Diagnostic performance of an immunoassay based on urine exfoliated cell enrichment nanotechnology for upper tract urothelial carcinoma: a retrospective, monocentric study
Abstract Background Noninvasively urine-based diagnostic modalities for upper urinary tract urothelial carcinoma (UTUC) were still lacking. We evaluated the diagnostic value of our previously developed urine-based assay (UTC assay) in UTUC. Methods We retrospectively analyzed 90 patients with suspec...
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | BMC Urology |
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| Online Access: | https://doi.org/10.1186/s12894-022-01122-4 |
| _version_ | 1811186965155938304 |
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| author | Xin Wang Shiwei Zhang Lang Wu Baofu Feng Hongwei Shen Yuanyuan Gu Qun Zhang Feng Fang Rong Yang Hongqian Guo |
| author_facet | Xin Wang Shiwei Zhang Lang Wu Baofu Feng Hongwei Shen Yuanyuan Gu Qun Zhang Feng Fang Rong Yang Hongqian Guo |
| author_sort | Xin Wang |
| collection | DOAJ |
| description | Abstract Background Noninvasively urine-based diagnostic modalities for upper urinary tract urothelial carcinoma (UTUC) were still lacking. We evaluated the diagnostic value of our previously developed urine-based assay (UTC assay) in UTUC. Methods We retrospectively analyzed 90 patients with suspected UTUC and 40 donors without UTUC. Voided urine specimens were analyzed by UTC assay and fluorescence in situ hybridization (FISH). The performance of UTC assay and FISH was compared among the 60 histologically proven UTUC patients and the 40 donors with benign disease. Results Of the 60 UTUCs, there were 8 low-grade and 52 high-grade cases. Overall sensitivity for UTC assay and FISH were 85% and 73.3%, respectively (P = 0.116). Specificities for UTC assay and FISH were 92.5% and 95%, respectively (P = ns.). By grade, sensitivities of UTC assay and FISH were 87.5% vs. 37.5% for low-grade (P = 0.119), and 84.6% vs. 78.8% for high- grade UTUC (P = 0.446), respectively. By stage, UTC assay showed significantly higher sensitivity than FISH for detecting non-muscle-invasive UTUC, which were 88.5% vs. 61.5%, respectively (P = 0.025). Conclusion UTC assay has good performance for the non-invasive diagnosis of UTUC. UTC assay may improve the diagnosis and surveillance of low-grade or superficial UTUC. |
| first_indexed | 2024-04-11T13:54:00Z |
| format | Article |
| id | doaj.art-a0feea002ad04b6da0ebd8e8cdd31b96 |
| institution | Directory Open Access Journal |
| issn | 1471-2490 |
| language | English |
| last_indexed | 2024-04-11T13:54:00Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Urology |
| spelling | doaj.art-a0feea002ad04b6da0ebd8e8cdd31b962022-12-22T04:20:25ZengBMCBMC Urology1471-24902022-11-012211910.1186/s12894-022-01122-4Diagnostic performance of an immunoassay based on urine exfoliated cell enrichment nanotechnology for upper tract urothelial carcinoma: a retrospective, monocentric studyXin Wang0Shiwei Zhang1Lang Wu2Baofu Feng3Hongwei Shen4Yuanyuan Gu5Qun Zhang6Feng Fang7Rong Yang8Hongqian Guo9Department of Urology, Institute of Urology, Drum Tower Hospital, Medical School of Nanjing University, Nanjing UniversityDepartment of Urology, Institute of Urology, Drum Tower Hospital, Medical School of Nanjing University, Nanjing UniversityPerMed Biomedicine InstituteDepartment of Urology, Institute of Urology, Drum Tower Hospital, Medical School of Nanjing University, Nanjing UniversityDepartment of Urology, Institute of Urology, Drum Tower Hospital, Medical School of Nanjing University, Nanjing UniversityPerMed Biomedicine InstitutePerMed Biomedicine InstituteDepartment of Pharmacology, Nanjing Medical UniversityDepartment of Urology, Institute of Urology, Drum Tower Hospital, Medical School of Nanjing University, Nanjing UniversityDepartment of Urology, Institute of Urology, Drum Tower Hospital, Medical School of Nanjing University, Nanjing UniversityAbstract Background Noninvasively urine-based diagnostic modalities for upper urinary tract urothelial carcinoma (UTUC) were still lacking. We evaluated the diagnostic value of our previously developed urine-based assay (UTC assay) in UTUC. Methods We retrospectively analyzed 90 patients with suspected UTUC and 40 donors without UTUC. Voided urine specimens were analyzed by UTC assay and fluorescence in situ hybridization (FISH). The performance of UTC assay and FISH was compared among the 60 histologically proven UTUC patients and the 40 donors with benign disease. Results Of the 60 UTUCs, there were 8 low-grade and 52 high-grade cases. Overall sensitivity for UTC assay and FISH were 85% and 73.3%, respectively (P = 0.116). Specificities for UTC assay and FISH were 92.5% and 95%, respectively (P = ns.). By grade, sensitivities of UTC assay and FISH were 87.5% vs. 37.5% for low-grade (P = 0.119), and 84.6% vs. 78.8% for high- grade UTUC (P = 0.446), respectively. By stage, UTC assay showed significantly higher sensitivity than FISH for detecting non-muscle-invasive UTUC, which were 88.5% vs. 61.5%, respectively (P = 0.025). Conclusion UTC assay has good performance for the non-invasive diagnosis of UTUC. UTC assay may improve the diagnosis and surveillance of low-grade or superficial UTUC.https://doi.org/10.1186/s12894-022-01122-4Urothelial carcinomaUpper urinary tractUrineImmunocytologyFISH |
| spellingShingle | Xin Wang Shiwei Zhang Lang Wu Baofu Feng Hongwei Shen Yuanyuan Gu Qun Zhang Feng Fang Rong Yang Hongqian Guo Diagnostic performance of an immunoassay based on urine exfoliated cell enrichment nanotechnology for upper tract urothelial carcinoma: a retrospective, monocentric study BMC Urology Urothelial carcinoma Upper urinary tract Urine Immunocytology FISH |
| title | Diagnostic performance of an immunoassay based on urine exfoliated cell enrichment nanotechnology for upper tract urothelial carcinoma: a retrospective, monocentric study |
| title_full | Diagnostic performance of an immunoassay based on urine exfoliated cell enrichment nanotechnology for upper tract urothelial carcinoma: a retrospective, monocentric study |
| title_fullStr | Diagnostic performance of an immunoassay based on urine exfoliated cell enrichment nanotechnology for upper tract urothelial carcinoma: a retrospective, monocentric study |
| title_full_unstemmed | Diagnostic performance of an immunoassay based on urine exfoliated cell enrichment nanotechnology for upper tract urothelial carcinoma: a retrospective, monocentric study |
| title_short | Diagnostic performance of an immunoassay based on urine exfoliated cell enrichment nanotechnology for upper tract urothelial carcinoma: a retrospective, monocentric study |
| title_sort | diagnostic performance of an immunoassay based on urine exfoliated cell enrichment nanotechnology for upper tract urothelial carcinoma a retrospective monocentric study |
| topic | Urothelial carcinoma Upper urinary tract Urine Immunocytology FISH |
| url | https://doi.org/10.1186/s12894-022-01122-4 |
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