Lomasomes and Other Fungal Plasma Membrane Macroinvaginations Have a Tubular and Lamellar Genesis
The plasma membrane of filamentous fungi forms large-sized invaginations, which are either tubes or parietal vesicles. Vesicular macroinvaginations at the ultrastructural level correspond to classical lomasomes. There is an assumption that vesicular macroinvaginations/lomasomes may be involved in ma...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/12/1316 |
| _version_ | 1797456877418708992 |
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| author | Igor S. Mazheika Nadezhda V. Psurtseva Olga V. Kamzolkina |
| author_facet | Igor S. Mazheika Nadezhda V. Psurtseva Olga V. Kamzolkina |
| author_sort | Igor S. Mazheika |
| collection | DOAJ |
| description | The plasma membrane of filamentous fungi forms large-sized invaginations, which are either tubes or parietal vesicles. Vesicular macroinvaginations at the ultrastructural level correspond to classical lomasomes. There is an assumption that vesicular macroinvaginations/lomasomes may be involved in macrovesicular endocytosis. The original aim of this study was to test for the presence of macroendocytosis in xylotrophic basidiomycetes using time-lapse and Z-stacks fluorescent microscopic technologies. However, the results were unexpected since most of the membrane structures labeled by the endocytic tracer (FM4-64 analog) are various types of plasma membrane macroinvaginations and not any endomembranes. All of these macroinvaginations have a tubular or lamellar genesis. Moreover, under specific conditions of a microscopic preparation, the diameter of the tubes forming the macroinvaginations increases with the time of the sample observation. In addition, the morphology and successive formation of the macroinvaginations mimic the endocytic pathway; these invaginations can easily be mistaken for endocytic vesicles, endosomes, and vacuole-lysosomes. The paper analyzes the various macroinvagination types, suggests their biological functions, and discusses some features of fungal endocytosis. This study is a next step toward understanding complex fungal physiology and is a presentation of a new intracellular tubular system in wood-decaying fungi. |
| first_indexed | 2024-03-09T16:14:07Z |
| format | Article |
| id | doaj.art-a107ad6f3a2e43f8b46ce9ab40164793 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T16:14:07Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-a107ad6f3a2e43f8b46ce9ab401647932023-11-24T16:00:24ZengMDPI AGJournal of Fungi2309-608X2022-12-01812131610.3390/jof8121316Lomasomes and Other Fungal Plasma Membrane Macroinvaginations Have a Tubular and Lamellar GenesisIgor S. Mazheika0Nadezhda V. Psurtseva1Olga V. Kamzolkina2Department of mycology and algology, Lomonosov Moscow State University, 119991 Moscow, RussiaKomarov Botanical Institute, Russian Academy of Sciences, 197376 St. Petersburg, RussiaDepartment of mycology and algology, Lomonosov Moscow State University, 119991 Moscow, RussiaThe plasma membrane of filamentous fungi forms large-sized invaginations, which are either tubes or parietal vesicles. Vesicular macroinvaginations at the ultrastructural level correspond to classical lomasomes. There is an assumption that vesicular macroinvaginations/lomasomes may be involved in macrovesicular endocytosis. The original aim of this study was to test for the presence of macroendocytosis in xylotrophic basidiomycetes using time-lapse and Z-stacks fluorescent microscopic technologies. However, the results were unexpected since most of the membrane structures labeled by the endocytic tracer (FM4-64 analog) are various types of plasma membrane macroinvaginations and not any endomembranes. All of these macroinvaginations have a tubular or lamellar genesis. Moreover, under specific conditions of a microscopic preparation, the diameter of the tubes forming the macroinvaginations increases with the time of the sample observation. In addition, the morphology and successive formation of the macroinvaginations mimic the endocytic pathway; these invaginations can easily be mistaken for endocytic vesicles, endosomes, and vacuole-lysosomes. The paper analyzes the various macroinvagination types, suggests their biological functions, and discusses some features of fungal endocytosis. This study is a next step toward understanding complex fungal physiology and is a presentation of a new intracellular tubular system in wood-decaying fungi.https://www.mdpi.com/2309-608X/8/12/1316wood-decaying fungiendocytosisZ-stackstime-lapse video |
| spellingShingle | Igor S. Mazheika Nadezhda V. Psurtseva Olga V. Kamzolkina Lomasomes and Other Fungal Plasma Membrane Macroinvaginations Have a Tubular and Lamellar Genesis Journal of Fungi wood-decaying fungi endocytosis Z-stacks time-lapse video |
| title | Lomasomes and Other Fungal Plasma Membrane Macroinvaginations Have a Tubular and Lamellar Genesis |
| title_full | Lomasomes and Other Fungal Plasma Membrane Macroinvaginations Have a Tubular and Lamellar Genesis |
| title_fullStr | Lomasomes and Other Fungal Plasma Membrane Macroinvaginations Have a Tubular and Lamellar Genesis |
| title_full_unstemmed | Lomasomes and Other Fungal Plasma Membrane Macroinvaginations Have a Tubular and Lamellar Genesis |
| title_short | Lomasomes and Other Fungal Plasma Membrane Macroinvaginations Have a Tubular and Lamellar Genesis |
| title_sort | lomasomes and other fungal plasma membrane macroinvaginations have a tubular and lamellar genesis |
| topic | wood-decaying fungi endocytosis Z-stacks time-lapse video |
| url | https://www.mdpi.com/2309-608X/8/12/1316 |
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