Structural and biophysical characterization of the nucleosome-binding PZP domain
Summary: The core subunit of the MORF acetyltransferase complex BRPF1 contains a unique combination of zinc fingers, including a plant homeodomain (PHD) finger followed by a zinc knuckle and another PHD finger, which together form a PZP domain (BRPF1PZP). BRPF1PZP has been shown to bind to the nucle...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721001866 |
| _version_ | 1818657170901696512 |
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| author | Brianna J. Klein Khan L. Cox Suk Min Jang Rohit K. Singh Jacques Côté Michael G. Poirier Tatiana G. Kutateladze |
| author_facet | Brianna J. Klein Khan L. Cox Suk Min Jang Rohit K. Singh Jacques Côté Michael G. Poirier Tatiana G. Kutateladze |
| author_sort | Brianna J. Klein |
| collection | DOAJ |
| description | Summary: The core subunit of the MORF acetyltransferase complex BRPF1 contains a unique combination of zinc fingers, including a plant homeodomain (PHD) finger followed by a zinc knuckle and another PHD finger, which together form a PZP domain (BRPF1PZP). BRPF1PZP has been shown to bind to the nucleosome and make contacts with both histone H3 tail and DNA. Here, we describe biophysical and structural methods for characterization of the interactions between BRPF1PZP, H3 tail, DNA, and the intact nucleosome.For complete details on the use and execution of this protocol, please refer to Klein et al. (2020). |
| first_indexed | 2024-12-17T03:37:14Z |
| format | Article |
| id | doaj.art-a0fd143f19b94e7ea9ae4c1f09faf24e |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-17T03:37:14Z |
| publishDate | 2021-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-a0fd143f19b94e7ea9ae4c1f09faf24e2022-12-21T22:05:07ZengElsevierSTAR Protocols2666-16672021-06-0122100479Structural and biophysical characterization of the nucleosome-binding PZP domainBrianna J. Klein0Khan L. Cox1Suk Min Jang2Rohit K. Singh3Jacques Côté4Michael G. Poirier5Tatiana G. Kutateladze6Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, USADepartment of Physics, Ohio State University, Columbus, OH 43210, USALaval University Cancer Research Center, CHU de Québec-UL Research Center - Oncology Division, Quebec City, QC G1R 3S3, CanadaDepartment of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, USALaval University Cancer Research Center, CHU de Québec-UL Research Center - Oncology Division, Quebec City, QC G1R 3S3, CanadaDepartment of Physics, Ohio State University, Columbus, OH 43210, USA; Corresponding authorDepartment of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, USA; Corresponding authorSummary: The core subunit of the MORF acetyltransferase complex BRPF1 contains a unique combination of zinc fingers, including a plant homeodomain (PHD) finger followed by a zinc knuckle and another PHD finger, which together form a PZP domain (BRPF1PZP). BRPF1PZP has been shown to bind to the nucleosome and make contacts with both histone H3 tail and DNA. Here, we describe biophysical and structural methods for characterization of the interactions between BRPF1PZP, H3 tail, DNA, and the intact nucleosome.For complete details on the use and execution of this protocol, please refer to Klein et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166721001866MicroscopyProtein BiochemistryProtein expression and purificationStructural BiologyX-ray CrystallographyNMR |
| spellingShingle | Brianna J. Klein Khan L. Cox Suk Min Jang Rohit K. Singh Jacques Côté Michael G. Poirier Tatiana G. Kutateladze Structural and biophysical characterization of the nucleosome-binding PZP domain STAR Protocols Microscopy Protein Biochemistry Protein expression and purification Structural Biology X-ray Crystallography NMR |
| title | Structural and biophysical characterization of the nucleosome-binding PZP domain |
| title_full | Structural and biophysical characterization of the nucleosome-binding PZP domain |
| title_fullStr | Structural and biophysical characterization of the nucleosome-binding PZP domain |
| title_full_unstemmed | Structural and biophysical characterization of the nucleosome-binding PZP domain |
| title_short | Structural and biophysical characterization of the nucleosome-binding PZP domain |
| title_sort | structural and biophysical characterization of the nucleosome binding pzp domain |
| topic | Microscopy Protein Biochemistry Protein expression and purification Structural Biology X-ray Crystallography NMR |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721001866 |
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