The oral cavity and intestinal microbiome in children with functional constipation
Abstract Constipation is a widespread problem in paediatric practice, affecting almost 30% of children. One of the key causal factors of constipation may be disturbances in the homeostasis of the gastrointestinal microbiome. The aim of the study was to determine whether the oral and fecal microbiome...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-58642-2 |
| _version_ | 1797209361331781632 |
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| author | Monika Kwiatkowska Marcin Gołębiewski Marcin Sikora Ewa Łoś Rycharska Aneta Krogulska |
| author_facet | Monika Kwiatkowska Marcin Gołębiewski Marcin Sikora Ewa Łoś Rycharska Aneta Krogulska |
| author_sort | Monika Kwiatkowska |
| collection | DOAJ |
| description | Abstract Constipation is a widespread problem in paediatric practice, affecting almost 30% of children. One of the key causal factors of constipation may be disturbances in the homeostasis of the gastrointestinal microbiome. The aim of the study was to determine whether the oral and fecal microbiomes differ between children with and without constipation. A total of 91 children over three years of age were included in the study. Of these, 57 were qualified to a group with constipation, and 34 to a group without. The saliva and stool microbiomes were evaluated using 16S rRNA gene amplicon sequencing. Functional constipation was associated with characteristic bacterial taxa in the fecal microbiota. Statistically significant differences were found at the family level: Burkholderiaceae (q = 0.047), Christensenellaceae (q = 0.047), Chlostridiaceae (q = 0.047) were significantly less abundant in the constipation group, while the Tannerellaceae (q = 0.007) were more abundant. At the genus level, the significant differences were observed for rare genera, including Christensenellaceae r-7 (q = 2.88 × 10−2), Fusicatenibacter (q = 2.88 × 10−2), Parabacteroides (q = 1.63 × 10−2), Romboutsia (q = 3.19 × 10−2) and Subdoligranulum (q = 1.17 × 10−2). All of them were less abundant in children with constipation. With the exception of significant taxonomic changes affecting only feces, no differences were found in the alpha and beta diversity of feces and saliva. Children with functional constipation demonstrated significant differences in the abundance of specific bacteria in the stool microbiome compared to healthy children. It is possible that the rare genera identified in our study which were less abundant in the constipated patients (Christensellaceae r-7, Fusicatenibacter, Parabacteroides, Romboutsia and Subdoligranulum) may play a role in protection against constipation. No significant differences were observed between the two groups with regard to the saliva microbiome. |
| first_indexed | 2024-04-24T09:53:29Z |
| format | Article |
| id | doaj.art-a106049c402641f19a9f5fb079ef78e8 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-24T09:53:29Z |
| publishDate | 2024-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a106049c402641f19a9f5fb079ef78e82024-04-14T11:15:31ZengNature PortfolioScientific Reports2045-23222024-04-0114111310.1038/s41598-024-58642-2The oral cavity and intestinal microbiome in children with functional constipationMonika Kwiatkowska0Marcin Gołębiewski1Marcin Sikora2Ewa Łoś Rycharska3Aneta Krogulska4Department of Paediatrics, Allergology and Gastroenterology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus UniversityDepartment of Plant Physiology and Biotechnology, Nicolaus Copernicus UniversityInterdisciplinary Centre of Modern Technologies, Nicolaus Copernicus UniversityDepartment of Paediatrics, Allergology and Gastroenterology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus UniversityDepartment of Paediatrics, Allergology and Gastroenterology, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus UniversityAbstract Constipation is a widespread problem in paediatric practice, affecting almost 30% of children. One of the key causal factors of constipation may be disturbances in the homeostasis of the gastrointestinal microbiome. The aim of the study was to determine whether the oral and fecal microbiomes differ between children with and without constipation. A total of 91 children over three years of age were included in the study. Of these, 57 were qualified to a group with constipation, and 34 to a group without. The saliva and stool microbiomes were evaluated using 16S rRNA gene amplicon sequencing. Functional constipation was associated with characteristic bacterial taxa in the fecal microbiota. Statistically significant differences were found at the family level: Burkholderiaceae (q = 0.047), Christensenellaceae (q = 0.047), Chlostridiaceae (q = 0.047) were significantly less abundant in the constipation group, while the Tannerellaceae (q = 0.007) were more abundant. At the genus level, the significant differences were observed for rare genera, including Christensenellaceae r-7 (q = 2.88 × 10−2), Fusicatenibacter (q = 2.88 × 10−2), Parabacteroides (q = 1.63 × 10−2), Romboutsia (q = 3.19 × 10−2) and Subdoligranulum (q = 1.17 × 10−2). All of them were less abundant in children with constipation. With the exception of significant taxonomic changes affecting only feces, no differences were found in the alpha and beta diversity of feces and saliva. Children with functional constipation demonstrated significant differences in the abundance of specific bacteria in the stool microbiome compared to healthy children. It is possible that the rare genera identified in our study which were less abundant in the constipated patients (Christensellaceae r-7, Fusicatenibacter, Parabacteroides, Romboutsia and Subdoligranulum) may play a role in protection against constipation. No significant differences were observed between the two groups with regard to the saliva microbiome.https://doi.org/10.1038/s41598-024-58642-2 |
| spellingShingle | Monika Kwiatkowska Marcin Gołębiewski Marcin Sikora Ewa Łoś Rycharska Aneta Krogulska The oral cavity and intestinal microbiome in children with functional constipation Scientific Reports |
| title | The oral cavity and intestinal microbiome in children with functional constipation |
| title_full | The oral cavity and intestinal microbiome in children with functional constipation |
| title_fullStr | The oral cavity and intestinal microbiome in children with functional constipation |
| title_full_unstemmed | The oral cavity and intestinal microbiome in children with functional constipation |
| title_short | The oral cavity and intestinal microbiome in children with functional constipation |
| title_sort | oral cavity and intestinal microbiome in children with functional constipation |
| url | https://doi.org/10.1038/s41598-024-58642-2 |
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