Effect of Essential Oil from <i>Lippia origanoides</i> on the Transcriptional Expression of Genes Related to Quorum Sensing, Biofilm Formation, and Virulence of <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>
Microbial infections resistant to conventional antibiotics constitute one of the most important causes of mortality in the world. In some bacterial species, such as <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> pathogens, biofilm formation can favor their antimicro...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-05-01
|
| Series: | Antibiotics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6382/12/5/845 |
| _version_ | 1797601353577529344 |
|---|---|
| author | Andrés Martínez Elena E. Stashenko Rodrigo Torres Sáez German Zafra Claudia Ortiz |
| author_facet | Andrés Martínez Elena E. Stashenko Rodrigo Torres Sáez German Zafra Claudia Ortiz |
| author_sort | Andrés Martínez |
| collection | DOAJ |
| description | Microbial infections resistant to conventional antibiotics constitute one of the most important causes of mortality in the world. In some bacterial species, such as <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> pathogens, biofilm formation can favor their antimicrobial resistance. These biofilm-forming bacteria produce a compact and protective matrix, allowing their adherence and colonization to different surfaces, and contributing to resistance, recurrence, and chronicity of the infections. Therefore, different therapeutic alternatives have been investigated to interrupt both cellular communication routes and biofilm formation. Among these, essential oils (EO) from <i>Lippia origanoides</i> thymol-carvacrol II chemotype (LOTC II) plants have demonstrated biological activity against different biofilm-forming pathogenic bacteria. In this work, we determined the effect of LOTC II EO on the expression of genes associated with quorum sensing (QS) communication, biofilm formation, and virulence of <i>E. coli</i> ATCC 25922 and <i>S. aureus</i> ATCC 29213. This EO was found to have high efficacy against biofilm formation, decreasing—by negative regulation—the expression of genes involved in motility (<i>fimH</i>), adherence and cellular aggregation (<i>csgD</i>), and exopolysaccharide production (<i>pgaC</i>) in <i>E. coli</i>. In addition, this effect was also determined in <i>S. aureus</i> where the <i>L. origanoides</i> EO diminished the expression of genes involved in QS communication (<i>agrA</i>), production of exopolysaccharides by PIA/PNG (<i>icaA</i>), synthesis of alpha hemolysin (<i>hla</i>), transcriptional regulators of the production of extracellular toxins (RNA III), QS and biofilm formation transcriptional regulators (<i>sarA</i>) and global regulators of biofilm formation (<i>rbf</i> and <i>aur</i>). Positive regulation was observed on the expression of genes encoding inhibitors of biofilm formation (e.g., <i>sdiA</i> and <i>ariR</i>). These findings suggest that LOTCII EO can affect biological pathways associated with QS communication, biofilm formation, and virulence of <i>E. coli</i> and <i>S. aureus</i> at subinhibitory concentrations and could be a promising candidate as a natural antibacterial alternative to conventional antibiotics. |
| first_indexed | 2024-03-11T04:00:50Z |
| format | Article |
| id | doaj.art-a0fcc0cebc3542d8903913066d29aa69 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-11T04:00:50Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-a0fcc0cebc3542d8903913066d29aa692023-11-18T00:12:08ZengMDPI AGAntibiotics2079-63822023-05-0112584510.3390/antibiotics12050845Effect of Essential Oil from <i>Lippia origanoides</i> on the Transcriptional Expression of Genes Related to Quorum Sensing, Biofilm Formation, and Virulence of <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>Andrés Martínez0Elena E. Stashenko1Rodrigo Torres Sáez2German Zafra3Claudia Ortiz4Grupo de Investigación en Bioquímica y Microbiología (GIBIM), Escuela de Microbiología, Facultad de Salud, Universidad Industrial de Santander, Bucaramanga 680002, ColombiaEscuela de Química, Centro de Cromatografía y Espectrometría de Masas (CROM-MASS), Universidad Industrial de Santander, Bucaramanga 680002, ColombiaGrupo de Investigación en Bioquímica y Microbiología (GIBIM), Escuela de Microbiología, Facultad de Salud, Universidad Industrial de Santander, Bucaramanga 680002, ColombiaGrupo de Investigación en Bioquímica y Microbiología (GIBIM), Escuela de Microbiología, Facultad de Salud, Universidad Industrial de Santander, Bucaramanga 680002, ColombiaGrupo de Investigación en Bioquímica y Microbiología (GIBIM), Escuela de Microbiología, Facultad de Salud, Universidad Industrial de Santander, Bucaramanga 680002, ColombiaMicrobial infections resistant to conventional antibiotics constitute one of the most important causes of mortality in the world. In some bacterial species, such as <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> pathogens, biofilm formation can favor their antimicrobial resistance. These biofilm-forming bacteria produce a compact and protective matrix, allowing their adherence and colonization to different surfaces, and contributing to resistance, recurrence, and chronicity of the infections. Therefore, different therapeutic alternatives have been investigated to interrupt both cellular communication routes and biofilm formation. Among these, essential oils (EO) from <i>Lippia origanoides</i> thymol-carvacrol II chemotype (LOTC II) plants have demonstrated biological activity against different biofilm-forming pathogenic bacteria. In this work, we determined the effect of LOTC II EO on the expression of genes associated with quorum sensing (QS) communication, biofilm formation, and virulence of <i>E. coli</i> ATCC 25922 and <i>S. aureus</i> ATCC 29213. This EO was found to have high efficacy against biofilm formation, decreasing—by negative regulation—the expression of genes involved in motility (<i>fimH</i>), adherence and cellular aggregation (<i>csgD</i>), and exopolysaccharide production (<i>pgaC</i>) in <i>E. coli</i>. In addition, this effect was also determined in <i>S. aureus</i> where the <i>L. origanoides</i> EO diminished the expression of genes involved in QS communication (<i>agrA</i>), production of exopolysaccharides by PIA/PNG (<i>icaA</i>), synthesis of alpha hemolysin (<i>hla</i>), transcriptional regulators of the production of extracellular toxins (RNA III), QS and biofilm formation transcriptional regulators (<i>sarA</i>) and global regulators of biofilm formation (<i>rbf</i> and <i>aur</i>). Positive regulation was observed on the expression of genes encoding inhibitors of biofilm formation (e.g., <i>sdiA</i> and <i>ariR</i>). These findings suggest that LOTCII EO can affect biological pathways associated with QS communication, biofilm formation, and virulence of <i>E. coli</i> and <i>S. aureus</i> at subinhibitory concentrations and could be a promising candidate as a natural antibacterial alternative to conventional antibiotics.https://www.mdpi.com/2079-6382/12/5/845biofilmessential oils<i>Lippia origanoides</i>gene expression analyses<i>E. coli</i><i>S. aureus</i> |
| spellingShingle | Andrés Martínez Elena E. Stashenko Rodrigo Torres Sáez German Zafra Claudia Ortiz Effect of Essential Oil from <i>Lippia origanoides</i> on the Transcriptional Expression of Genes Related to Quorum Sensing, Biofilm Formation, and Virulence of <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> Antibiotics biofilm essential oils <i>Lippia origanoides</i> gene expression analyses <i>E. coli</i> <i>S. aureus</i> |
| title | Effect of Essential Oil from <i>Lippia origanoides</i> on the Transcriptional Expression of Genes Related to Quorum Sensing, Biofilm Formation, and Virulence of <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> |
| title_full | Effect of Essential Oil from <i>Lippia origanoides</i> on the Transcriptional Expression of Genes Related to Quorum Sensing, Biofilm Formation, and Virulence of <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> |
| title_fullStr | Effect of Essential Oil from <i>Lippia origanoides</i> on the Transcriptional Expression of Genes Related to Quorum Sensing, Biofilm Formation, and Virulence of <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> |
| title_full_unstemmed | Effect of Essential Oil from <i>Lippia origanoides</i> on the Transcriptional Expression of Genes Related to Quorum Sensing, Biofilm Formation, and Virulence of <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> |
| title_short | Effect of Essential Oil from <i>Lippia origanoides</i> on the Transcriptional Expression of Genes Related to Quorum Sensing, Biofilm Formation, and Virulence of <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> |
| title_sort | effect of essential oil from i lippia origanoides i on the transcriptional expression of genes related to quorum sensing biofilm formation and virulence of i escherichia coli i and i staphylococcus aureus i |
| topic | biofilm essential oils <i>Lippia origanoides</i> gene expression analyses <i>E. coli</i> <i>S. aureus</i> |
| url | https://www.mdpi.com/2079-6382/12/5/845 |
| work_keys_str_mv | AT andresmartinez effectofessentialoilfromilippiaoriganoidesionthetranscriptionalexpressionofgenesrelatedtoquorumsensingbiofilmformationandvirulenceofiescherichiacoliiandistaphylococcusaureusi AT elenaestashenko effectofessentialoilfromilippiaoriganoidesionthetranscriptionalexpressionofgenesrelatedtoquorumsensingbiofilmformationandvirulenceofiescherichiacoliiandistaphylococcusaureusi AT rodrigotorressaez effectofessentialoilfromilippiaoriganoidesionthetranscriptionalexpressionofgenesrelatedtoquorumsensingbiofilmformationandvirulenceofiescherichiacoliiandistaphylococcusaureusi AT germanzafra effectofessentialoilfromilippiaoriganoidesionthetranscriptionalexpressionofgenesrelatedtoquorumsensingbiofilmformationandvirulenceofiescherichiacoliiandistaphylococcusaureusi AT claudiaortiz effectofessentialoilfromilippiaoriganoidesionthetranscriptionalexpressionofgenesrelatedtoquorumsensingbiofilmformationandvirulenceofiescherichiacoliiandistaphylococcusaureusi |