Streptomyces catenulae as a Novel Marine Actinobacterium Mediated Silver Nanoparticles: Characterization, Biological Activities, and Proposed Mechanism of Antibacterial Action
Biosynthesized silver nanoparticles (Bio-SNPs) were synthesized from the marine actinobacterium strain Streptomyces catenulae M2 and characterized using a variety of techniques, including UV–vis spectrum, fourier transform infrared spectroscopy (FTIR), energy dispersive x-ray (EDX), transmission ele...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.833154/full |
| _version_ | 1811303945570615296 |
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| author | Maha A. Khalil Maha A. Khalil Abd El-Raheem R. El-Shanshoury Maha A. Alghamdi Maha A. Alghamdi Jianzhong Sun Sameh S. Ali Sameh S. Ali |
| author_facet | Maha A. Khalil Maha A. Khalil Abd El-Raheem R. El-Shanshoury Maha A. Alghamdi Maha A. Alghamdi Jianzhong Sun Sameh S. Ali Sameh S. Ali |
| author_sort | Maha A. Khalil |
| collection | DOAJ |
| description | Biosynthesized silver nanoparticles (Bio-SNPs) were synthesized from the marine actinobacterium strain Streptomyces catenulae M2 and characterized using a variety of techniques, including UV–vis spectrum, fourier transform infrared spectroscopy (FTIR), energy dispersive x-ray (EDX), transmission electron microscopy (TEM), dynamic light scattering (DLS), surface-enhanced Raman spectroscopy (SERS), and zeta potential. The antibacterial activity of Bio-SNPs alone and in combination with antibiotic was evaluated using a microtiter-dilution resazurin assay against multidrug-resistant (MDR) bacteria. Bio-SNPs’ minimum inhibitory concentration (MIC) against bacterial strains was determined. To assess the synergistic effect of Bio-SNPs in combination with antibiotics, the Fractional Inhibitory Concentration Index (FICI) was calculated. While the safety of Bio-SNPs in biomedical applications is dependent on their use, the in vitro cytotoxicity of Bio-SNPs on normal human epithelial colon cells (NCM460) and human colorectal adenocarcinoma cells (CaCo2) were evaluated using the [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) assay and cell lactate dehydrogenase (LDH) release. The presence of Bio-SNPs was revealed by UV–vis spectroscopy, which revealed a peak in the Surface Plasmon Resonance (SPR) spectrum at 439.5 nm. Bio-SNPs were spherical in shape and small in size (average 33 nm by TEM, 58.8 nm by DLS), with good stability (−30 mV) and the presence of capping agents. Bio-SNPs had MIC values ranging from 2 to 64 μg/ml against the bacteria tested. The MIC for P. aeruginosa was the lowest (2 μg/ml). Antibiotics have been shown to have a significant synergistic effect when combined with Bio-SNPs against tested bacteria. Bio-SNPs exhibited dose-dependent cytotoxicity against NCM460 and CaCo2 cancer cells, with the latter exhibiting far greater toxicity than the former. NCM460 and CaCo2 cell viability decreased from 99.3 to 95.7% and 92.3 to 61.8%, respectively, whereas LDH leakage increased from 200 to 215 nmol/ml and 261 to 730 nmol/ml, respectively. The half inhibitory concentrations (IC50) for NCM460 and CaCo2 cancer cells were 79.46 and 10.41 μg/ml and 89.4 and 19.3 μg/ml, respectively. Bio-SNPs were found to be biocompatible and to have anti-inflammatory activity. Bio-SNPs are highly appealing for future nanomedicine applications due to their antibacterial and biocompatible properties and their inherent “green” and simple manufacturing. |
| first_indexed | 2024-04-13T07:57:03Z |
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| id | doaj.art-a0f82d5c91114e33b386a8ff3f07fcb4 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-13T07:57:03Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-a0f82d5c91114e33b386a8ff3f07fcb42022-12-22T02:55:22ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-04-011310.3389/fmicb.2022.833154833154Streptomyces catenulae as a Novel Marine Actinobacterium Mediated Silver Nanoparticles: Characterization, Biological Activities, and Proposed Mechanism of Antibacterial ActionMaha A. Khalil0Maha A. Khalil1Abd El-Raheem R. El-Shanshoury2Maha A. Alghamdi3Maha A. Alghamdi4Jianzhong Sun5Sameh S. Ali6Sameh S. Ali7Department of Biology, College of Science, Taif University, Taif, Saudi ArabiaBotany and Microbiology Department, Faculty of Science, Tanta University, Tanta, EgyptBotany and Microbiology Department, Faculty of Science, Tanta University, Tanta, EgyptDepartment of Biotechnology, College of Science, Taif University, Taif, Saudi ArabiaDepartment of Molecular Medicine, Princess Al-Jawhara Centre for Molecular Medicine, School of Medicine and Medical Sciences, Arabian Gulf University, Manama, BahrainBiofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, ChinaBotany and Microbiology Department, Faculty of Science, Tanta University, Tanta, EgyptBiofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, ChinaBiosynthesized silver nanoparticles (Bio-SNPs) were synthesized from the marine actinobacterium strain Streptomyces catenulae M2 and characterized using a variety of techniques, including UV–vis spectrum, fourier transform infrared spectroscopy (FTIR), energy dispersive x-ray (EDX), transmission electron microscopy (TEM), dynamic light scattering (DLS), surface-enhanced Raman spectroscopy (SERS), and zeta potential. The antibacterial activity of Bio-SNPs alone and in combination with antibiotic was evaluated using a microtiter-dilution resazurin assay against multidrug-resistant (MDR) bacteria. Bio-SNPs’ minimum inhibitory concentration (MIC) against bacterial strains was determined. To assess the synergistic effect of Bio-SNPs in combination with antibiotics, the Fractional Inhibitory Concentration Index (FICI) was calculated. While the safety of Bio-SNPs in biomedical applications is dependent on their use, the in vitro cytotoxicity of Bio-SNPs on normal human epithelial colon cells (NCM460) and human colorectal adenocarcinoma cells (CaCo2) were evaluated using the [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (MTT) assay and cell lactate dehydrogenase (LDH) release. The presence of Bio-SNPs was revealed by UV–vis spectroscopy, which revealed a peak in the Surface Plasmon Resonance (SPR) spectrum at 439.5 nm. Bio-SNPs were spherical in shape and small in size (average 33 nm by TEM, 58.8 nm by DLS), with good stability (−30 mV) and the presence of capping agents. Bio-SNPs had MIC values ranging from 2 to 64 μg/ml against the bacteria tested. The MIC for P. aeruginosa was the lowest (2 μg/ml). Antibiotics have been shown to have a significant synergistic effect when combined with Bio-SNPs against tested bacteria. Bio-SNPs exhibited dose-dependent cytotoxicity against NCM460 and CaCo2 cancer cells, with the latter exhibiting far greater toxicity than the former. NCM460 and CaCo2 cell viability decreased from 99.3 to 95.7% and 92.3 to 61.8%, respectively, whereas LDH leakage increased from 200 to 215 nmol/ml and 261 to 730 nmol/ml, respectively. The half inhibitory concentrations (IC50) for NCM460 and CaCo2 cancer cells were 79.46 and 10.41 μg/ml and 89.4 and 19.3 μg/ml, respectively. Bio-SNPs were found to be biocompatible and to have anti-inflammatory activity. Bio-SNPs are highly appealing for future nanomedicine applications due to their antibacterial and biocompatible properties and their inherent “green” and simple manufacturing.https://www.frontiersin.org/articles/10.3389/fmicb.2022.833154/fullmarine actinobacteriasilver nanoparticlesantibacterialantioxidantanti-inflammatoryantitumor |
| spellingShingle | Maha A. Khalil Maha A. Khalil Abd El-Raheem R. El-Shanshoury Maha A. Alghamdi Maha A. Alghamdi Jianzhong Sun Sameh S. Ali Sameh S. Ali Streptomyces catenulae as a Novel Marine Actinobacterium Mediated Silver Nanoparticles: Characterization, Biological Activities, and Proposed Mechanism of Antibacterial Action Frontiers in Microbiology marine actinobacteria silver nanoparticles antibacterial antioxidant anti-inflammatory antitumor |
| title | Streptomyces catenulae as a Novel Marine Actinobacterium Mediated Silver Nanoparticles: Characterization, Biological Activities, and Proposed Mechanism of Antibacterial Action |
| title_full | Streptomyces catenulae as a Novel Marine Actinobacterium Mediated Silver Nanoparticles: Characterization, Biological Activities, and Proposed Mechanism of Antibacterial Action |
| title_fullStr | Streptomyces catenulae as a Novel Marine Actinobacterium Mediated Silver Nanoparticles: Characterization, Biological Activities, and Proposed Mechanism of Antibacterial Action |
| title_full_unstemmed | Streptomyces catenulae as a Novel Marine Actinobacterium Mediated Silver Nanoparticles: Characterization, Biological Activities, and Proposed Mechanism of Antibacterial Action |
| title_short | Streptomyces catenulae as a Novel Marine Actinobacterium Mediated Silver Nanoparticles: Characterization, Biological Activities, and Proposed Mechanism of Antibacterial Action |
| title_sort | streptomyces catenulae as a novel marine actinobacterium mediated silver nanoparticles characterization biological activities and proposed mechanism of antibacterial action |
| topic | marine actinobacteria silver nanoparticles antibacterial antioxidant anti-inflammatory antitumor |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.833154/full |
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