductase, and total soluble protein concentration decreased while concentration of thiobarbituric acid reactive substances and percent DNA damages increased (P < 0.01) in liver and renal tissues of diabetic rats. Histopathological damage scores increased in liver and kidney tissues of diabetic rats. Intake of PAB (400 mg/kg) resulted in significant improvement (P < 0.01) of above parameters, and results were comparable to that of standard drug glibenclamide. Conclusion The result suggests the antihyperglycemic, antioxidant, and anti-inflammatory activities of PAB treatment in STZ-compelled diabetic rat. PAB might be used as new therapeutic agent in diabetic patients to manage diabetes and decrease the complications.https://doi.org/10.1186/s12199-021-00962-0AntidiabeticAntioxidantAnti-inflammatoryInsulinLipid profile |
| spellingShingle | Umbreen Rashid Muhammad Rashid Khan Phytochemicals of Periploca aphylla Dcne. ameliorated streptozotocin-induced diabetes in rat Environmental Health and Preventive Medicine Antidiabetic Antioxidant Anti-inflammatory Insulin Lipid profile |
| title | Phytochemicals of Periploca aphylla Dcne. ameliorated streptozotocin-induced diabetes in rat |
| title_full | Phytochemicals of Periploca aphylla Dcne. ameliorated streptozotocin-induced diabetes in rat |
| title_fullStr | Phytochemicals of Periploca aphylla Dcne. ameliorated streptozotocin-induced diabetes in rat |
| title_full_unstemmed | Phytochemicals of Periploca aphylla Dcne. ameliorated streptozotocin-induced diabetes in rat |
| title_short | Phytochemicals of Periploca aphylla Dcne. ameliorated streptozotocin-induced diabetes in rat |
| title_sort | phytochemicals of periploca aphylla dcne ameliorated streptozotocin induced diabetes in rat |
| topic | Antidiabetic Antioxidant Anti-inflammatory Insulin Lipid profile |
| url | https://doi.org/10.1186/s12199-021-00962-0 |
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