Serum Metabolomics Analysis for Biomarkers of <i>Lactobacillus plantarum</i> FRT4 in High-Fat Diet-Induced Obese Mice
<i>Lactobacillus plantarum</i> is considered a potential probiotic supplementation for treating obesity. However, the underlying molecular mechanism is poorly understood. Our previous study displayed that <i>L. plantarum</i> FRT4 alleviated obesity in mice fed a high-fat diet...
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MDPI AG
2022-01-01
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| author | Hongying Cai Zhiguo Wen Xin Xu Jiaxin Wang Xuan Li Kun Meng Peilong Yang |
| author_facet | Hongying Cai Zhiguo Wen Xin Xu Jiaxin Wang Xuan Li Kun Meng Peilong Yang |
| author_sort | Hongying Cai |
| collection | DOAJ |
| description | <i>Lactobacillus plantarum</i> is considered a potential probiotic supplementation for treating obesity. However, the underlying molecular mechanism is poorly understood. Our previous study displayed that <i>L. plantarum</i> FRT4 alleviated obesity in mice fed a high-fat diet (HFD) through ameliorating the HFD-induced gut microbiota dysbiosis. To explore the roles of FRT4 in obesity prevention, in this study, we investigated changes in serum metabolomic phenotype by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS) and analyzed the pathway of HFD-fed Kunming female mice orally administered with FRT4 for eight weeks. Using orthogonal partial least squares discriminant analysis (OPLS-DA), metabolite patterns with significant changes were observed. 55 metabolites including phosphatidylcholine, lysophophatidylcholine, sphingomyelin, serotonin, indole-3-methyl aceta, indole-3-carbinol, indole-5,6-quino, 11,12-DHET, prostaglandin B2, leukotriene B4, and 3-hydroxybenzoic acid were identified as potential biomarkers associated with obesity, which were mainly involving in glycerophospholipid metabolism, tryptophan metabolism, and arachidonic acid metabolism. Perturbations of 14 biomarkers could be regulated by FRT4 intervention. These metabolites may serve as valuable biomarkers to understand the mechanisms by which intake of diets containing FRT4 contributes to the treatment or prevention of obesity. Thus, FRT4 can be a promising dietary supplement for the prevention of HFD-induced obesity. |
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| language | English |
| last_indexed | 2024-03-10T01:29:54Z |
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| spelling | doaj.art-a104d2eb6ea5459d92a4160630ed95ae2023-11-23T13:45:04ZengMDPI AGFoods2304-81582022-01-0111218410.3390/foods11020184Serum Metabolomics Analysis for Biomarkers of <i>Lactobacillus plantarum</i> FRT4 in High-Fat Diet-Induced Obese MiceHongying Cai0Zhiguo Wen1Xin Xu2Jiaxin Wang3Xuan Li4Kun Meng5Peilong Yang6Key Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKey Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKey Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKey Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKey Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKey Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaKey Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing 100081, China<i>Lactobacillus plantarum</i> is considered a potential probiotic supplementation for treating obesity. However, the underlying molecular mechanism is poorly understood. Our previous study displayed that <i>L. plantarum</i> FRT4 alleviated obesity in mice fed a high-fat diet (HFD) through ameliorating the HFD-induced gut microbiota dysbiosis. To explore the roles of FRT4 in obesity prevention, in this study, we investigated changes in serum metabolomic phenotype by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS) and analyzed the pathway of HFD-fed Kunming female mice orally administered with FRT4 for eight weeks. Using orthogonal partial least squares discriminant analysis (OPLS-DA), metabolite patterns with significant changes were observed. 55 metabolites including phosphatidylcholine, lysophophatidylcholine, sphingomyelin, serotonin, indole-3-methyl aceta, indole-3-carbinol, indole-5,6-quino, 11,12-DHET, prostaglandin B2, leukotriene B4, and 3-hydroxybenzoic acid were identified as potential biomarkers associated with obesity, which were mainly involving in glycerophospholipid metabolism, tryptophan metabolism, and arachidonic acid metabolism. Perturbations of 14 biomarkers could be regulated by FRT4 intervention. These metabolites may serve as valuable biomarkers to understand the mechanisms by which intake of diets containing FRT4 contributes to the treatment or prevention of obesity. Thus, FRT4 can be a promising dietary supplement for the prevention of HFD-induced obesity.https://www.mdpi.com/2304-8158/11/2/184obesity<i>Lactobacillus plantarum</i> FRT4metabolomicsbiomarkerUHPLC-QTOF/MS |
| spellingShingle | Hongying Cai Zhiguo Wen Xin Xu Jiaxin Wang Xuan Li Kun Meng Peilong Yang Serum Metabolomics Analysis for Biomarkers of <i>Lactobacillus plantarum</i> FRT4 in High-Fat Diet-Induced Obese Mice Foods obesity <i>Lactobacillus plantarum</i> FRT4 metabolomics biomarker UHPLC-QTOF/MS |
| title | Serum Metabolomics Analysis for Biomarkers of <i>Lactobacillus plantarum</i> FRT4 in High-Fat Diet-Induced Obese Mice |
| title_full | Serum Metabolomics Analysis for Biomarkers of <i>Lactobacillus plantarum</i> FRT4 in High-Fat Diet-Induced Obese Mice |
| title_fullStr | Serum Metabolomics Analysis for Biomarkers of <i>Lactobacillus plantarum</i> FRT4 in High-Fat Diet-Induced Obese Mice |
| title_full_unstemmed | Serum Metabolomics Analysis for Biomarkers of <i>Lactobacillus plantarum</i> FRT4 in High-Fat Diet-Induced Obese Mice |
| title_short | Serum Metabolomics Analysis for Biomarkers of <i>Lactobacillus plantarum</i> FRT4 in High-Fat Diet-Induced Obese Mice |
| title_sort | serum metabolomics analysis for biomarkers of i lactobacillus plantarum i frt4 in high fat diet induced obese mice |
| topic | obesity <i>Lactobacillus plantarum</i> FRT4 metabolomics biomarker UHPLC-QTOF/MS |
| url | https://www.mdpi.com/2304-8158/11/2/184 |
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