Differential expression of defense-related genes in <em>Sinapis alba</em> and <em>Brassica juncea</em> upon the infection of <em>Alternaria brassicae</em>
Alternaria black spot of rapeseed mustard caused by the necrotrophic fungal pathogen <em>Alternaria brassicae</em> is one of the most important plant diseases in India. A comparative study on defense gene induction was undertaken to explore the signaling mechanisms that govern the defens...
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| Format: | Article |
| Language: | English |
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Postgraduate Institute of Agriculture, University of Peradeniya
2016-07-01
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| Series: | Tropical Agricultural Research |
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| Online Access: | https://tar.sljol.info/articles/8161 |
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| author | N. M. C. Nayanakantha S. Rawat S. Ali A. Grover |
| author_facet | N. M. C. Nayanakantha S. Rawat S. Ali A. Grover |
| author_sort | N. M. C. Nayanakantha |
| collection | DOAJ |
| description | Alternaria black spot of rapeseed mustard caused by the necrotrophic fungal pathogen <em>Alternaria brassicae</em> is one of the most important plant diseases in India. A comparative study on defense gene induction was undertaken to explore the signaling mechanisms that govern the defense responses of susceptible <em>Brassica juncea</em> and moderately resistant Sinapis alba, belonging to the family Brassicaceae, to alternaria leaf spot disease. The expression of five selected defense-related genes viz., pathogenesis related (PR)-1, PR-2, PR-3, non-expresser of PR-1(NPR-1) and plant defensin (PDF1.2) was examined after inoculation of the seedlings of <em>B. juncea</em> and <em>S. alba</em>. Transcripts of all five defense-related genes accumulated at a greater level and earlier in S. alba than in B. juncea upon challenge inoculation with A. brassicae locally as well as systemically. Although PDF1.2 is known to be induced only by jasmonic acid (JA) and PR-1 by salicylic acid (SA) in the model plant Arabidopsis thaliana belonging to the family Brassicaceae, PDF1.2 was induced also by SA and PR-1 by JA in both B. juncea and S. alba in the present study. These results suggest that defense signaling pathways in <em>B. juncea</em> and <em>S. alba</em> are different from <em>A. thaliana,</em>. Although JA responsive genes are predominantly induced in most crop plants and model plants against necrotrophic fungal pathogens, both JA and SA responsive genes seem to play an important role in S. alba conferring some resistance against <em>A. brassicae</em>. |
| first_indexed | 2024-04-14T07:37:48Z |
| format | Article |
| id | doaj.art-a0f434a473ca452ca9d3b6e16e550018 |
| institution | Directory Open Access Journal |
| issn | 1016-1422 |
| language | English |
| last_indexed | 2024-04-14T07:37:48Z |
| publishDate | 2016-07-01 |
| publisher | Postgraduate Institute of Agriculture, University of Peradeniya |
| record_format | Article |
| series | Tropical Agricultural Research |
| spelling | doaj.art-a0f434a473ca452ca9d3b6e16e5500182022-12-22T02:05:38ZengPostgraduate Institute of Agriculture, University of PeradeniyaTropical Agricultural Research1016-14222016-07-0127212313610.4038/tar.v27i2.81616086Differential expression of defense-related genes in <em>Sinapis alba</em> and <em>Brassica juncea</em> upon the infection of <em>Alternaria brassicae</em>N. M. C. Nayanakantha0S. Rawat1S. Ali2A. Grover3Rubber Research Institute (Sri Lanka), Dartonfield, AgalawattaIndian Agricultural Research Institute, New Delhi (110012)Indian Agricultural Research Institute, New Delhi (110012)Indian Agricultural Research Institute, New Delhi (110012)Alternaria black spot of rapeseed mustard caused by the necrotrophic fungal pathogen <em>Alternaria brassicae</em> is one of the most important plant diseases in India. A comparative study on defense gene induction was undertaken to explore the signaling mechanisms that govern the defense responses of susceptible <em>Brassica juncea</em> and moderately resistant Sinapis alba, belonging to the family Brassicaceae, to alternaria leaf spot disease. The expression of five selected defense-related genes viz., pathogenesis related (PR)-1, PR-2, PR-3, non-expresser of PR-1(NPR-1) and plant defensin (PDF1.2) was examined after inoculation of the seedlings of <em>B. juncea</em> and <em>S. alba</em>. Transcripts of all five defense-related genes accumulated at a greater level and earlier in S. alba than in B. juncea upon challenge inoculation with A. brassicae locally as well as systemically. Although PDF1.2 is known to be induced only by jasmonic acid (JA) and PR-1 by salicylic acid (SA) in the model plant Arabidopsis thaliana belonging to the family Brassicaceae, PDF1.2 was induced also by SA and PR-1 by JA in both B. juncea and S. alba in the present study. These results suggest that defense signaling pathways in <em>B. juncea</em> and <em>S. alba</em> are different from <em>A. thaliana,</em>. Although JA responsive genes are predominantly induced in most crop plants and model plants against necrotrophic fungal pathogens, both JA and SA responsive genes seem to play an important role in S. alba conferring some resistance against <em>A. brassicae</em>.https://tar.sljol.info/articles/8161brassica juncea, defense gene, jasmonic acid, salicylic acid, sinapis alba |
| spellingShingle | N. M. C. Nayanakantha S. Rawat S. Ali A. Grover Differential expression of defense-related genes in <em>Sinapis alba</em> and <em>Brassica juncea</em> upon the infection of <em>Alternaria brassicae</em> Tropical Agricultural Research brassica juncea, defense gene, jasmonic acid, salicylic acid, sinapis alba |
| title | Differential expression of defense-related genes in <em>Sinapis alba</em> and <em>Brassica juncea</em> upon the infection of <em>Alternaria brassicae</em> |
| title_full | Differential expression of defense-related genes in <em>Sinapis alba</em> and <em>Brassica juncea</em> upon the infection of <em>Alternaria brassicae</em> |
| title_fullStr | Differential expression of defense-related genes in <em>Sinapis alba</em> and <em>Brassica juncea</em> upon the infection of <em>Alternaria brassicae</em> |
| title_full_unstemmed | Differential expression of defense-related genes in <em>Sinapis alba</em> and <em>Brassica juncea</em> upon the infection of <em>Alternaria brassicae</em> |
| title_short | Differential expression of defense-related genes in <em>Sinapis alba</em> and <em>Brassica juncea</em> upon the infection of <em>Alternaria brassicae</em> |
| title_sort | differential expression of defense related genes in em sinapis alba em and em brassica juncea em upon the infection of em alternaria brassicae em |
| topic | brassica juncea, defense gene, jasmonic acid, salicylic acid, sinapis alba |
| url | https://tar.sljol.info/articles/8161 |
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