DNA barcoding of spiders from agricultural fields
In the present study, DNA barcoding was used to assess the percentage accuracy of morphological base identification of spiders from the agriculture fields of district Layyah, Punjab, Pakistan. A total of 872 spiders were captured from June to August of 2017. All the collected spiders were brought to...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-07-01
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| Series: | Mitochondrial DNA. Part B. Resources |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/23802359.2019.1693283 |
| _version_ | 1827773211629060096 |
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| author | Hafiz Muhammad Tahir Muhammad Summer Sana Mehmood Sehrish Ashraf Sajida Naseem |
| author_facet | Hafiz Muhammad Tahir Muhammad Summer Sana Mehmood Sehrish Ashraf Sajida Naseem |
| author_sort | Hafiz Muhammad Tahir |
| collection | DOAJ |
| description | In the present study, DNA barcoding was used to assess the percentage accuracy of morphological base identification of spiders from the agriculture fields of district Layyah, Punjab, Pakistan. A total of 872 spiders were captured from June to August of 2017. All the collected spiders were brought to molecular laboratory at GC University Lahore, preserved in 95% ethanol and stored at −20 °C until the DNA extraction. Spiders were evaluated morphologically on the basis of different identification Keys and Catalogs. Morphological identification revealed the presence of 12 families, 29 genra and 49 species. To evaluate the authenticity of morphological identification, tissue samples of 96 specimens were sent to Canadian Center for Biodiversity and Genomics, University of Guelph, Canada. A 658-base pair sequence of COI (Cytochrome c Oxidase Subunit I) of 90 specimens was retrieved successfully, which confirmed the presence of 11 families, 25 genra and 47 species. On the basis of molecular results, all the misidentified specimens were then allotted the correct taxon. Overall accuracy of morphological based identification was 88%. It is concluded from the present study that morphological investigations to identify a spider, are satisfactory but to enhance the accuracy, pace and credibility of results, molecular technique like DNA barcoding is considerable. Furthermore, to magnify authenticity of evaluation of spiders, integrated barcoding- combination of molecular methods and conventional taxonomy- is compulsory. |
| first_indexed | 2024-03-11T13:18:48Z |
| format | Article |
| id | doaj.art-a0f807e03d3c48d7b67680dc6871c921 |
| institution | Directory Open Access Journal |
| issn | 2380-2359 |
| language | English |
| last_indexed | 2024-03-11T13:18:48Z |
| publishDate | 2019-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mitochondrial DNA. Part B. Resources |
| spelling | doaj.art-a0f807e03d3c48d7b67680dc6871c9212023-11-03T10:38:52ZengTaylor & Francis GroupMitochondrial DNA. Part B. Resources2380-23592019-07-01424144415110.1080/23802359.2019.16932831693283DNA barcoding of spiders from agricultural fieldsHafiz Muhammad Tahir0Muhammad Summer1Sana Mehmood2Sehrish AshrafSajida Naseem3Government College University LahoreGovernment College University LahoreGovernment College University LahoreUniversity of Education Lower Mall Campus LahoreIn the present study, DNA barcoding was used to assess the percentage accuracy of morphological base identification of spiders from the agriculture fields of district Layyah, Punjab, Pakistan. A total of 872 spiders were captured from June to August of 2017. All the collected spiders were brought to molecular laboratory at GC University Lahore, preserved in 95% ethanol and stored at −20 °C until the DNA extraction. Spiders were evaluated morphologically on the basis of different identification Keys and Catalogs. Morphological identification revealed the presence of 12 families, 29 genra and 49 species. To evaluate the authenticity of morphological identification, tissue samples of 96 specimens were sent to Canadian Center for Biodiversity and Genomics, University of Guelph, Canada. A 658-base pair sequence of COI (Cytochrome c Oxidase Subunit I) of 90 specimens was retrieved successfully, which confirmed the presence of 11 families, 25 genra and 47 species. On the basis of molecular results, all the misidentified specimens were then allotted the correct taxon. Overall accuracy of morphological based identification was 88%. It is concluded from the present study that morphological investigations to identify a spider, are satisfactory but to enhance the accuracy, pace and credibility of results, molecular technique like DNA barcoding is considerable. Furthermore, to magnify authenticity of evaluation of spiders, integrated barcoding- combination of molecular methods and conventional taxonomy- is compulsory.http://dx.doi.org/10.1080/23802359.2019.1693283spidersbarcodingagroecosystemscoi |
| spellingShingle | Hafiz Muhammad Tahir Muhammad Summer Sana Mehmood Sehrish Ashraf Sajida Naseem DNA barcoding of spiders from agricultural fields Mitochondrial DNA. Part B. Resources spiders barcoding agroecosystems coi |
| title | DNA barcoding of spiders from agricultural fields |
| title_full | DNA barcoding of spiders from agricultural fields |
| title_fullStr | DNA barcoding of spiders from agricultural fields |
| title_full_unstemmed | DNA barcoding of spiders from agricultural fields |
| title_short | DNA barcoding of spiders from agricultural fields |
| title_sort | dna barcoding of spiders from agricultural fields |
| topic | spiders barcoding agroecosystems coi |
| url | http://dx.doi.org/10.1080/23802359.2019.1693283 |
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