Biosynthesis of Zinc Oxide Nanoparticles using Intracellular Extract of Saccharomyces cerevisiae and Evaluation of its Antibacterial and Antioxidant Activities
Introduction: Attention to the biosynthesis of nanoparticles (NPs) has been increased recently since they are cost-effective, eco-friendly, and potential alternatives to chemical and physical methods. This study aimed to synthesize zinc oxide nanoparticles (ZnO NPs) using an intracellular extract of...
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| Format: | Article |
| Language: | fas |
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Ilam University of Medical Sciences
2020-10-01
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| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
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| Online Access: | http://sjimu.medilam.ac.ir/article-1-6343-en.html |
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| author | Razieh Motazedi Somayeh Rahaiee Mahboobeh Zare |
| author_facet | Razieh Motazedi Somayeh Rahaiee Mahboobeh Zare |
| author_sort | Razieh Motazedi |
| collection | DOAJ |
| description | Introduction: Attention to the biosynthesis of nanoparticles (NPs) has been increased recently since they are cost-effective, eco-friendly, and potential alternatives to chemical and physical methods. This study aimed to synthesize zinc oxide nanoparticles (ZnO NPs) using an intracellular extract of Saccharomyces cerevisiae. Moreover, it was attempted to evaluate their antibacterial and antioxidant effects.
Materials & Methods: After the preparation and identification of the physical characteristics of the ZnO NPs, their antioxidant activity was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Ferric Reducing Ability of Plasma (FRAP). Moreover, the antibacterial activity of NPs was tested against Gram-positive bacteria (Staphylococcus aureus and Listeria monocytogenes) and Gram-negative bacteria (Escherichia coli) using a disc diffusion method. Ethics code: IR.ausmt.rec.1398.11.33
Findings: The results showed that the synthesized NPs had a spherical shape, and their diameter size was < 30 nm. A good absorption at 370 nm confirmed the presence of ZnO NPs. These NPs depicted an improved antibacterial activity against S. aureus. Moreover, they showed concentration-dependent antioxidant activity in both DPPH and FRAP.
Discussions & Conclusions: The results indicated that the biosynthesized ZnO NPs had antibacterial and antioxidant activities. This suggests that ZnO NPs can be used in food packaging and cosmetic products. In addition, they can be utilized as an alternative to synthetic antibiotics. However, further studies are required to be conducted in this regard. |
| first_indexed | 2024-12-14T00:23:59Z |
| format | Article |
| id | doaj.art-a0fc075d9b0f48f9a083bd5a52c720a9 |
| institution | Directory Open Access Journal |
| issn | 1563-4728 2588-3135 |
| language | fas |
| last_indexed | 2024-12-14T00:23:59Z |
| publishDate | 2020-10-01 |
| publisher | Ilam University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām |
| spelling | doaj.art-a0fc075d9b0f48f9a083bd5a52c720a92022-12-21T23:25:08ZfasIlam University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām1563-47282588-31352020-10-012843646Biosynthesis of Zinc Oxide Nanoparticles using Intracellular Extract of Saccharomyces cerevisiae and Evaluation of its Antibacterial and Antioxidant ActivitiesRazieh Motazedi0Somayeh Rahaiee1Mahboobeh Zare2 Dept of Microbial Biotechnology, Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran Dept of Microbial Biotechnology, Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran Dept of Medicinal Plant, Faculty of Medicinal Plants, Amol University of Special Modern Technologies, Amol, Iran Introduction: Attention to the biosynthesis of nanoparticles (NPs) has been increased recently since they are cost-effective, eco-friendly, and potential alternatives to chemical and physical methods. This study aimed to synthesize zinc oxide nanoparticles (ZnO NPs) using an intracellular extract of Saccharomyces cerevisiae. Moreover, it was attempted to evaluate their antibacterial and antioxidant effects. Materials & Methods: After the preparation and identification of the physical characteristics of the ZnO NPs, their antioxidant activity was determined using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Ferric Reducing Ability of Plasma (FRAP). Moreover, the antibacterial activity of NPs was tested against Gram-positive bacteria (Staphylococcus aureus and Listeria monocytogenes) and Gram-negative bacteria (Escherichia coli) using a disc diffusion method. Ethics code: IR.ausmt.rec.1398.11.33 Findings: The results showed that the synthesized NPs had a spherical shape, and their diameter size was < 30 nm. A good absorption at 370 nm confirmed the presence of ZnO NPs. These NPs depicted an improved antibacterial activity against S. aureus. Moreover, they showed concentration-dependent antioxidant activity in both DPPH and FRAP. Discussions & Conclusions: The results indicated that the biosynthesized ZnO NPs had antibacterial and antioxidant activities. This suggests that ZnO NPs can be used in food packaging and cosmetic products. In addition, they can be utilized as an alternative to synthetic antibiotics. However, further studies are required to be conducted in this regard.http://sjimu.medilam.ac.ir/article-1-6343-en.htmlzno npsbiosynthesissaccharomyces cerevisiaeantibacterialantioxidant |
| spellingShingle | Razieh Motazedi Somayeh Rahaiee Mahboobeh Zare Biosynthesis of Zinc Oxide Nanoparticles using Intracellular Extract of Saccharomyces cerevisiae and Evaluation of its Antibacterial and Antioxidant Activities Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Īlām zno nps biosynthesis saccharomyces cerevisiae antibacterial antioxidant |
| title | Biosynthesis of Zinc Oxide Nanoparticles using Intracellular
Extract of Saccharomyces cerevisiae and Evaluation of its Antibacterial and Antioxidant Activities |
| title_full | Biosynthesis of Zinc Oxide Nanoparticles using Intracellular
Extract of Saccharomyces cerevisiae and Evaluation of its Antibacterial and Antioxidant Activities |
| title_fullStr | Biosynthesis of Zinc Oxide Nanoparticles using Intracellular
Extract of Saccharomyces cerevisiae and Evaluation of its Antibacterial and Antioxidant Activities |
| title_full_unstemmed | Biosynthesis of Zinc Oxide Nanoparticles using Intracellular
Extract of Saccharomyces cerevisiae and Evaluation of its Antibacterial and Antioxidant Activities |
| title_short | Biosynthesis of Zinc Oxide Nanoparticles using Intracellular
Extract of Saccharomyces cerevisiae and Evaluation of its Antibacterial and Antioxidant Activities |
| title_sort | biosynthesis of zinc oxide nanoparticles using intracellular extract of saccharomyces cerevisiae and evaluation of its antibacterial and antioxidant activities |
| topic | zno nps biosynthesis saccharomyces cerevisiae antibacterial antioxidant |
| url | http://sjimu.medilam.ac.ir/article-1-6343-en.html |
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