Leukemia microvesicles affect healthy hematopoietic stem cells
Microvesicles are released by different cell types and shuttle mRNAs and microRNAs which have the possibility to transfer genetic information to a target cell and alter its function. Acute myeloid leukemia is a malignant disorder, and leukemic cells occupy all the bone marrow microenvironment. In th...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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IOS Press
2017-02-01
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| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317692234 |
| _version_ | 1818865947200454656 |
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| author | Farnaz Razmkhah Masoud Soleimani Davood Mehrabani Mohammad Hossein Karimi Sedigheh Amini Kafi-abad Mani Ramzi Mahdiyar Iravani Saadi Javad Kakoui |
| author_facet | Farnaz Razmkhah Masoud Soleimani Davood Mehrabani Mohammad Hossein Karimi Sedigheh Amini Kafi-abad Mani Ramzi Mahdiyar Iravani Saadi Javad Kakoui |
| author_sort | Farnaz Razmkhah |
| collection | DOAJ |
| description | Microvesicles are released by different cell types and shuttle mRNAs and microRNAs which have the possibility to transfer genetic information to a target cell and alter its function. Acute myeloid leukemia is a malignant disorder, and leukemic cells occupy all the bone marrow microenvironment. In this study, we investigate the effect of leukemia microvesicles on healthy umbilical cord blood hematopoietic stem cells to find evidence of cell information transferring. Leukemia microvesicles were isolated from acute myeloid leukemia patients and were co-incubated with healthy hematopoietic stem cells. After 7 days, cell count, hematopoietic stem cell–specific cluster of differentiation (CD) markers, colony-forming unit assay, and some microRNA gene expressions were assessed. Data showed a higher number of hematopoietic stem cells after being treated with leukemia microvesicles compared with control (treated with no microvesicles) and normal (treated with normal microvesicles) groups. Also, increased levels of microRNA-21 and microRNA-29a genes were observed in this group, while colony-forming ability was still maintained and high ranges of CD34 + , CD34 + CD38 − , CD90 + , and CD117 + phenotypes were observed as stemness signs. Our results suggest that leukemia microvesicles are able to induce some effects on healthy hematopoietic stem cells such as promoting cell survival and some microRNAs deregulation, while stemness is maintained. |
| first_indexed | 2024-12-19T10:55:38Z |
| format | Article |
| id | doaj.art-a0ff5a05b0dd481e86065b297ea08080 |
| institution | Directory Open Access Journal |
| issn | 1423-0380 |
| language | English |
| last_indexed | 2024-12-19T10:55:38Z |
| publishDate | 2017-02-01 |
| publisher | IOS Press |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj.art-a0ff5a05b0dd481e86065b297ea080802022-12-21T20:24:49ZengIOS PressTumor Biology1423-03802017-02-013910.1177/1010428317692234Leukemia microvesicles affect healthy hematopoietic stem cellsFarnaz Razmkhah0Masoud Soleimani1Davood Mehrabani2Mohammad Hossein Karimi3Sedigheh Amini Kafi-abad4Mani Ramzi5Mahdiyar Iravani Saadi6Javad Kakoui7Department of Hematology, Faculty of Medicine, Tarbiat Modares University, Tehran, IranDepartment of Hematology, Faculty of Medicine, Tarbiat Modares University, Tehran, IranStem cell technology research center, Shiraz University of Medical Sciences, Shiraz, IranTransplant Research Center, Shiraz University of Medical Sciences, Shiraz, IranBlood Transfusion Research Center, Department of Pathology, High Institute for Research and Education in Transfusion Medicine, Tehran, IranHematology research center, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Hematology-Oncology and Stem Cell Transplantation, Shiraz University of Medical Sciences, Shiraz, IranAtieh Hospital, Tehran, IranMicrovesicles are released by different cell types and shuttle mRNAs and microRNAs which have the possibility to transfer genetic information to a target cell and alter its function. Acute myeloid leukemia is a malignant disorder, and leukemic cells occupy all the bone marrow microenvironment. In this study, we investigate the effect of leukemia microvesicles on healthy umbilical cord blood hematopoietic stem cells to find evidence of cell information transferring. Leukemia microvesicles were isolated from acute myeloid leukemia patients and were co-incubated with healthy hematopoietic stem cells. After 7 days, cell count, hematopoietic stem cell–specific cluster of differentiation (CD) markers, colony-forming unit assay, and some microRNA gene expressions were assessed. Data showed a higher number of hematopoietic stem cells after being treated with leukemia microvesicles compared with control (treated with no microvesicles) and normal (treated with normal microvesicles) groups. Also, increased levels of microRNA-21 and microRNA-29a genes were observed in this group, while colony-forming ability was still maintained and high ranges of CD34 + , CD34 + CD38 − , CD90 + , and CD117 + phenotypes were observed as stemness signs. Our results suggest that leukemia microvesicles are able to induce some effects on healthy hematopoietic stem cells such as promoting cell survival and some microRNAs deregulation, while stemness is maintained.https://doi.org/10.1177/1010428317692234 |
| spellingShingle | Farnaz Razmkhah Masoud Soleimani Davood Mehrabani Mohammad Hossein Karimi Sedigheh Amini Kafi-abad Mani Ramzi Mahdiyar Iravani Saadi Javad Kakoui Leukemia microvesicles affect healthy hematopoietic stem cells Tumor Biology |
| title | Leukemia microvesicles affect healthy hematopoietic stem cells |
| title_full | Leukemia microvesicles affect healthy hematopoietic stem cells |
| title_fullStr | Leukemia microvesicles affect healthy hematopoietic stem cells |
| title_full_unstemmed | Leukemia microvesicles affect healthy hematopoietic stem cells |
| title_short | Leukemia microvesicles affect healthy hematopoietic stem cells |
| title_sort | leukemia microvesicles affect healthy hematopoietic stem cells |
| url | https://doi.org/10.1177/1010428317692234 |
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