Nanoscale correlative X-ray spectroscopy and ptychography of carious dental enamel
This study reports the characterisation of human dental enamel caries using synchrotron nanoscale correlative ptychography and spectroscopic mapping in combination with scanning electron microscopy. A lamella ̴2.4 µm thick was extracted from a carious enamel region of a tooth using focused ion beam-...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Materials & Design |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127522008942 |
| _version_ | 1811292065577828352 |
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| author | Cyril Besnard Ali Marie Sisini Sasidharan Petr Buček Jessica M. Walker Julia E. Parker Thomas E.J. Moxham Benedikt Daurer Burkhard Kaulich Majid Kazemian Richard M. Shelton Gabriel Landini Alexander M. Korsunsky |
| author_facet | Cyril Besnard Ali Marie Sisini Sasidharan Petr Buček Jessica M. Walker Julia E. Parker Thomas E.J. Moxham Benedikt Daurer Burkhard Kaulich Majid Kazemian Richard M. Shelton Gabriel Landini Alexander M. Korsunsky |
| author_sort | Cyril Besnard |
| collection | DOAJ |
| description | This study reports the characterisation of human dental enamel caries using synchrotron nanoscale correlative ptychography and spectroscopic mapping in combination with scanning electron microscopy. A lamella ̴2.4 µm thick was extracted from a carious enamel region of a tooth using focused ion beam-scanning electron microscopy and transferred to two synchrotron beamlines to perform hard X-ray nano-fluorescence spectroscopy simultaneously with differential phase contrast mapping at a beam size of 55 × 45 nm. Soft X-ray ptychography data was then reconstructed with a pixel size of 8 nm. The two dimensional variation in chemistry and structure of carious enamel was revealed at the nanoscale, namely, the organisation of hydroxyapatite nano-crystals within enamel rods was imaged together with the inter-rod region. Correlative use of electron and X-ray scanning microscopies for the same sample allowed visualisation of the connection between structure and composition as presented in a compound image where colour indicates the relative calcium concentration in the sample, as indicated by the calcium Kα fluorescence intensity and grey scale shows the nanostructure. This highlights the importance of advanced correlative imaging to investigate the complex structure-composition relationships in nanomaterials of natural or artificial origin. |
| first_indexed | 2024-04-13T04:38:59Z |
| format | Article |
| id | doaj.art-a1032d9a078c44cdb8613124c0d9a6e9 |
| institution | Directory Open Access Journal |
| issn | 0264-1275 |
| language | English |
| last_indexed | 2024-04-13T04:38:59Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj.art-a1032d9a078c44cdb8613124c0d9a6e92022-12-22T03:02:04ZengElsevierMaterials & Design0264-12752022-12-01224111272Nanoscale correlative X-ray spectroscopy and ptychography of carious dental enamelCyril Besnard0Ali Marie1Sisini Sasidharan2Petr Buček3Jessica M. Walker4Julia E. Parker5Thomas E.J. Moxham6Benedikt Daurer7Burkhard Kaulich8Majid Kazemian9Richard M. Shelton10Gabriel Landini11Alexander M. Korsunsky12MBLEM, Department of Engineering Science, University of Oxford, Parks Road, Oxford, Oxfordshire OX1 3PJ, UK; Corresponding authors.MBLEM, Department of Engineering Science, University of Oxford, Parks Road, Oxford, Oxfordshire OX1 3PJ, UKMBLEM, Department of Engineering Science, University of Oxford, Parks Road, Oxford, Oxfordshire OX1 3PJ, UKTESCAN-UK Ltd., Wellbrook Court, Girton, Cambridge CB3 0NA, UKDiamond Light Source Ltd., Didcot, Oxfordshire OX11 0DE, UKDiamond Light Source Ltd., Didcot, Oxfordshire OX11 0DE, UKMBLEM, Department of Engineering Science, University of Oxford, Parks Road, Oxford, Oxfordshire OX1 3PJ, UK; Diamond Light Source Ltd., Didcot, Oxfordshire OX11 0DE, UKDiamond Light Source Ltd., Didcot, Oxfordshire OX11 0DE, UKDiamond Light Source Ltd., Didcot, Oxfordshire OX11 0DE, UKDiamond Light Source Ltd., Didcot, Oxfordshire OX11 0DE, UKSchool of Dentistry, University of Birmingham, 5 Mill Pool Way, Edgbaston, Birmingham, West Midlands B5 7EG, UKSchool of Dentistry, University of Birmingham, 5 Mill Pool Way, Edgbaston, Birmingham, West Midlands B5 7EG, UKMBLEM, Department of Engineering Science, University of Oxford, Parks Road, Oxford, Oxfordshire OX1 3PJ, UK; Corresponding authors.This study reports the characterisation of human dental enamel caries using synchrotron nanoscale correlative ptychography and spectroscopic mapping in combination with scanning electron microscopy. A lamella ̴2.4 µm thick was extracted from a carious enamel region of a tooth using focused ion beam-scanning electron microscopy and transferred to two synchrotron beamlines to perform hard X-ray nano-fluorescence spectroscopy simultaneously with differential phase contrast mapping at a beam size of 55 × 45 nm. Soft X-ray ptychography data was then reconstructed with a pixel size of 8 nm. The two dimensional variation in chemistry and structure of carious enamel was revealed at the nanoscale, namely, the organisation of hydroxyapatite nano-crystals within enamel rods was imaged together with the inter-rod region. Correlative use of electron and X-ray scanning microscopies for the same sample allowed visualisation of the connection between structure and composition as presented in a compound image where colour indicates the relative calcium concentration in the sample, as indicated by the calcium Kα fluorescence intensity and grey scale shows the nanostructure. This highlights the importance of advanced correlative imaging to investigate the complex structure-composition relationships in nanomaterials of natural or artificial origin.http://www.sciencedirect.com/science/article/pii/S0264127522008942Human enamel cariesSynchrotron correlative nanoanalysisX-ray fluorescence spectroscopyX-ray differential phase contrastX-ray ptychographyFIB-SEM |
| spellingShingle | Cyril Besnard Ali Marie Sisini Sasidharan Petr Buček Jessica M. Walker Julia E. Parker Thomas E.J. Moxham Benedikt Daurer Burkhard Kaulich Majid Kazemian Richard M. Shelton Gabriel Landini Alexander M. Korsunsky Nanoscale correlative X-ray spectroscopy and ptychography of carious dental enamel Materials & Design Human enamel caries Synchrotron correlative nanoanalysis X-ray fluorescence spectroscopy X-ray differential phase contrast X-ray ptychography FIB-SEM |
| title | Nanoscale correlative X-ray spectroscopy and ptychography of carious dental enamel |
| title_full | Nanoscale correlative X-ray spectroscopy and ptychography of carious dental enamel |
| title_fullStr | Nanoscale correlative X-ray spectroscopy and ptychography of carious dental enamel |
| title_full_unstemmed | Nanoscale correlative X-ray spectroscopy and ptychography of carious dental enamel |
| title_short | Nanoscale correlative X-ray spectroscopy and ptychography of carious dental enamel |
| title_sort | nanoscale correlative x ray spectroscopy and ptychography of carious dental enamel |
| topic | Human enamel caries Synchrotron correlative nanoanalysis X-ray fluorescence spectroscopy X-ray differential phase contrast X-ray ptychography FIB-SEM |
| url | http://www.sciencedirect.com/science/article/pii/S0264127522008942 |
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