Partial Purification of Antimicrobial Compounds Isolated from Mycelia of Tropical Lentinus cladopus LC4
Lentinus cladopus LC4 produced at least eight antimicrobial compounds (ACs) which are active against plant and human pathogens. Three ACs in its crude mycelial were extracted with methanol and partial purification was carried out with silicic acid column chromatography and by thin layer chromatograp...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2010-06-01
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| Series: | Hayati Journal of Biosciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1978301916302121 |
| _version_ | 1818850985082093568 |
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| author | LISDAR IDWAN SUDIRMAN |
| author_facet | LISDAR IDWAN SUDIRMAN |
| author_sort | LISDAR IDWAN SUDIRMAN |
| collection | DOAJ |
| description | Lentinus cladopus LC4 produced at least eight antimicrobial compounds (ACs) which are active against plant and human pathogens. Three ACs in its crude mycelial were extracted with methanol and partial purification was carried out with silicic acid column chromatography and by thin layer chromatography (PTLC). The antimicrobial activity was tested by paper disc method and antibiographic method. The chromatography purification eluted with dichloromethane containing 5% methanol gave one active fraction (FII). This fraction which was active against X. campestris pv. glycines and showing two inhibition zones against Bacillus subtilis on bioautographic plates with the Rfs 0.8 and 0.7. FI and FIII fractions eluted with dichloromethane containing 0 and 10% methanol performed one inhibition zone with Rfs 0.8 and 0.7 respectively. However, their activities were lower than that of FII fraction. The PLTC purification gave one separate fraction with Rf value of 0.73 and it was active against X. campestris pv. glycines. The compound of Rf 0.73 fraction should be further studied using TLC and HPLC to obtain the pure substance for molecule characterization. |
| first_indexed | 2024-12-19T06:57:49Z |
| format | Article |
| id | doaj.art-a0f78d7fe2484dfa8285026b835f8026 |
| institution | Directory Open Access Journal |
| issn | 1978-3019 |
| language | English |
| last_indexed | 2024-12-19T06:57:49Z |
| publishDate | 2010-06-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-a0f78d7fe2484dfa8285026b835f80262022-12-21T20:31:29ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192010-06-01172636710.4308/hjb.17.2.63Partial Purification of Antimicrobial Compounds Isolated from Mycelia of Tropical Lentinus cladopus LC4LISDAR IDWAN SUDIRMANLentinus cladopus LC4 produced at least eight antimicrobial compounds (ACs) which are active against plant and human pathogens. Three ACs in its crude mycelial were extracted with methanol and partial purification was carried out with silicic acid column chromatography and by thin layer chromatography (PTLC). The antimicrobial activity was tested by paper disc method and antibiographic method. The chromatography purification eluted with dichloromethane containing 5% methanol gave one active fraction (FII). This fraction which was active against X. campestris pv. glycines and showing two inhibition zones against Bacillus subtilis on bioautographic plates with the Rfs 0.8 and 0.7. FI and FIII fractions eluted with dichloromethane containing 0 and 10% methanol performed one inhibition zone with Rfs 0.8 and 0.7 respectively. However, their activities were lower than that of FII fraction. The PLTC purification gave one separate fraction with Rf value of 0.73 and it was active against X. campestris pv. glycines. The compound of Rf 0.73 fraction should be further studied using TLC and HPLC to obtain the pure substance for molecule characterization.http://www.sciencedirect.com/science/article/pii/S1978301916302121tropical Lentinus cladopus LC4antimicrobial compoundspartial purification |
| spellingShingle | LISDAR IDWAN SUDIRMAN Partial Purification of Antimicrobial Compounds Isolated from Mycelia of Tropical Lentinus cladopus LC4 Hayati Journal of Biosciences tropical Lentinus cladopus LC4 antimicrobial compounds partial purification |
| title | Partial Purification of Antimicrobial Compounds Isolated from Mycelia of Tropical Lentinus cladopus LC4 |
| title_full | Partial Purification of Antimicrobial Compounds Isolated from Mycelia of Tropical Lentinus cladopus LC4 |
| title_fullStr | Partial Purification of Antimicrobial Compounds Isolated from Mycelia of Tropical Lentinus cladopus LC4 |
| title_full_unstemmed | Partial Purification of Antimicrobial Compounds Isolated from Mycelia of Tropical Lentinus cladopus LC4 |
| title_short | Partial Purification of Antimicrobial Compounds Isolated from Mycelia of Tropical Lentinus cladopus LC4 |
| title_sort | partial purification of antimicrobial compounds isolated from mycelia of tropical lentinus cladopus lc4 |
| topic | tropical Lentinus cladopus LC4 antimicrobial compounds partial purification |
| url | http://www.sciencedirect.com/science/article/pii/S1978301916302121 |
| work_keys_str_mv | AT lisdaridwansudirman partialpurificationofantimicrobialcompoundsisolatedfrommyceliaoftropicallentinuscladopuslc4 |