Programmed Aptamer Screening, Characterization, and Rapid Detection for α-Conotoxin MI
Conotoxins (CTXs) are a variety of mixed polypeptide toxins, among which α-conotoxin MI (CTX-MI) is the most toxic. Serious toxic symptoms, a lack of counteracting drugs, and cumbersome detection processes have made CTX-MI a hidden danger for humans. One of the obstacles to resolving this problem is...
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MDPI AG
2022-10-01
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| Series: | Toxins |
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| Online Access: | https://www.mdpi.com/2072-6651/14/10/706 |
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| author | Han Guo Bowen Deng Luming Zhao Yun Gao Xiaojuan Zhang Chengfang Yang Bin Zou Han Chen Mingjuan Sun Lianghua Wang Binghua Jiao |
| author_facet | Han Guo Bowen Deng Luming Zhao Yun Gao Xiaojuan Zhang Chengfang Yang Bin Zou Han Chen Mingjuan Sun Lianghua Wang Binghua Jiao |
| author_sort | Han Guo |
| collection | DOAJ |
| description | Conotoxins (CTXs) are a variety of mixed polypeptide toxins, among which α-conotoxin MI (CTX-MI) is the most toxic. Serious toxic symptoms, a lack of counteracting drugs, and cumbersome detection processes have made CTX-MI a hidden danger for humans. One of the obstacles to resolving this problem is the absence of specific recognition elements. Aptamers have shown great advantages in the fields of molecule detection, drug development, etc. In this study, we screened and characterized aptamers for CTX-MI through a programmed process. MBMI-01c, the isolated aptamer, showed great affinity, with an affinity constant (K<sub>D</sub>) of 0.524 μM, and it formed an antiparallel G-quadruplet (GQ) structure for the specific recognition of CTX-MI. Additionally, an aptasensor based on the biolayer interferometry (BLI) platform was developed and displayed high precision, specificity, and repeatability with a limit of detection (LOD) of 0.26 μM. This aptasensor provides a potential tool for the rapid detection of CTX-MI in 10 min. The aptamer can be further developed for the enrichment, detoxification, and biological studies of CTX-MI. Additionally, the programmed process is applicable to screening and characterizing aptamers for other CTXs. |
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| id | doaj.art-a0fbb9cf9b4e42768e790d1583784a7e |
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| issn | 2072-6651 |
| language | English |
| last_indexed | 2024-03-09T19:25:05Z |
| publishDate | 2022-10-01 |
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| series | Toxins |
| spelling | doaj.art-a0fbb9cf9b4e42768e790d1583784a7e2023-11-24T02:59:28ZengMDPI AGToxins2072-66512022-10-01141070610.3390/toxins14100706Programmed Aptamer Screening, Characterization, and Rapid Detection for α-Conotoxin MIHan Guo0Bowen Deng1Luming Zhao2Yun Gao3Xiaojuan Zhang4Chengfang Yang5Bin Zou6Han Chen7Mingjuan Sun8Lianghua Wang9Binghua Jiao10Department of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Naval Medical University, Shanghai 200433, ChinaDepartment of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Naval Medical University, Shanghai 200433, ChinaDepartment of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Naval Medical University, Shanghai 200433, ChinaDepartment of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Naval Medical University, Shanghai 200433, ChinaDepartment of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Naval Medical University, Shanghai 200433, ChinaDepartment of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Naval Medical University, Shanghai 200433, ChinaDepartment of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Naval Medical University, Shanghai 200433, ChinaDepartment of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Naval Medical University, Shanghai 200433, ChinaDepartment of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Naval Medical University, Shanghai 200433, ChinaDepartment of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Naval Medical University, Shanghai 200433, ChinaDepartment of Biochemistry and Molecular Biology, College of Basic Medical Sciences, Naval Medical University, Shanghai 200433, ChinaConotoxins (CTXs) are a variety of mixed polypeptide toxins, among which α-conotoxin MI (CTX-MI) is the most toxic. Serious toxic symptoms, a lack of counteracting drugs, and cumbersome detection processes have made CTX-MI a hidden danger for humans. One of the obstacles to resolving this problem is the absence of specific recognition elements. Aptamers have shown great advantages in the fields of molecule detection, drug development, etc. In this study, we screened and characterized aptamers for CTX-MI through a programmed process. MBMI-01c, the isolated aptamer, showed great affinity, with an affinity constant (K<sub>D</sub>) of 0.524 μM, and it formed an antiparallel G-quadruplet (GQ) structure for the specific recognition of CTX-MI. Additionally, an aptasensor based on the biolayer interferometry (BLI) platform was developed and displayed high precision, specificity, and repeatability with a limit of detection (LOD) of 0.26 μM. This aptasensor provides a potential tool for the rapid detection of CTX-MI in 10 min. The aptamer can be further developed for the enrichment, detoxification, and biological studies of CTX-MI. Additionally, the programmed process is applicable to screening and characterizing aptamers for other CTXs.https://www.mdpi.com/2072-6651/14/10/706alpha-conotoxin MI (CTX-MI)magnetic beads SELEX (MB-SELEX)high-throughput sequencing (HTS)aptamercomputer simulationaptasensor |
| spellingShingle | Han Guo Bowen Deng Luming Zhao Yun Gao Xiaojuan Zhang Chengfang Yang Bin Zou Han Chen Mingjuan Sun Lianghua Wang Binghua Jiao Programmed Aptamer Screening, Characterization, and Rapid Detection for α-Conotoxin MI Toxins alpha-conotoxin MI (CTX-MI) magnetic beads SELEX (MB-SELEX) high-throughput sequencing (HTS) aptamer computer simulation aptasensor |
| title | Programmed Aptamer Screening, Characterization, and Rapid Detection for α-Conotoxin MI |
| title_full | Programmed Aptamer Screening, Characterization, and Rapid Detection for α-Conotoxin MI |
| title_fullStr | Programmed Aptamer Screening, Characterization, and Rapid Detection for α-Conotoxin MI |
| title_full_unstemmed | Programmed Aptamer Screening, Characterization, and Rapid Detection for α-Conotoxin MI |
| title_short | Programmed Aptamer Screening, Characterization, and Rapid Detection for α-Conotoxin MI |
| title_sort | programmed aptamer screening characterization and rapid detection for α conotoxin mi |
| topic | alpha-conotoxin MI (CTX-MI) magnetic beads SELEX (MB-SELEX) high-throughput sequencing (HTS) aptamer computer simulation aptasensor |
| url | https://www.mdpi.com/2072-6651/14/10/706 |
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