Natural Hemp-Ginger Extract and Its Biological and Therapeutic Efficacy
The prevention and treatment of skin diseases remains a major challenge in medicine. The search for natural active ingredients that can be used to prevent the development of the disease and complement treatment is on the rise. Natural extracts of ginger and hemp offer a wide range of bioactive compo...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/22/7694 |
| _version_ | 1797464438631038976 |
|---|---|
| author | Taja Žitek Dragana Bjelić Petra Kotnik Andrej Golle Staša Jurgec Uroš Potočnik Željko Knez Matjaž Finšgar Ivan Krajnc Igor Krajnc Maša Knez Marevci |
| author_facet | Taja Žitek Dragana Bjelić Petra Kotnik Andrej Golle Staša Jurgec Uroš Potočnik Željko Knez Matjaž Finšgar Ivan Krajnc Igor Krajnc Maša Knez Marevci |
| author_sort | Taja Žitek |
| collection | DOAJ |
| description | The prevention and treatment of skin diseases remains a major challenge in medicine. The search for natural active ingredients that can be used to prevent the development of the disease and complement treatment is on the rise. Natural extracts of ginger and hemp offer a wide range of bioactive compounds with potential health benefits. This study evaluates the effectiveness of hemp and ginger extract as a supportive treatment for skin diseases. It reports a synergistic effect of hemp and ginger extract. The contents of cannabinoids and components of ginger are determined, with the highest being CBD (587.17 ± 8.32 µg/g) and 6-gingerol (60.07 ± 0.40 µg/g). The minimum inhibitory concentration for <i>Staphylococcus aureus</i> (156.5 µg/mL), <i>Escherichia coli</i> (625.2 µg/mL) and <i>Candida albicans</i> (78.3 µg/mL) was also analyzed. Analysis of WM-266-4 cells revealed the greatest decrease in metabolic activity in cells exposed to the extract at a concentration of 1.00 µg/mL. Regarding the expression of genes associated with cellular processes, melanoma aggressiveness, resistance and cell survival, a significant difference was found in the expression of <i>ABCB5</i>, <i>CAV1</i> and <i>S100A9</i> compared with the control (cells not exposed to the extract). |
| first_indexed | 2024-03-09T18:08:11Z |
| format | Article |
| id | doaj.art-a1081068912b411fb7fb22445f2bb681 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T18:08:11Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a1081068912b411fb7fb22445f2bb6812023-11-24T09:19:33ZengMDPI AGMolecules1420-30492022-11-012722769410.3390/molecules27227694Natural Hemp-Ginger Extract and Its Biological and Therapeutic EfficacyTaja Žitek0Dragana Bjelić1Petra Kotnik2Andrej Golle3Staša Jurgec4Uroš Potočnik5Željko Knez6Matjaž Finšgar7Ivan Krajnc8Igor Krajnc9Maša Knez Marevci10Laboratory for Separation Processes and Product Design, Faculty of Chemistry and Chemical Engineering, University of Maribor, Smetanova ul. 17, 2000 Maribor, SloveniaLaboratory for Analytical Chemistry and Industrial Analysis, Faculty of Chemistry and Chemical Engineering, University of Maribor, Smetanova ul. 17, 2000 Maribor, SloveniaLaboratory for Separation Processes and Product Design, Faculty of Chemistry and Chemical Engineering, University of Maribor, Smetanova ul. 17, 2000 Maribor, SloveniaNational Laboratory for Health, Environment and Food, Prvomajska ul. 1, 2000 Maribor, SloveniaCenter for Human Molecular Genetics and Pharmacogenomics, Faculty of Medicine, University of Maribor, Taborska ul. 8, 2000 Maribor, SloveniaCenter for Human Molecular Genetics and Pharmacogenomics, Faculty of Medicine, University of Maribor, Taborska ul. 8, 2000 Maribor, SloveniaLaboratory for Separation Processes and Product Design, Faculty of Chemistry and Chemical Engineering, University of Maribor, Smetanova ul. 17, 2000 Maribor, SloveniaLaboratory for Analytical Chemistry and Industrial Analysis, Faculty of Chemistry and Chemical Engineering, University of Maribor, Smetanova ul. 17, 2000 Maribor, SloveniaLaboratory for Separation Processes and Product Design, Faculty of Chemistry and Chemical Engineering, University of Maribor, Smetanova ul. 17, 2000 Maribor, SloveniaDepartment of Cardiology and Angiology, University Clinical Center Maribor, Ljubljanska ul. 5, 2000 Maribor, SloveniaLaboratory for Separation Processes and Product Design, Faculty of Chemistry and Chemical Engineering, University of Maribor, Smetanova ul. 17, 2000 Maribor, SloveniaThe prevention and treatment of skin diseases remains a major challenge in medicine. The search for natural active ingredients that can be used to prevent the development of the disease and complement treatment is on the rise. Natural extracts of ginger and hemp offer a wide range of bioactive compounds with potential health benefits. This study evaluates the effectiveness of hemp and ginger extract as a supportive treatment for skin diseases. It reports a synergistic effect of hemp and ginger extract. The contents of cannabinoids and components of ginger are determined, with the highest being CBD (587.17 ± 8.32 µg/g) and 6-gingerol (60.07 ± 0.40 µg/g). The minimum inhibitory concentration for <i>Staphylococcus aureus</i> (156.5 µg/mL), <i>Escherichia coli</i> (625.2 µg/mL) and <i>Candida albicans</i> (78.3 µg/mL) was also analyzed. Analysis of WM-266-4 cells revealed the greatest decrease in metabolic activity in cells exposed to the extract at a concentration of 1.00 µg/mL. Regarding the expression of genes associated with cellular processes, melanoma aggressiveness, resistance and cell survival, a significant difference was found in the expression of <i>ABCB5</i>, <i>CAV1</i> and <i>S100A9</i> compared with the control (cells not exposed to the extract).https://www.mdpi.com/1420-3049/27/22/7694hempgingerextractskin diseasesWM-266-4 |
| spellingShingle | Taja Žitek Dragana Bjelić Petra Kotnik Andrej Golle Staša Jurgec Uroš Potočnik Željko Knez Matjaž Finšgar Ivan Krajnc Igor Krajnc Maša Knez Marevci Natural Hemp-Ginger Extract and Its Biological and Therapeutic Efficacy Molecules hemp ginger extract skin diseases WM-266-4 |
| title | Natural Hemp-Ginger Extract and Its Biological and Therapeutic Efficacy |
| title_full | Natural Hemp-Ginger Extract and Its Biological and Therapeutic Efficacy |
| title_fullStr | Natural Hemp-Ginger Extract and Its Biological and Therapeutic Efficacy |
| title_full_unstemmed | Natural Hemp-Ginger Extract and Its Biological and Therapeutic Efficacy |
| title_short | Natural Hemp-Ginger Extract and Its Biological and Therapeutic Efficacy |
| title_sort | natural hemp ginger extract and its biological and therapeutic efficacy |
| topic | hemp ginger extract skin diseases WM-266-4 |
| url | https://www.mdpi.com/1420-3049/27/22/7694 |
| work_keys_str_mv | AT tajazitek naturalhempgingerextractanditsbiologicalandtherapeuticefficacy AT draganabjelic naturalhempgingerextractanditsbiologicalandtherapeuticefficacy AT petrakotnik naturalhempgingerextractanditsbiologicalandtherapeuticefficacy AT andrejgolle naturalhempgingerextractanditsbiologicalandtherapeuticefficacy AT stasajurgec naturalhempgingerextractanditsbiologicalandtherapeuticefficacy AT urospotocnik naturalhempgingerextractanditsbiologicalandtherapeuticefficacy AT zeljkoknez naturalhempgingerextractanditsbiologicalandtherapeuticefficacy AT matjazfinsgar naturalhempgingerextractanditsbiologicalandtherapeuticefficacy AT ivankrajnc naturalhempgingerextractanditsbiologicalandtherapeuticefficacy AT igorkrajnc naturalhempgingerextractanditsbiologicalandtherapeuticefficacy AT masaknezmarevci naturalhempgingerextractanditsbiologicalandtherapeuticefficacy |