The RNA helicase UPF1 associates with mRNAs co-transcriptionally and is required for the release of mRNAs from gene loci
UPF1 is an RNA helicase that is required for nonsense-mediated mRNA decay (NMD) in eukaryotes, and the predominant view is that UPF1 mainly operates on the 3’UTRs of mRNAs that are directed for NMD in the cytoplasm. Here we offer evidence, obtained from Drosophila, that UPF1 constantly moves between...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2019-03-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/41444 |
| _version_ | 1811202640361553920 |
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| author | Anand K Singh Subhendu Roy Choudhury Sandip De Jie Zhang Stephen Kissane Vibha Dwivedi Preethi Ramanathan Marija Petric Luisa Orsini Daniel Hebenstreit Saverio Brogna |
| author_facet | Anand K Singh Subhendu Roy Choudhury Sandip De Jie Zhang Stephen Kissane Vibha Dwivedi Preethi Ramanathan Marija Petric Luisa Orsini Daniel Hebenstreit Saverio Brogna |
| author_sort | Anand K Singh |
| collection | DOAJ |
| description | UPF1 is an RNA helicase that is required for nonsense-mediated mRNA decay (NMD) in eukaryotes, and the predominant view is that UPF1 mainly operates on the 3’UTRs of mRNAs that are directed for NMD in the cytoplasm. Here we offer evidence, obtained from Drosophila, that UPF1 constantly moves between the nucleus and cytoplasm by a mechanism that requires its RNA helicase activity. UPF1 is associated, genome-wide, with nascent RNAs at most of the active Pol II transcription sites and at some Pol III-transcribed genes, as demonstrated microscopically on the polytene chromosomes of salivary glands and by ChIP-seq analysis in S2 cells. Intron recognition seems to interfere with association and translocation of UPF1 on nascent pre-mRNAs, and cells depleted of UPF1 show defects in the release of mRNAs from transcription sites and their export from the nucleus. |
| first_indexed | 2024-04-12T02:43:12Z |
| format | Article |
| id | doaj.art-a0f2a48b174048d6a6a433c6a5d586ca |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:43:12Z |
| publishDate | 2019-03-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-a0f2a48b174048d6a6a433c6a5d586ca2022-12-22T03:51:16ZengeLife Sciences Publications LtdeLife2050-084X2019-03-01810.7554/eLife.41444The RNA helicase UPF1 associates with mRNAs co-transcriptionally and is required for the release of mRNAs from gene lociAnand K Singh0https://orcid.org/0000-0001-6500-6727Subhendu Roy Choudhury1Sandip De2Jie Zhang3Stephen Kissane4Vibha Dwivedi5Preethi Ramanathan6Marija Petric7Luisa Orsini8Daniel Hebenstreit9https://orcid.org/0000-0003-0144-6728Saverio Brogna10https://orcid.org/0000-0001-7063-4381School of Biosciences, University of Birmingham, Birmingham, United KingdomSchool of Biosciences, University of Birmingham, Birmingham, United KingdomSchool of Biosciences, University of Birmingham, Birmingham, United KingdomLife Sciences, University of Warwick, Coventry, United KingdomSchool of Biosciences, University of Birmingham, Birmingham, United KingdomSchool of Biosciences, University of Birmingham, Birmingham, United KingdomSchool of Biosciences, University of Birmingham, Birmingham, United KingdomSchool of Biosciences, University of Birmingham, Birmingham, United KingdomSchool of Biosciences, University of Birmingham, Birmingham, United KingdomLife Sciences, University of Warwick, Coventry, United KingdomSchool of Biosciences, University of Birmingham, Birmingham, United KingdomUPF1 is an RNA helicase that is required for nonsense-mediated mRNA decay (NMD) in eukaryotes, and the predominant view is that UPF1 mainly operates on the 3’UTRs of mRNAs that are directed for NMD in the cytoplasm. Here we offer evidence, obtained from Drosophila, that UPF1 constantly moves between the nucleus and cytoplasm by a mechanism that requires its RNA helicase activity. UPF1 is associated, genome-wide, with nascent RNAs at most of the active Pol II transcription sites and at some Pol III-transcribed genes, as demonstrated microscopically on the polytene chromosomes of salivary glands and by ChIP-seq analysis in S2 cells. Intron recognition seems to interfere with association and translocation of UPF1 on nascent pre-mRNAs, and cells depleted of UPF1 show defects in the release of mRNAs from transcription sites and their export from the nucleus.https://elifesciences.org/articles/41444NMDTranscriptionmRNA exportsplicingpolytene chromosomesRNA helicases |
| spellingShingle | Anand K Singh Subhendu Roy Choudhury Sandip De Jie Zhang Stephen Kissane Vibha Dwivedi Preethi Ramanathan Marija Petric Luisa Orsini Daniel Hebenstreit Saverio Brogna The RNA helicase UPF1 associates with mRNAs co-transcriptionally and is required for the release of mRNAs from gene loci eLife NMD Transcription mRNA export splicing polytene chromosomes RNA helicases |
| title | The RNA helicase UPF1 associates with mRNAs co-transcriptionally and is required for the release of mRNAs from gene loci |
| title_full | The RNA helicase UPF1 associates with mRNAs co-transcriptionally and is required for the release of mRNAs from gene loci |
| title_fullStr | The RNA helicase UPF1 associates with mRNAs co-transcriptionally and is required for the release of mRNAs from gene loci |
| title_full_unstemmed | The RNA helicase UPF1 associates with mRNAs co-transcriptionally and is required for the release of mRNAs from gene loci |
| title_short | The RNA helicase UPF1 associates with mRNAs co-transcriptionally and is required for the release of mRNAs from gene loci |
| title_sort | rna helicase upf1 associates with mrnas co transcriptionally and is required for the release of mrnas from gene loci |
| topic | NMD Transcription mRNA export splicing polytene chromosomes RNA helicases |
| url | https://elifesciences.org/articles/41444 |
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