SARS-CoV-2 Circulation during the First Year of the Pandemic: A Seroprevalence Study from January to December 2020 in Tuscany, Italy
Italy was the second country affected by the SARS-CoV-2 pandemic; the virus spread mainly in Northern Italy with a subsequent diffusion to the center and southern part of the country. In this study, we aimed to assess the prevalence of antibodies against SARS-CoV-2 in the general population of the S...
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| Language: | English |
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MDPI AG
2022-06-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/14/7/1441 |
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| author | Serena Marchi Gianvito Lanave Michele Camero Francesca Dapporto Alessandro Manenti Linda Benincasa Angela Acciavatti Giulio Brogi Simonetta Viviani Emanuele Montomoli Claudia Maria Trombetta |
| author_facet | Serena Marchi Gianvito Lanave Michele Camero Francesca Dapporto Alessandro Manenti Linda Benincasa Angela Acciavatti Giulio Brogi Simonetta Viviani Emanuele Montomoli Claudia Maria Trombetta |
| author_sort | Serena Marchi |
| collection | DOAJ |
| description | Italy was the second country affected by the SARS-CoV-2 pandemic; the virus spread mainly in Northern Italy with a subsequent diffusion to the center and southern part of the country. In this study, we aimed to assess the prevalence of antibodies against SARS-CoV-2 in the general population of the Siena province in the Tuscany region (Central Italy) during 2020. A total of 2480 serum samples collected from January to December 2020 were tested for IgM and IgG antibodies against SARS-CoV-2 by a commercial ELISA. Positive and borderline samples were further tested for the presence of anti-receptor-binding domain (RBD) IgM and IgG antibodies by an in-house ELISA and by a micro-neutralization assay. Out of the 2480 samples tested by the commercial ELISA, 81 (3.3%) were found to be positive or borderline for IgG and 58 (2.3%) for IgM in a total of 133 samples (5.4%) found to be positive or borderline for at least one antibody class. When the commercial ELISA and in-house ELISA/micro-neutralization assay results were combined, 26 samples (1.0%) were positive for RBD IgG, 11 (0.4%) for RBD IgM, and 23 (0.9%) for a neutralizing antibody. An increase in seroprevalence was observed during the year 2020, especially from the end of summer, consistent with the routine epidemiological surveillance of COVID-19 cases. |
| first_indexed | 2024-03-09T05:43:25Z |
| format | Article |
| id | doaj.art-a0fec8e327fb43efb7934f5034653382 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T05:43:25Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-a0fec8e327fb43efb7934f50346533822023-12-03T12:23:04ZengMDPI AGViruses1999-49152022-06-01147144110.3390/v14071441SARS-CoV-2 Circulation during the First Year of the Pandemic: A Seroprevalence Study from January to December 2020 in Tuscany, ItalySerena Marchi0Gianvito Lanave1Michele Camero2Francesca Dapporto3Alessandro Manenti4Linda Benincasa5Angela Acciavatti6Giulio Brogi7Simonetta Viviani8Emanuele Montomoli9Claudia Maria Trombetta10Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, ItalyDepartment of Veterinary Medicine, University of Bari, 70010 Valenzano, ItalyDepartment of Veterinary Medicine, University of Bari, 70010 Valenzano, ItalyVisMederi Srl, 53100 Siena, ItalyVisMederi Srl, 53100 Siena, ItalyVisMederi Research Srl, 53100 Siena, ItalyNeoMedica Srl, 53100 Siena, ItalyNeoMedica Srl, 53100 Siena, ItalyDepartment of Molecular and Developmental Medicine, University of Siena, 53100 Siena, ItalyDepartment of Molecular and Developmental Medicine, University of Siena, 53100 Siena, ItalyDepartment of Molecular and Developmental Medicine, University of Siena, 53100 Siena, ItalyItaly was the second country affected by the SARS-CoV-2 pandemic; the virus spread mainly in Northern Italy with a subsequent diffusion to the center and southern part of the country. In this study, we aimed to assess the prevalence of antibodies against SARS-CoV-2 in the general population of the Siena province in the Tuscany region (Central Italy) during 2020. A total of 2480 serum samples collected from January to December 2020 were tested for IgM and IgG antibodies against SARS-CoV-2 by a commercial ELISA. Positive and borderline samples were further tested for the presence of anti-receptor-binding domain (RBD) IgM and IgG antibodies by an in-house ELISA and by a micro-neutralization assay. Out of the 2480 samples tested by the commercial ELISA, 81 (3.3%) were found to be positive or borderline for IgG and 58 (2.3%) for IgM in a total of 133 samples (5.4%) found to be positive or borderline for at least one antibody class. When the commercial ELISA and in-house ELISA/micro-neutralization assay results were combined, 26 samples (1.0%) were positive for RBD IgG, 11 (0.4%) for RBD IgM, and 23 (0.9%) for a neutralizing antibody. An increase in seroprevalence was observed during the year 2020, especially from the end of summer, consistent with the routine epidemiological surveillance of COVID-19 cases.https://www.mdpi.com/1999-4915/14/7/1441SARS-CoV-2Italyseroprevalence |
| spellingShingle | Serena Marchi Gianvito Lanave Michele Camero Francesca Dapporto Alessandro Manenti Linda Benincasa Angela Acciavatti Giulio Brogi Simonetta Viviani Emanuele Montomoli Claudia Maria Trombetta SARS-CoV-2 Circulation during the First Year of the Pandemic: A Seroprevalence Study from January to December 2020 in Tuscany, Italy Viruses SARS-CoV-2 Italy seroprevalence |
| title | SARS-CoV-2 Circulation during the First Year of the Pandemic: A Seroprevalence Study from January to December 2020 in Tuscany, Italy |
| title_full | SARS-CoV-2 Circulation during the First Year of the Pandemic: A Seroprevalence Study from January to December 2020 in Tuscany, Italy |
| title_fullStr | SARS-CoV-2 Circulation during the First Year of the Pandemic: A Seroprevalence Study from January to December 2020 in Tuscany, Italy |
| title_full_unstemmed | SARS-CoV-2 Circulation during the First Year of the Pandemic: A Seroprevalence Study from January to December 2020 in Tuscany, Italy |
| title_short | SARS-CoV-2 Circulation during the First Year of the Pandemic: A Seroprevalence Study from January to December 2020 in Tuscany, Italy |
| title_sort | sars cov 2 circulation during the first year of the pandemic a seroprevalence study from january to december 2020 in tuscany italy |
| topic | SARS-CoV-2 Italy seroprevalence |
| url | https://www.mdpi.com/1999-4915/14/7/1441 |
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