Long noncoding RNAs APOA1-AS, IFNG-AS1, RMRP and their related biomolecules in Egyptian patients with relapsing-remitting multiple sclerosis: Relation to disease activity and patient disability
Lately, long noncoding (lnc) RNAs are increasingly appreciated for their involvement in multiple sclerosis (MS). In inflammation and autoimmunity, a role of apoprotein A1 (ApoA1), mediated by sphingosine 1-phosphate receptors (S1PRs), was reported. However, the epigenetic mechanisms regulating these...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-01-01
|
| Series: | Journal of Advanced Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S209012321930178X |
| _version_ | 1828866047329959936 |
|---|---|
| author | Heba R. Ghaiad Alaa N. Elmazny Mohammed M. Nooh Maha M. El-Sawalhi Amira A. Shaheen |
| author_facet | Heba R. Ghaiad Alaa N. Elmazny Mohammed M. Nooh Maha M. El-Sawalhi Amira A. Shaheen |
| author_sort | Heba R. Ghaiad |
| collection | DOAJ |
| description | Lately, long noncoding (lnc) RNAs are increasingly appreciated for their involvement in multiple sclerosis (MS). In inflammation and autoimmunity, a role of apoprotein A1 (ApoA1), mediated by sphingosine 1-phosphate receptors (S1PRs), was reported. However, the epigenetic mechanisms regulating these biomolecules and their role in MS remains elusive. This case control study investigated the role of ApoA1, sphingosine kinase 1 and 2 (SPHK1 & 2), S1PR1 & 5, interferon-γ (IFN-γ) and interleukin 17 (IL17) in MS, beside three lncRNA: APOA1-AS, IFNG-AS1, and RMRP. Expression of SPHKs, S1PRs, and lncRNAs were measured in 72 relapsing-remitting MS patients (37 during relapse and 35 in remission) and 28 controls. Plasma levels of ApoA1, IFN-γ and IL17 were determined. The impact of these parameters on MS activity, relapse rate and patient disability was assessed. APOA1-AS, IFNG-AS1, SPHK1 & 2, and S1PR5 were upregulated in RRMS patients. Differences in ApoA1, SPHK2, and IL17 were observed between relapse and remission. Importantly, ApoA1, SPHK2, and IL17 were related to activity, while S1PR1 and IFN-γ were linked to disability, though, only IFN-γ was associated with relapse rate. Finally, an excellent diagnostic power of IFN-γ, IL17, SPHK1 and APOA1-AS was demonstrated, whereas SPHK2 showed promising prognostic power in predicting relapses. Keywords: Multiple sclerosis, LncRNA, Apoprotein A1, Interferon-γ, Sphingosine 1-phosphate receptors |
| first_indexed | 2024-12-13T04:42:03Z |
| format | Article |
| id | doaj.art-a0f9b82abefb40aea301d136248686f1 |
| institution | Directory Open Access Journal |
| issn | 2090-1232 |
| language | English |
| last_indexed | 2024-12-13T04:42:03Z |
| publishDate | 2020-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Advanced Research |
| spelling | doaj.art-a0f9b82abefb40aea301d136248686f12022-12-21T23:59:18ZengElsevierJournal of Advanced Research2090-12322020-01-0121141150Long noncoding RNAs APOA1-AS, IFNG-AS1, RMRP and their related biomolecules in Egyptian patients with relapsing-remitting multiple sclerosis: Relation to disease activity and patient disabilityHeba R. Ghaiad0Alaa N. Elmazny1Mohammed M. Nooh2Maha M. El-Sawalhi3Amira A. Shaheen4Biochemistry Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt; Corresponding author at: Biochemistry Department, Faculty of Pharmacy, Cairo University, Kasr el Ainy st., 11562 Cairo, Egypt.Neurology Department, Faculty of Medicine, Cairo University, Cairo, EgyptBiochemistry Department, Faculty of Pharmacy, Cairo University, Cairo, EgyptBiochemistry Department, Faculty of Pharmacy, Cairo University, Cairo, EgyptBiochemistry Department, Faculty of Pharmacy, Cairo University, Cairo, EgyptLately, long noncoding (lnc) RNAs are increasingly appreciated for their involvement in multiple sclerosis (MS). In inflammation and autoimmunity, a role of apoprotein A1 (ApoA1), mediated by sphingosine 1-phosphate receptors (S1PRs), was reported. However, the epigenetic mechanisms regulating these biomolecules and their role in MS remains elusive. This case control study investigated the role of ApoA1, sphingosine kinase 1 and 2 (SPHK1 & 2), S1PR1 & 5, interferon-γ (IFN-γ) and interleukin 17 (IL17) in MS, beside three lncRNA: APOA1-AS, IFNG-AS1, and RMRP. Expression of SPHKs, S1PRs, and lncRNAs were measured in 72 relapsing-remitting MS patients (37 during relapse and 35 in remission) and 28 controls. Plasma levels of ApoA1, IFN-γ and IL17 were determined. The impact of these parameters on MS activity, relapse rate and patient disability was assessed. APOA1-AS, IFNG-AS1, SPHK1 & 2, and S1PR5 were upregulated in RRMS patients. Differences in ApoA1, SPHK2, and IL17 were observed between relapse and remission. Importantly, ApoA1, SPHK2, and IL17 were related to activity, while S1PR1 and IFN-γ were linked to disability, though, only IFN-γ was associated with relapse rate. Finally, an excellent diagnostic power of IFN-γ, IL17, SPHK1 and APOA1-AS was demonstrated, whereas SPHK2 showed promising prognostic power in predicting relapses. Keywords: Multiple sclerosis, LncRNA, Apoprotein A1, Interferon-γ, Sphingosine 1-phosphate receptorshttp://www.sciencedirect.com/science/article/pii/S209012321930178X |
| spellingShingle | Heba R. Ghaiad Alaa N. Elmazny Mohammed M. Nooh Maha M. El-Sawalhi Amira A. Shaheen Long noncoding RNAs APOA1-AS, IFNG-AS1, RMRP and their related biomolecules in Egyptian patients with relapsing-remitting multiple sclerosis: Relation to disease activity and patient disability Journal of Advanced Research |
| title | Long noncoding RNAs APOA1-AS, IFNG-AS1, RMRP and their related biomolecules in Egyptian patients with relapsing-remitting multiple sclerosis: Relation to disease activity and patient disability |
| title_full | Long noncoding RNAs APOA1-AS, IFNG-AS1, RMRP and their related biomolecules in Egyptian patients with relapsing-remitting multiple sclerosis: Relation to disease activity and patient disability |
| title_fullStr | Long noncoding RNAs APOA1-AS, IFNG-AS1, RMRP and their related biomolecules in Egyptian patients with relapsing-remitting multiple sclerosis: Relation to disease activity and patient disability |
| title_full_unstemmed | Long noncoding RNAs APOA1-AS, IFNG-AS1, RMRP and their related biomolecules in Egyptian patients with relapsing-remitting multiple sclerosis: Relation to disease activity and patient disability |
| title_short | Long noncoding RNAs APOA1-AS, IFNG-AS1, RMRP and their related biomolecules in Egyptian patients with relapsing-remitting multiple sclerosis: Relation to disease activity and patient disability |
| title_sort | long noncoding rnas apoa1 as ifng as1 rmrp and their related biomolecules in egyptian patients with relapsing remitting multiple sclerosis relation to disease activity and patient disability |
| url | http://www.sciencedirect.com/science/article/pii/S209012321930178X |
| work_keys_str_mv | AT hebarghaiad longnoncodingrnasapoa1asifngas1rmrpandtheirrelatedbiomoleculesinegyptianpatientswithrelapsingremittingmultiplesclerosisrelationtodiseaseactivityandpatientdisability AT alaanelmazny longnoncodingrnasapoa1asifngas1rmrpandtheirrelatedbiomoleculesinegyptianpatientswithrelapsingremittingmultiplesclerosisrelationtodiseaseactivityandpatientdisability AT mohammedmnooh longnoncodingrnasapoa1asifngas1rmrpandtheirrelatedbiomoleculesinegyptianpatientswithrelapsingremittingmultiplesclerosisrelationtodiseaseactivityandpatientdisability AT mahamelsawalhi longnoncodingrnasapoa1asifngas1rmrpandtheirrelatedbiomoleculesinegyptianpatientswithrelapsingremittingmultiplesclerosisrelationtodiseaseactivityandpatientdisability AT amiraashaheen longnoncodingrnasapoa1asifngas1rmrpandtheirrelatedbiomoleculesinegyptianpatientswithrelapsingremittingmultiplesclerosisrelationtodiseaseactivityandpatientdisability |